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Chemical composition, antioxidant properties, and sensory aspects of gingerbread enriched with green coffee and cascara

2025, Amotoe-Bondzie, Anthony, Osei, Emmanuel Duah, Ivanišová, Eva, Dávidová, Kristína, Harangozo, Ľuboš, Čech, Matej, Árvay, Július, Kunecová, Daniela, Kucharski, Jan Jakub, Kowalczewski, Przemysław Łukasz

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Great potential of flaxseed mucilage

2024, Kučka, Matúš, Harenčár, Ľubomír, Ražná, Katarína, Nôžková, Janka, Kowalczewski, Przemysław Łukasz, Deyholos, Michael, Dziedzic, Krzysztof, Rybicka, Iga, Zembrzuska, Joanna, Kačániová, Miroslava, Ivanišová, Eva, Gažo, Ján, Čerteková, Simona, Tomka, Marián

AbstractMucilage is one of the key components of flaxseed (which is also called linseed), and exhibits unique functional properties such as high water-holding capacity, and foamability. As a result, flaxseed mucilage has applications in food, agriculture, medicine and other industries. The composition and functional properties of mucilage vary between genotypes. To explore this variation, we characterized the mucilage of nine diverse varieties of flaxseed according a range of parameters including: morphology, mucilage content, fibre content, minerals, phenolic acids, polyphenols, antimicrobial activity, and antioxidant activity. We found significant differences between the genotypes in almost all of the parameters tested. Furthermore, we found a strong correlation between several of these parameters, including antioxidant activity, hydroxycinnamic acid, and sugar content. Antimicrobial activity against Gram positive bacteria was correlated with fibre content and potassium content, while chlorogenic acid content was correlated with suppression of Gram negative bacteria. These results provide a framework for crop improvement further development of functional and value-added products from flaxseed.

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Phytochemical analysis of Bienertia sinuspersici extract and its antioxidant and antimicrobial activities

2024, Aamer, Helmy A., Al-Askar, Abdulaziz A., El-Tanbouly, Rania, Abdelwahab, Eman A., Kowalczewski, Przemysław Łukasz, El-Messeiry, Sarah, Abdelkhalek, Ahmed, Behiry, Said

Abstract Natural products derived from plants are emerging as a valuable resource for a range of antimicrobial agents in sustainable agriculture. The current work looks for the first time at the antimicrobial properties of a methanolic extract of Bienertia sinuspersici against phytopathogenic microorganisms in vitro, using disk diffusion and food poisoning techniques. The phytochemical analysis revealed the presence of total phenols, flavonoids, and antioxidant activity measured at 34.2, 20.6, and 20.1 mg/g, respectively. Furthermore, the concentrations of tannins, saponins, and alkaloids were documented at 17.6, 9.8, and 3.2 mg/g, respectively. The high-performance liquid chromatography examination of polyphenolic chemicals revealed that gallic acid was the most abundant compound at 2.22 mg/g, whereas methyl gallate was the least abundant at 0.021 mg/g. Gas chromatography–mass spectrometry analysis revealed that the primary components were oleic acid, n-hexadecanoic acid, and methyl ester of 11-octadecenoic acid. The extract had a maximal inhibitory percentage of 57.8% against Fusarium oxysporum at 300 µg/mL, 60.4% against Rhizoctonia solani at 500 µg/mL, and 88.2% against Botrytis cinerea. On the other hand, the extract demonstrated inhibition of Pectobacterium atrosepticum growth by 10.67 mm at a concentration of 100 µg/mL, while Ralstonia solanacearum was inhibited by 9.76 mm and Pectobacterium carotovorum by 9 mm. Overall, the extract of B. sinuspersici demonstrated promising efficacy as antibacterial and antifungal agents against different plant pathogens. Therefore, it could offer a sustainable and environmentally friendly alternative for managing plant diseases.

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Antioxidant, Antimicrobial, and Anti-Insect Properties of Boswellia carterii Essential Oil for Food Preservation Improvement

2023, Borotová, Petra, Čmiková, Natália, Galovičová, Lucia, Vukovic, Nenad L., Vukic, Milena D., Tvrdá, Eva, Kowalczewski, Przemysław, Kluz, Maciej Ireneusz, Puchalski, Czeslaw, Schwarzová, Marianna, Bakay, Ladislav, Kačániová, Miroslava

Boswellia carterii, known as frankincense, is a fragrant medicinal plant. The essential oil from this plant is often used in traditional medicine or aromatherapy. Due to its positive properties, it has potential applications as an antimicrobial agent in medicine and the food industry. The aim of this study was to evaluate the antimicrobial, anti-insect, and antioxidant activities of frankincense essential oil (FEO). The composition of volatile compounds was determined by GC/MS, and the main components were found to be α-pinene (37.0%), α-limonene (19.8%), and p-cymene (6.3%). The antioxidant activity was evaluated with DPPH and ABTS methods with the resulting inhibition of 73.88 ± 0.35% of DPPH radical (6.27 ± 0.17 TEAC) and 97.09 ± 0.48% of ABTS radical cation (5.21 ± 0.07 TEAC). The antimicrobial activity was the strongest against Candida species with inhibition zones in the range of 38.00–44.00 mm and MIC 50 and MIC 90 values of 11.72 and 12.58 μL/mL, respectively. The inhibition activity of the vapor phase was the highest against G+ and G− bacteria growing on a carrot with inhibition ranging from 65.14 to 93.67%. The anti-insect activity against O. lavaterae was determined as 100% at 100% concentration and 50% at 25% concentration. The degradation of biofilm was tested with MALDI-TOF MS, and changes in the protein profile were observed.

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Antioxidant and antimicrobial activities of Salsola imbricata methanolic extract and its phytochemical characterization

2024, Aamer, Helmy A., Elalem, Saad F., Al-Askar, Abdulaziz A., Sharaf, Omaima A., Gaber, Mahmoud A., Kowalczewski, Przemysław Łukasz, Behiry, Said, Abdelkhalek, Ahmed

Abstract Methanolic extract from Salsola imbricata was investigated for its phytochemical content, antioxidant, and antimicrobial properties against phytopathogenic fungi and bacteria. Phytochemical analysis revealed the presence of saponin, tannins, and alkaloids with 1.25%, 18.8 mg catechin/g of extract, and 9.12%, respectively. Total flavonoid content was 20.8 mg quercetin equivalent/g while total phenolic content was 202 mg gallic acid equivalent/g. Antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl assay resulted in an IC50 value of 48.61 µg/mL, while the phosphomolybdenum method yielded a value of 215.43 mg ascorbic acid equivalent/g of extract. The highest phenolic acids detected in the extract were gallic acid (712.97 µg/g), syringic acid (742.7 µg/g), and caffeic acid (474.70 µg/g) according to high-performance liquid chromatography analysis. Palmitic acid (28.38%) dominated the fatty acids identified by gas chromatography–mass spectrometry, while stigmasterol (8.34%) was the most abundant steroid. At a concentration of 3 mg/mL, the extract showed strong antibacterial activity against Pectobacterium carotovorum (10.50 mm), Ralstonia solanacearum (9.93 mm), and Pectobacterium atrosepticum (8.37 mm). Additionally, the extract significantly suppressed fungal growth of Rhizoctonia solani (38.22%) and Fusarium oxysporum (33.56%) but showed lower activity toward Botrytis cinerea (13.33%) at 5 mg/mL. In conclusion, S. imbricata extract exhibited promising antioxidant and antimicrobial properties, making it a potential candidate for further exploration in agricultural applications.