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SposĂłb przygotowania inokulum bakterii celulolitycznych do wytwarzania biopreparatĂłw poprawiajÄ cych wĆaĆciwoĆci gleby
Product Development Study of Freeze-Dried Apples Enriched with Sea Buckthorn Juice and Calcium Lactate
Enriched or fortified foods are typically linked to ultra-processed foods, limiting the choice of functional food in the market. Addressing the market potential, particularly the elder population with osteoporosis, the functional food industry should consider developing a healthy snack enriched with bioactive substances. This study aimed to produce freeze-dried Polish Gala apple with improved antioxidant properties and calcium content via impregnation or osmotic dehydration process. The solutions containing various concentrations of sea buckthorn (SB) juice and inulin were prepared at different temperatures and times, then analyzed by response surface regression modelling. Subsequently, the effect of the addition of 0â6% calcium lactate (CaL) on antioxidant properties and calcium content was also studied. Freeze-dried apple, after impregnation with 93.8% SB juice, 0:100 inulinâSB juice ratio, at 30 °C for 120 min, with the addition of 4% CaL (hereafter called â4% CaLâ treatment), possessed a minimum yet acceptable loss of antioxidant properties and increased calcium content (2209.13 mg Ca/100 g). UPLC-PDA revealed the altered compositions of phenolics (flavonols were dominated by isorhamnetin-3-O-glucoside and isorhamnetin-3-O-rutinoside) and carotenoids in 4% CaL. The 4% CaL also exhibited lower polyphenol oxidase and peroxidase activities, moderate sensory acceptability with soft texture, and better nutritional values with lower calories when compared to the controls. This work is a scalable study, covering aspects of process design, physicochemical, nutritional, and enzymatic properties, as well as sensory profiling, which has potential for industrial implementation.
Optimization and Modeling of Citrobacter freundii AD119 Growth and 1,3-Propanediol Production Using Two-Step Statistical Experimental Design and Artificial Neural Networks
1,3-propanediol (1,3-PD) has a wide range of industrial applications. The most studied natural producers capable of fermenting glycerol to 1,3-PD belong to the genera Klebsiella, Citrobacter, and Clostridium. In this study, the optimization of medium composition for the biosynthesis of 1,3-PD by Citrobacter freundii AD119 was performed using the one-factor-at-a-time method (OFAT) and a two-step statistical experimental design. Eleven mineral components were tested for their impact on the process using the PlackettâBurman design. MgSO4 and CoCl2 were found to have the most pronounced effect. Consequently, a central composite design was used to optimize the concentration of these mineral components. Besides minerals, carbon and nitrogen sources were also optimized. Partial glycerol substitution with other carbon sources was found not to improve the bioconversion process. Moreover, although yeast extract was found to be the best nitrogen source, it was possible to replace it in part with (NH4)2SO4 without a negative impact on 1,3-PD production. As a part of the optimization procedure, an artificial neural network model of the growth of C. freundii and 1,3-PD production was developed as a predictive tool supporting the design and control of the bioprocess under study.
Biocontrol of Cercospora leaf spot in sugar beet by a novel Bacillus velezensis KT27 strain: Enhanced antifungal activity and growth promotion in laboratory and field conditions
Diseases in crops are a major contributor to yield reduction and economic losses. Cercospora leaf spot (CLS), caused by Cercospora beticola, is among the most severe diseases affecting sugar beet and other crops. The increasing resistance of C. beticola to conventional chemical fungicides, along with their excessive application, exacerbates environmental pollution. This study investigates the antagonistic activity of a newly isolated strain, Bacillus velezensis KT27, against Cercospora beticola, Rhizoctonia cerealis, and Fusarium oxysporum under laboratory conditions. The bacteriumâs ability to produce lipopeptides (surfactin, iturin, and fengycin) and solubilize phosphorus, potassium, and zinc was also assessed. In vitro assays revealed that B. velezensis KT27 effectively inhibited C. beticola growth (60.2%), though it exhibited lower antagonistic activity against R. cerealis (22.5%) and F. oxysporum (15.5%). The elimination of bacterial biomass by centrifugation and the use of sterile supernatant reduced antifungal activity by more than 3.5-fold for all tested fungi, highlighting the importance of direct bacterial interactions. Notably, the antagonistic effect of B. velezensis KT27 against C. beticola significantly increased when bacterial cultures were supplemented with thermally inactivated fungal biomass of C. beticola especially R. cerealis. Field experiments demonstrated the high efficacy of B. velezensis KT27 biological control agent, particularly when induced by R. cerealis. The level of CLS protection achieved with the bacterial treatment was only 9.1% lower than that obtained using a combination of three chemical fungicides. Additionally, the biocontrol agent positively influenced sugar beet growth, leading to a root yield increase of up to 15.2% compared to the untreated control. These findings highlight the potential of B. velezensis KT27 as an effective and environmentally sustainable biocontrol agent against CLS in sugar beet cultivation.
The study of relationship between taste and wheat bran protein isolate fortification during high-protein yogurt manufacturing
SposĂłb i urzÄ dzenie do regeneracji wody, zwĆaszcza stosowanej do wymywania polimerowych pĆyt fleksograficznych
âMother(Nature) knows bestâ â hijacking nature-designed transcriptional programs for enhancing stress resistance and protein production in Yarrowia lipolytica; presentation of YaliFunTome database
Abstract Background In the era of rationally designed synthetic biology, heterologous metabolites production, and other counter-nature engineering of cellular metabolism, we took a step back and recalled that âMother(-Nature) knows bestâ. While still aiming at synthetic, non-natural outcomes of generating an âover-production phenotypeâ we dug into the pre-designed transcriptional programs evolved in our host organismâYarrowia lipolytica, hoping that some of these fine-tuned orchestrated programs could be hijacked and used. Having an interest in the practical outcomes of the research, we targeted industrially-relevant functionalitiesâstress resistance and enhanced synthesis of proteins, and gauged them over extensive experimental designâs completion. Results Technically, the problem was addressed by screening a broad library of over 120 Y. lipolytica strains under 72 combinations of variables through a carefully pre-optimized high-throughput cultivation protocol, which enabled actual phenotype development. The abundance of the transcription program elicitorsâtranscription factors (TFs), was secured by their overexpression, while challenging the strains with the multitude of conditions was inflicted to impact their activation stratus. The data were subjected to mathematical modeling to increase their informativeness. The amount of the gathered data prompted us to present them in the form of a searchable catalog â the YaliFunTome database (https://sparrow.up.poznan.pl/tsdatabase/)âto facilitate the withdrawal of biological sense from numerical data. We succeeded in the identification of TFs that act as omni-boosters of protein synthesis, enhance resistance to limited oxygen availability, and improve protein synthesis capacity under inorganic nitrogen provision. Conclusions All potential users are invited to browse YaliFunTome in the search for homologous TFs and the TF-driven phenotypes of interest.