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Development of Delivery Systems with Prebiotic and Neuroprotective Potential of Industrial-Grade Cannabis sativa L.
This study delves into the transformative effects of supercritical carbon dioxide (scCO2) cannabis extracts and prebiotic substances (dextran, inulin, trehalose) on gut bacteria, coupled with a focus on neuroprotection. Extracts derived from the Białobrzeska variety of Cannabis sativa, utilising supercritical fluid extraction (SFE), resulted in notable cannabinoid concentrations (cannabidiol (CBD): 6.675 ± 0.166; tetrahydrocannabinol (THC): 0.180 ± 0.006; cannabigerol (CBG): 0.434 ± 0.014; cannabichromene (CBC): 0.490 ± 0.017; cannabinol (CBN): 1.696 ± 0.047 mg/gD). The assessment encompassed antioxidant activity via four in vitro assays and neuroprotective effects against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). The extract boasting the highest cannabinoid content exhibited remarkable antioxidant potential and significant inhibitory activity against both enzymes. Further investigation into prebiotic deliveries revealed their proficiency in fostering the growth of beneficial gut bacteria while maintaining antioxidant and neuroprotective functionalities. This study sheds light on the active compounds present in the Białobrzeska variety, showcasing their therapeutic potential within prebiotic systems. Notably, the antioxidant, neuroprotective, and prebiotic properties observed underscore the promising therapeutic applications of these extracts. The results offer valuable insights for potential interventions in antioxidant, neuroprotective, and prebiotic domains. In addition, subsequent analyses of cannabinoid concentrations post-cultivation revealed nuanced changes, emphasising the need for further exploration into the dynamic interactions between cannabinoids and the gut microbiota.
Preliminary Studies on the Effect of Soil Conditioner (AMP) Application on the Chemical and Microbiological Properties of Soil under Winter Oilseed Rape Cultivation
This study analyzed the effect of the application of a soil conditioner under the trade name of the Agro Mineral Product (AMP) in the winter rapeseed cultivation on the bacterial and fungal abundance, ion concentrations, and electrolytic conductivity of the soil solution. It was demonstrated that the AMP influenced changes in the total abundance of the culturable fractions of the soil bacteria and fungi at each of the tested time points. A stimulatory effect of the preparation on the growth of the soil bacteria and an inhibitory effect on the development of the fungi was observed, particularly at doses of 4 and 8 t·ha−1. A dose of 12 t·ha−1 proved to be the least effective in relation to the development of the soil microbiome. Increasing the AMP fertilization dose above 4 t·ha−1 caused changes in the chemistry of the soil solution (pH, EC, HCO3−, K+, and PO4-P). It is worth noting that this primarily resulted in decreases in the amounts of mobile forms of potassium (from 40.4 mg·dm−3 in the control to 26.7 mg·dm−3 at the 8 t·ha−1 dose) and orthophosphate as phosphorus (from −6.00 mg·dm−3 in the control to 3.75 mg·dm−3 at the 8 t·ha−1 dose) in the soil solution, which resulted in a reduction in the yield of the winter rapeseed (from 4.76 t·ha−1 in the control to 4.61 t·ha−1 at the 8 t·ha−1 and 4.43 t·ha−1 at the 12 t·ha−1 AMP dose).