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Ocena jakościowych i ilościowych zmian mikrobiomu bakteryjnego w procesie beztlenowego rozkładu materii organicznej
Sustainable Methods of Soybean Cultivation in Poland
Many countries in Europe are struggling with a shortage of feed protein; moreover, efforts are being made to limit the import of post-extraction soybean meal, most often from GMO crops. To achieve the above assumptions, varietal progress is necessary and, above all, breeding work should aim at greater adaptation to regional conditions. This study was designed to evaluate the potential for growing Ukrainian soybean ‘Annushka’ in the southeastern Baltic Sea area, in accordance with the application of mineral nitrogen fertilizer and the inoculation of seeds with Bradyrhizobium japonicum. Soybean ‘Annushka’ yielded 0.98–1.68 t ha−1 in the conditions of central Poland. Our experiments have shown significant variations in seed, protein, and fat yields over the years. The maximum amounts of these characteristics were recorded in 2017. Nitrogen fertilization combined with seed inoculation with B. japonicum has proven to be an important factor in improving soybean yields; however, it slightly modified the content of organic compounds in seeds. Improvement in seed and protein yields relative to the control amounted, respectively, to Nitragina + 30 kg N ha−1 (58.8%; 72.6%), HiStick® Soy + 30 kg N ha−1 (57.6%; 68.3%), and Nitroflora + 60 kg N ha−1 (57.6%; 71.9%).
Additives Improving the Efficiency of Biogas Production as an Alternative Energy Source - A Review
Additives for anaerobic digestion (AD) can play a significant role in optimizing the process by increasing biogas production, stabilizing the system, and improving digestate quality. The role of additives largely boils down to, among others, enhancing direct interspecies electron transfer (DIET) between microbial communities, resulting in improved syntrophic interactions, adsorption of toxic substances that may inhibit microbial activity, improving microbial activity, and increasing process stability and accelerating the decomposition of complex organic materials, thereby increasing the rate of hydrolysis. Through the aforementioned action, additives can significantly affect AD performance. The function of these materials varies, from enhancing microbial activity to maintaining optimal conditions and protecting the system from inhibitors. The choice of additives should be carefully tailored to the specific needs and conditions of the digester to maximize benefits and ensure sustainability. In light of these considerations, this paper characterizes the most commonly used additives and their combinations based on a comprehensive review of recent scientific publications, including a report on the results of conducted studies. The publication features chapters that describe carbon-based conductive materials, metal oxide nanomaterials, trace metal, and biological additives, including enzymes and microorganisms. It concludes with the chapters summarising reports on various additives and discussing their functional properties, as well as advantages and disadvantages. The presented review is a substantive and concise analysis of the latest knowledge on additives for the AD process. The application of additives in AD is characterized by great potential; hence, the subject matter is very current and future-oriented.
Wpływ nośników w postaci krzemionki i ligniny na różnorodność mikrobiomu bakteryjnego oraz wydajność fermentacji metanowej
The Response of the Mycobiome to the Biofumigation of Replanted Soil in a Fruit Tree Nursery
In a long-term monoculture with fruit trees and tree nurseries, it is necessary to regenerate the soil due to the risk of apple replant disease (ARD). The occurrence of ARD is manifested in the structure of the mycobiome. The assumption of our experiment was that the use of oil radish (Raphanus sativus var. oleifera), white mustard (Sinapis alba), and marigold (Tagetes patula L.) as phytosanitary plants for biofumigation would provide crops with nutrients, improve soil physicochemical properties, and influence the diversity of microbiota, including fungal networks, towards a beneficial mycobiome. Metagenomic analysis of fungal populations based on the hypervariable ITS1 region was used for assessing changes in the soil mycobiome. It showed that biofumigation, mainly with a forecrop of marigold (Tagetes patula L.) (R3), caused an improvement in soil physicochemical properties (bulk density and humus) and the highest increase in the abundance of operational taxonomic units (OTUs) of the Fungi kingdom, which was similar to that of agriculturally undegraded soils, and amounted to 54.37%. In this variant of the experiment, the most OTUs were identified at the phylum level, for Ascomycota (39.82%) and Mortierellomycota beneficial fungi (7.73%). There were no such dependencies in the soils replanted with forecrops of oilseed radish (Raphanus sativus var. oleifera) and white mustard (Sinapis alba). Biofumigation with marigold and oil radish contributed to a reduction in the genus Fusarium, which contains several significant plant-pathogenic species. The percentages of operational taxonomic units (OTUs) of Fusarium spp. decreased from 1.57% to 0.17% and 0.47%, respectively.
Additives Improving the Efficiency of Biogas Production as an Alternative Energy Source—A Review
Additives for anaerobic digestion (AD) can play a significant role in optimising the process by increasing biogas production, stabilising the system and improving digestate quality. The role of additives largely boils down to: (i) enhancing direct interspecies electron transfer (DIET) between microbial communities, resulting in improved syntrophic interactions and methane production rates (e.g. biochar, magnetite and carbon nanotubes), (ii) adsorption of toxic substances that may inhibit microbial activity (e.g. activated carbon, zeolites), (iii) improving microbial activity and increasing process stability (e.g. cobalt, nickel, iron, selenium), (iv) maintaining optimal pH levels for microbial activity (e.g. magnesium oxide), (v) reducing inhibition (the aforementioned adsorbents and conductive substances), (vi) accelerating the decomposition of complex organic materials into simpler compounds that are more easily digested by microorganisms, thereby increasing the rate of hydrolysis (enzymes, including cellulases, proteases and lipases). Through the aforementioned action, additives can significantly affect AD performance. The function of these materials varies, from enhancing microbial activity to maintaining optimal conditions and protecting the system from inhibitors. The choice of additives should be carefully tailored to the specific needs and conditions of the digester to maximise benefits and ensure sustainability. In light of these considerations, this paper characterizes the most commonly used additives and their combinations based on a comprehensive review of recent scientific publications, including a report on the results of conducted studies. The publication features chapters that describe: carbon-based conductive materials, metal oxide nanomaterials, trace metal and biological additives, including enzymes and microorganisms. It concludes with a chapter summarising reports on various additives and discussing their indications for functional systems with determined properties. A notable advantage of this work is the updated literature data, clear summaries, and a substantive description of the performance of the additives discussed.