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Ocena jakościowych i ilościowych zmian mikrobiomu bakteryjnego w procesie beztlenowego rozkładu materii organicznej
Anaerobic Digestion of Food Waste—A Short Review
In recent years, growing environmental awareness, the need to reduce greenhouse gas emissions, and the energy crisis have led many countries to seek alternative energy sources. One of the most promising solutions is biogas production via anaerobic digestion (AD), whose substrate can be organic-rich and easily biodegradable food waste (FW). This waste is a significant part of the global waste problem, and its use for energy production is beneficial to both the environment and the economy. This paper presents important issues concerning the monitoring of the AD process, as well as standard and innovative, for the implementation of this process, technological solutions. The aim of the measures taken to optimise the process is to increase AD efficiency and obtain the highest possible methane content in biogas. Two approaches—pretreatment and anaerobic co-digestion (AcoD)—have been integral to the implementation of AD of food waste for years. They are presented in this paper based on a review of recent research developments. Pretreatment methods are discussed with particular emphasis on mechanical, chemical and biological methods. The AcoD of FW with different organic substrates has been extensively reviewed, as confirmed by numerous studies, where higher buffer capacity and optimum nutrient balance enhance the biogas/methane yields. Attention was also paid to the parameters, operating mode and configurations of anaerobic digesters, with a thorough analysis of the advantages and disadvantages of each solution. The article concludes with a brief presentation of the development perspectives for the discussed FW management method and recommendations.
Biofilm Formation and Genetic Diversity of Microbial Communities in Anaerobic Batch Reactor with Polylactide (PLA) Addition
In this paper, an anaerobic digestion (AD) study was conducted on confectionery waste with granular polylactide (PLA) as a cell carrier. Digested sewage sludge (SS) served as the inoculum and buffering agent of systems. This article shows the results of the analyses of the key experimental properties of PLA, i.e., morphological characteristics of the microstructure, chemical composition and thermal stability of the biopolymer. The evaluation of quantitative and qualitative changes in the genetic diversity of bacterial communities, performed using the state-of-the-art next generation sequencing (NGS) technique, revealed that the material significantly enhanced bacterial proliferation; however, it does not change microbiome biodiversity, as also confirmed via statistical analysis. More intense microbial proliferation (compared to the control sample, without PLA and not digested, CW–control, CW–confectionery waste) may be indicative of the dual role of the biopolymer—support and medium. Actinobacteria (34.87%) were the most abundant cluster in the CW–control, while the most dominant cluster in digested samples was firmicutes: in the sample without the addition of the carrier (CW–dig.) it was 68.27%, and in the sample with the addition of the carrier (CW + PLA) it was only 26.45%, comparable to the control sample (CW–control)—19.45%. Interestingly, the number of proteobacteria decreased in the CW–dig. sample (17.47%), but increased in the CW + PLA sample (39.82%) compared to the CW–control sample (32.70%). The analysis of biofilm formation dynamics using the BioFlux microfluidic system shows a significantly faster growth of the biofilm surface area for the CW + PLA sample. This information was complemented by observations of the morphological characteristics of the microorganisms using fluorescence microscopy. The images of the CW + PLA sample showed carrier sections covered with microbial consortia.
Additives Improving the Efficiency of Biogas Production as an Alternative Energy Source - A Review
Additives for anaerobic digestion (AD) can play a significant role in optimizing the process by increasing biogas production, stabilizing the system, and improving digestate quality. The role of additives largely boils down to, among others, enhancing direct interspecies electron transfer (DIET) between microbial communities, resulting in improved syntrophic interactions, adsorption of toxic substances that may inhibit microbial activity, improving microbial activity, and increasing process stability and accelerating the decomposition of complex organic materials, thereby increasing the rate of hydrolysis. Through the aforementioned action, additives can significantly affect AD performance. The function of these materials varies, from enhancing microbial activity to maintaining optimal conditions and protecting the system from inhibitors. The choice of additives should be carefully tailored to the specific needs and conditions of the digester to maximize benefits and ensure sustainability. In light of these considerations, this paper characterizes the most commonly used additives and their combinations based on a comprehensive review of recent scientific publications, including a report on the results of conducted studies. The publication features chapters that describe carbon-based conductive materials, metal oxide nanomaterials, trace metal, and biological additives, including enzymes and microorganisms. It concludes with the chapters summarising reports on various additives and discussing their functional properties, as well as advantages and disadvantages. The presented review is a substantive and concise analysis of the latest knowledge on additives for the AD process. The application of additives in AD is characterized by great potential; hence, the subject matter is very current and future-oriented.