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Improving the Selection Efficiency of Breeding Material within Interspecific Brassicaceae Hybrids with Genomic Prediction and Phenotyping
Various molecular markers can be applied to accelerate the breeding process of Brassicaceae plants. The aim of the present study was to assess the usefulness of available markers connected to blackleg resistance and to screen for markers linked to genes influencing major morphological characteristics, suitable for the further selection of Brassicaceae hybrids. Combining the field and molecular results allowed for the selection of useful SSR markers, including mstg004 and mstg027 markers connected to the color of the flowers and marker mstg038 associated with stem color. The field trails were also conducted to investigate the level of blackleg resistance, which permitted the selection of rapeseed hybrids with B. fruticulosa, B. carinata, and S. alba exhibiting the lowest infestation. Furthermore, the functionality of some of the resistance-linked markers was confirmed. The importance of interspecific hybridization and the use of marker-assisted selection are discussed, and the high utility of presented markers in further studies is highlighted.
Marker assisted selection in Brassica napus breeding for stem canker (Leptosphaeria ssp.) resistance.
Genetic Relationship of Brassicaceae Hybrids with Various Resistance to Blackleg Is Disclosed by the Use of Molecular Markers
Brassica napus is an important oil source. Its narrow gene pool can be widened by interspecific hybridization with the Brassicaceae species. One of the agronomically important traits, that can be transferred through the hybridization, is the resistance to blackleg, a dangerous disease mainly caused by Leptosphaeria maculans. Hybrid individuals can be analyzed with various molecular markers, including Simple Sequence Repeats (SSR). We investigated the genetic similarity of 32 Brassicaceae hybrids and 19 parental components using SSR markers to reveal their genetic relationship. Furthermore, we compared the field resistance to blackleg of the interspecific progenies. The tested set of 15 SSR markers proved to be useful in revealing the genetic distances in the Brassicaceae hybrids and species. However, genetic similarity of the studied hybrids could not be correlated with the level of field resistance to L. maculans. Moreover, our studies confirmed the usefulness of the Brassicaceae hybrids in terms of blackleg management.
Novel Brassica hybrids with different resistance to Leptosphaeria maculans reveal unbalanced rDNA signal patterns
AbstractHybridization ofBrassica napuswith various Brassicaceae species can result in obtaining new forms with increased resistance to blackleg, a dangerous disease caused mainly byLeptosphaeria maculans. In this study, we aimed to correlate the field resistance of selectedBrassicahybrids to blackleg with chromosomal structure revealed by Fluorescencein situhybridization. Tested genotypes varied in the number of chromosomes and rDNA signals. The greatest variation was observed for A1-type chromosomes. Field evaluation also revealed significant differences inL. maculansresistance. Performed analyses allowed to distinguish threeB. napusĂBrassica fruticulosagenotypes in which variable patterns of chromosomal structure might be connected to field resistance. However, a more thorough study, including the detection of all A-genome chromosomes, is required.