Improving the Selection Efficiency of Breeding Material within Interspecific Brassicaceae Hybrids with Genomic Prediction and Phenotyping

Various molecular markers can be applied to accelerate the breeding process of Brassicaceae plants. The aim of the present study was to assess the usefulness of available markers connected to blackleg resistance and to screen for markers linked to genes influencing major morphological characteristics, suitable for the further selection of Brassicaceae hybrids. Combining the field and molecular results allowed for the selection of useful SSR markers, including mstg004 and mstg027 markers connected to the color of the flowers and marker mstg038 associated with stem color. The field trails were also conducted to investigate the level of blackleg resistance, which permitted the selection of rapeseed hybrids with B. fruticulosa, B. carinata, and S. alba exhibiting the lowest infestation. Furthermore, the functionality of some of the resistance-linked markers was confirmed. The importance of interspecific hybridization and the use of marker-assisted selection are discussed, and the high utility of presented markers in further studies is highlighted.