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Effect of refining and fractionation processes on minor components, fatty acids, antioxidant and antimicrobial activities of shea butter

2023, Abdel-Razek, Adel G., Abo-Elwafa, Ghada A., Al-Amrousi, Eman F., Badr, Ahmed N., Hassanein, Minar Mahmoud M., Qian, Ying, Siger, Aleksander, Grygier, Anna, Radziejewska-Kubzdela, Elżbieta, Rudzińska, Magdalena

Shea butter is becoming increasingly popular in foods, cosmetics and pharmaceutical products. This work aims to study the effect of the refining process on the quality and stability of fractionated and mixed shea butters. Crude shea butter, refined shea stearin, olein and their mixture (1:1 w/w) were analyzed for fatty acids, triacylglycerol composition, peroxide value (PV), free fatty acids (FFA), phenolic (TPC), flavonoid (TFC), unsaponifiable matter (USM), tocopherol and phytosterol content. Additionally, the oxidative stability, radical scavenging activity (RSA), antibacterial and antifungal activities were evaluated. The two main fatty acids in the shea butter samples were stearic and oleic. The refined shea stearin showed lower PV, FFA, USM, TPC, TFC, RSA, tocopherol and sterol content than crude shea butter. A higher EC50 was observed, but antibacterial activity was much lower. The refined olein fraction was characterized by lower PV, FFA and TFC in comparison with crude shea butter, but USM, TPC, RSA, EC50, tocopherol and sterol content was unchanged. The antibacterial activity was higher, but the antifungal activity was lower than those of crude shea butter. When both fractions were mixed, their fatty acid and triacylglycerol composition were similar to those of crude shea butter, but other parameters were different.

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Stigmasterol and its esters encapsulated in liposomes: Characterization, stability, and derivative formation

2025, Rudzińska, Magdalena, Cieślik-Boczula, Katarzyna, Grygier, Anna, Kmiecik, Dominik, Dwiecki, Krzysztof, Jarzębski, Maciej

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Analysis of phytosterols encapsulated in pegylated liposomes

2025, Igielska-Kalwat, Joanna, Rudzińska, Magdalena, Grygier, Anna, Kmiecik, Dominik, Cieślik-Boczula, Katarzyna, Dwiecki, Krzysztof, Smułek, Wojciech

Abstract Dipalmitoylphosphatidylcholine (DPPC) lipids were encapsulated in PEGylated liposomes with free stigmasterol (ST), stigmasterol myristate (ME), and stigmasterol oleate (OE). Their quality was assessed using TEM, zeta potential, and hydrodynamic diameter measurements. The liposomes were heated to 60 Â°C and 180 Â°C. The degradation of stigmasterol and fatty acids was considered, as was derivative formation. The results show that the liposomes fulfilled their intended function. The ST liposomes were smallest, while the ME liposomes were similar in size to the OE liposomes. The degree of degradation of the compounds encapsulated in the liposomes depended on their structure. After heating the samples to 60 Â°C, the extent of stigmasterol degradation ranged from 3.5% in ST to 4.3% in ME and 6.5% in OE. After heating to 180 Â°C, the lowest level of stigmasterol degradation was observed for OE (7.3%), while degradation in ST and ME reached 13.4% and 10.1%, respectively. The high level of oxyphytosterols in all samples heated to 180 Â°C raised concerns. The oxyphytosterol (SOP) content of the liposomes heated to 60 Â°C ranged from 23.2 mg/g in those with free stigmasterol to 6.3 mg/g and 6.4 mg/g in the liposomes with stigmasterol myristate and stigmasterol oleate, respectively. After heating to 180 Â°C, the total SOP content was significantly higher, ranging from 88.7 mg/g for OE to 7.4 and 29.6 mg/g for ME and ST, respectively.

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Comprehensive characteristic of lipid fraction as a distinguishing factor of three lupin seed species

2023, Siger, Aleksander, Grygier, Anna, Czubiński, Jarosław

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Lipophilic profile of mature seeds of unconventional edible tree legumes

2023, Grygier, Anna, Chakradhari, Suryakant, Ratusz, Katarzyna, Rudzińska, Magdalena, Patel, Khageshwar Singh, Lazdiņa, Danija, Segliņa, Dalija, Górnaś, Paweł

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Changes in physicochemical, textural, and sensorial properties of pork meatballs made with the addition of hemp oil during storage

2025, Montowska, Magdalena, Kotecka-Majchrzak. Klaudia, Kasałka-Czarna, Natalia, Mikołajczak, Beata, Spychaj, Anita, Grygier, Anna

This research aimed to evaluate the quality characteristics of cooked and vacuum-packed meatballs reformulated with cold-pressed hempseed oil as a partial pork substitute (0.8%, 2.5%, 4.2%, and 7.5%) during 12 days of storage. The water activity, cooking, and storage losses increased with a higher content of hemp oil ( P < 0.05). The total saturated fatty acids were reduced by 37.6%, whereas the polyunsaturated fatty acids content improved by 96.1%. Hemp oil addition decreased protein and lipid oxidation during the storage period ( P < 0.05). The inhibition effect on carbonyl content reached 34.9% and on TBARS values reached 17.5%. Sensory analysis revealed no significant changes to the texture, odour, and taste attributes over 12 days of storage in vacuum packaging. The results indicate that cold-pressed hemp oil can be an alternative ingredient for the production of meat products with improved nutritional value, particularly by enriching them with n-3 α-linolenic fatty acid.

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Assessment of Chemical Composition and Oxidative Stability in Cold-pressed Date Kernel Oil from Phoenix dactylifera L.: Implications for Food Quality and Sustainability

2025, Ksibi, Nour, Yeddes, Walid, Symoniuk, Edyta, Hammami, Majdi, Siger, Aleksander, Grygier, Anna, Tomaszewska-Gras, Jolanta, Janowicz, Monika, Wannes, Wissem Aidi, Wroniak, Malgorzata, Ratusz, Katarzyna, Rebey, Ines Bettaieb, Tounsi, Moufida Saidani

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Comparative analysis of lipophilic phytochemicals in seed oils of six aromatic plant species

2025, Grygier, Anna, Chakradhari, Suryakant, Rudzińska, Magdalena, Wroniak, Małgorzata, Segliņa, Dalija, Patel, Khageshwar Singh, Soliven, Arianne, Górnaś, Paweł