2025, Skrypnik, Katarzyna, Selvan, Damla, Suliburska, Joanna

2022, Wesolek, A., Skoracka, K., Skrypnik, Katarzyna, Suliburska, Joanna, Bogdanski, P., Szulinska, M., Skrypnik, D.

2025, Kręgielska-Narożna, Matylda, Miller-Kasprzak, Ewa, Matuszewska, Eliza, Matysiak, Jan, Skrypnik, Katarzyna, Łoniewski, Igor, Szulińska, Monika, Bogdański, Paweł

The composition and functions of the gut microbiota can affect the development of obesity and metabolic disorders. Proteomics analysis has become important tool for characterizing the proteome and identifying serum biomarkers associated with specific conditions in different groups of patients. The aim of this study was to gain insight into the pathophysiological aspects of obesity and the relation between comorbidities and the gut microbiota by characterizing the protein profile in obese post-menopausal women before and after multi-species probiotic supplementation. MALDI-TOF MS analyses were performed in serum samples from the placebo group 24, from the low-dose probiotic group 24, and 23 from the high-dose probiotic group. Paired comparison analysis of protein–peptide patterns derived from patients at two time points (i.e., before and after treatment) revealed several peaks changing under treatment. Complement C3, Fibrinogen alpha chain, Coagulation factor XII, Tubulin beta-3 chain, Fibrinogen beta chain, Inter-alpha-trypsin inhibitor heavy chain H4, and Apolipoprotein A-I were identified. Depending on the administered dose, slightly different alterations were observed. In summary, the obtained results do not allow us to draw a conclusion regarding the influence of probiotic supplementation on peptide profile in obese post-menopausal women. However some of identified proteins could take part in the complex mechanism involving obesity, dysbiosis, and inflammation. Registration number: NCT03100162

2023, Skrypnik, Damian, Skrypnik, Katarzyna, Suliburska, Joanna, Bogdański, Paweł

SummaryBy 2030, it is expected that a billion people will have suffer from obesity. Adipose tissue synthesizes leptin, an adipokine that affects cardiovascular risk. Leptin intensifies the synthesis of vascular endothelial growth factor (VEGF). Our study reviews recent reports on leptin–VEGF crosstalk in obesity and related disorders. PubMed, Web of Science, Scopus, and Google Scholar were searched. One hundred and one articles involving human, animal, and in vitro research were included. In vitro studies show the crucial role of interaction between endothelial cells and adipocytes and hypoxia as a factor that intensifies leptin's effects on VEGF. Leptin–VEGF crosstalk promotes the progression of cancer. The animal research reveal that a high‐fat diet enhances leptin and VEGF crosstalk. Genetic and epigenetic mechanisms and procreator‐offspring programming may be involved in leptin–VEGF crosstalk. Some female‐specific characteristics of leptin–VEGF relation in obesity were observed. The human studies have shown that increased leptin and VEGF synthesis and leptin–VEGF crosstalk are factors linking obesity with elevated cardiovascular risk. The studies of the last 10 years documented a range of significant aspects of leptin–VEGF crosstalk specific for obesity and related disorders, shedding new light on the link between obesity and increased cardiovascular risk.