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Methoprene, a Juvenile Hormone Analog, Causes Winter Diapause Elimination in Univoltine Bee Species Osmia bicornis L.
Osmia bicornis syn. O. rufa is a univoltine bee species in which adults fly in spring and the offspring overwinter as cocooned imagoes. The flight period of solitary bees is short, so methods of control for development and emergence time are needed to synchronize the activity of managed pollinators with blooming. In our study, we tested the effectiveness of a juvenile hormone analog for the prevention of winter diapause. Bees developed in settled nests outdoors or in the laboratory (22 °C) until the end of the pre-pupa stage, then cocoons were removed from the nest cells and treated with a JH analog—methoprene—during the pupa and young imago stages. Then, bees were activated at 25 °C until the adults left the cocoons. Topical application of methoprene to the cocoon at the pupa or imago stage induced the emergence of some adult bees in the pre-diapause period, while no adults emerged when the bees were not treated with methoprene. Most adults emerged (about 50%) when treated with methoprene on 3-week-old cocooned imagoes. Bees treated in the pupal stage had a lower emergence rate (20–30%), but adult bees emerged earlier. The emergence time of adults for the laboratory group was, on average, from 70 to 91 days, and that for outdoor groups was from 57 to 72 days.
Use of Osmia bicornis L. for pollination of Cyclamen persicum Mill. cultivated in greenhouse environment during winter period
Abstract In this study, the pollinating potential of the red mason bee (Osmia bicornis syn. O. rufa) in seed production of a major ornamental plant - Persian cyclamen (Cyclamen persicum), was evaluated under horticultural farm conditions. The study was conducted under a greenhouse environment for five months, from October to the end of February. The juvenile hormone analogue methoprene supported the termination of bee diapause. Experimental results from Osmia pollination plots were compared with control plots that applied the hand-pollination method. Plants with flowers pollinated by the solitary bee had significantly more fruits than those pollinated by the hand method in November, December and February. In addition, significantly more seeds per capsule were produced by plants pollinated by O. bicornis from December to February. Finally, plants pollinated by Osmia bees produced more seeds than those hand-pollinated at all experimental months. These results showed that if the diapause of O. bicornis is successfully broken, this bee can be a very efficient pollinator in greenhouse environments, even during winter.
First detection of Crenosoma vulpis in Red Foxes Vulpes vulpes in Western Poland
Summary Crenosoma vulpis is a nematode from the superfamily Metastrongylidea, which parasitizes the respiratory tracts of carnivores, primarily red foxes ( Vulpes vulpes ), and occasionally domestic dogs ( Canis familiaris ). This study aimed to assess the presence of C. vulpis in red foxes in western Poland, an area where its occurrence had not been previously documented. We examined lung tissue from 47 red foxes obtained during the regular hunting season (2017 – 2018) using the Baermann method to detect the presence of nematode larvae. Larvae, identified based on morphology as C. vulpis , were found in 12.8 % of the foxes (6/47; 95 % CL = 4.8 – 25.7 %). This is the first confirmed report of Crenosoma vulpis in red foxes in Poland, highlighting the potential need for further epidemiological studies on its distribution and impact on both wildlife and domestic animals.