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Operation and challenges of biogas technology: a fundamental overview
The modern world is facing a huge energy crisis related to the depletion of conventional energy sources. Therefore, obtaining energy from alternative sources is sparking increasing interest, expressed by both scientists and entrepreneurs. One such source is biogas, which has great potential to become, along with wind and solar energy, an important renewable energy source (RES). This paper presents the technical and practical aspects of biogas production (mainly agricultural) and extensively discusses the anaerobic digestion (AD) process. The global development of biogas plants and the operation of the most important types of biogas plants are also discussed. In the conclusion section, the benefits of biogas technology development are provided and explained, as well as the challenges and barriers hindering the intensification of biogas plant construction despite the potential and access to adequate resources and waste materials.
Efektywna stabilizacja pofermentu z biogazowni rolniczej z wykorzystaniem biowęgla i mikroorganizmów promujących wzrost roślin
Study of the Environmental Kuznets Curve in the EU27 Countries Taking into Account Socio-Economic Factors and GHG and PM Emissions.
The study of the relationship between human economic activity and the state of the environment in recent decades is reflected, among others, in the study of the environmental Kuznets curve (EKC) hypothesis. Numerous attempts have been made to examine the existence of the EKC by correlating various measures of environmental devastation with GDP per capita indicators. In this study, the aim and research gap were to compare and confirm/exclude the obtained results with the studies previously conducted by the authors, which referred to the analysis of the EKC in relation to general GHG emissions. In this analysis, GHG emissions per capita were assumed. In recent years, in the EU countries, more and more attention has been paid to research on the EKC focused on GHG emissions, and a certain research gap has been noticed in the direction of EKC analyses for PM2.5 and PM10. In the context of PM emissions, the very negative impact on human health should be emphasized. The authors decided to analyze the EKC hypothesis based on the current available data also in relation to PM emissions. In this study, a group of socio-economic variables in the form of GDP, gross electricity production, passenger cars, and population were used in relation to GHG, PM2.5, and PM10 emissions in the EU-27 countries. The study used multiple regression analysis to test the direction of the relationship between pollutant emissions and GDP. In the case of Germany, a negative correlation was obtained for GHG, PM2.5, PM10 emissions, and GDP. The EKC approach can be successfully used both in system awareness (qualitative) and quantitative studies to prepare scenarios of changes in greenhouse gas and PM emissions and to create strategic planning, manage resources, promote innovation, and in climate policy.
Quantitative and Qualitative Changes in the Genetic Diversity of Bacterial Communities in Anaerobic Bioreactors with the Diatomaceous Earth/Peat Cell Carrier
This paper analyses the impact of the diatomaceous earth/peat (DEP; 3:1) microbial carrier on changes in the bacterial microbiome and the development of biofilm in the anaerobic digestion (AD) of confectionery waste, combined with digested sewage sludge as inoculum. The physicochemical properties of the carrier material are presented, with particular focus on its morphological and dispersion characteristics, as well as adsorption and thermal properties. In this respect, the DEP system was found to be a suitable carrier for both mesophilic and thermophilic AD. The evaluation of quantitative and qualitative changes in the genetic diversity of bacterial communities, carried out using next-generation sequencing (NGS), showed that the material has a modifying effect on the bacterial microbiome. While Actinobacteria was the most abundant cluster in the WF-control sample (WF—waste wafers), Firmicutes was the dominant cluster in the digested samples without the carrier (WF-dig.; dig.—digested) and with the carrier (WF + DEP). The same was true for the count of Proteobacteria, which decreased twofold during biodegradation in favor of Synergistetes. The Syntrophomonas cluster was identified as the most abundant genus in the two samples, particularly in WF + DEP. This information was supplemented by observations of morphological features of microorganisms carried out using fluorescence microscopy. The biodegradation process itself had a significant impact on changes in the microbiome of samples taken from anaerobic bioreactors, reducing its biodiversity. As demonstrated by the results of this innovative method, namely the BioFlux microfluidic flow system, the decrease in the number of taxa in the digested samples and the addition of DEP contributed to the microbial adhesion in the microfluidic system and the formation of a stable biofilm.
The Problem of Weed Infestation of Agricultural Plantations vs. the Assumptions of the European Biodiversity Strategy
Meeting the nutritional needs of a dynamically developing global society is a major challenge. Despite the modernisation of agriculture, huge losses in the quality and quantity of crops occur each year, mainly due to weed species, which are the most important biotic limitation to agricultural production. Globally, approximately 1800 weed species cause a 31.5% reduction in plant production, which translates to USD 32 billion per year in economic losses. However, when the same herbicides are frequently applied, plants develop segetal immune mechanisms. There are currently around 380 herbicide-resistant weed biotypes worldwide. Due to the negative influence of herbicides on ecosystems and the legal regulations that limit the use of chemical crop protection products, it is necessary to develop a new method of weed control. Bioherbicides, based on living organisms or their secondary metabolites, seem to be an ideal solution. The biocontrol market is worth around EUR 550 million in Europe and EUR 1.6 billion worldwide, with an estimated 15% growth expected by 2025. Despite numerous studies that have demonstrated the effectiveness of microbial bioherbicides, only 25 mould-based bioherbicides are currently available to growers. Due to the high specificity and selectivity of biological crop protection products, as well as their low production costs and non-toxicity to the environment and human health, they would appear to be a safe alternative to chemical pesticides.
Nośniki w fermentacji metanowej bioodpadów: ujęcie procesowo–mikrobiologiczne na przykładzie wybranych układów
Biofilm Formation and Genetic Diversity of Microbial Communities in Anaerobic Batch Reactor with Polylactide (PLA) Addition
In this paper, an anaerobic digestion (AD) study was conducted on confectionery waste with granular polylactide (PLA) as a cell carrier. Digested sewage sludge (SS) served as the inoculum and buffering agent of systems. This article shows the results of the analyses of the key experimental properties of PLA, i.e., morphological characteristics of the microstructure, chemical composition and thermal stability of the biopolymer. The evaluation of quantitative and qualitative changes in the genetic diversity of bacterial communities, performed using the state-of-the-art next generation sequencing (NGS) technique, revealed that the material significantly enhanced bacterial proliferation; however, it does not change microbiome biodiversity, as also confirmed via statistical analysis. More intense microbial proliferation (compared to the control sample, without PLA and not digested, CW–control, CW–confectionery waste) may be indicative of the dual role of the biopolymer—support and medium. Actinobacteria (34.87%) were the most abundant cluster in the CW–control, while the most dominant cluster in digested samples was firmicutes: in the sample without the addition of the carrier (CW–dig.) it was 68.27%, and in the sample with the addition of the carrier (CW + PLA) it was only 26.45%, comparable to the control sample (CW–control)—19.45%. Interestingly, the number of proteobacteria decreased in the CW–dig. sample (17.47%), but increased in the CW + PLA sample (39.82%) compared to the CW–control sample (32.70%). The analysis of biofilm formation dynamics using the BioFlux microfluidic system shows a significantly faster growth of the biofilm surface area for the CW + PLA sample. This information was complemented by observations of the morphological characteristics of the microorganisms using fluorescence microscopy. The images of the CW + PLA sample showed carrier sections covered with microbial consortia.
Eco-Friendly and Effective Diatomaceous Earth/Peat (DEP) Microbial Carriers in the Anaerobic Biodegradation of Food Waste Products
This article aims to present the results of research on anaerobic digestion (AD) of waste wafers (WF-control) and co-substrate system—waste wafers and cheese (WFC-control), combined with digested sewage sludge. The aim of this study was to assess the physicochemical parameters of the diatomaceous earth/peat (DEP; 3:1) carrier material and to verify its impact on the enzymatic activity and the process performance. The experiment was conducted in a laboratory, in a periodical mode of operation of bioreactors, under mesophilic conditions. The results of analyses of morphological-dispersive, spectroscopic, adsorption, thermal, and microbiological properties confirmed that the tested carrier material can be an excellent option to implement in biotechnological processes, especially in anaerobic digestion. As part of the experiment, the substrates, feedstock, and fermenting slurry were subjected to the analysis for standard process parameters. Monitoring of the course of AD was performed by measuring the values of key parameters for the recognition of the stability of the process: pH, VFA/TA ratio (volatile fatty acids/total alkalinity), the content of NH4+, and dehydrogenase activity, as an indicator of the intensity of respiratory metabolism of microorganisms. No significant signals of destabilization of the AD process were registered. The highest dehydrogenase activity, in the course of the process, was maintained in the WFC + DEP system. The microbial carrier DEP, used for the first time in the anaerobic digestion, had a positive effect on the yield of methane production. As a result, an increase in the volume of produced biogas was obtained for samples fermented with DEP carrier material for WF + DEP by 13.18% to a cumulative methane yield of 411.04 m3 Mg−1 VS, while for WFC + DEP by 12.85% to 473.91 m3 Mg−1 VS.
An Agricultural Biogas Plant as a Thermodynamic System: A Study of Efficiency in the Transformation from Primary to Secondary Energy
Using a wide range of organic substrates in the methane fermentation process enables efficient biogas production. Nonetheless, in many cases, the efficiency of electricity generation in biogas plant cogeneration systems is much lower than expected, close to the calorific value of the applied feedstock. This paper analyses the energy conversion efficiency in a 1 MWel agricultural biogas plant fed with corn silage or vegetable waste and pig slurry as a feedstock dilution agent, depending on the season and availability. Biomass conversion studies were carried out for 12 months, during which substrate samples were taken once a month. The total primary energy in the substrates was estimated in laboratory conditions by measuring the released heat (17,760 MWh·year−1), and, in the case of pig slurry, biochemical methane potential (BMP, (201.88 ± 3.21 m3·Mg VS−1). Further, the substrates were analysed in terms of their chemical composition, from protein, sugar and fat content to mineral matter determination, among other things. The results obtained during the study were averaged. Based on such things as the volume of the biogas, the amount of chemical (secondary) energy contained in methane as a product of biomass conversion (10,633 MWh·year−1) was calculated. Considering the results obtained from the analyses, as well as the calculated values of the relevant parameters, the biomass conversion efficiency was determined as the ratio of the chemical energy in methane to the (primary) energy in the substrates, which was 59.87%, as well as the electricity production efficiency, as the ratio of the electricity produced (4913 MWh·year−1) to the primary energy, with a 35% cogeneration system efficiency. The full energy conversion efficiency, related to electricity production, reached a low value of 27.66%. This article provides an insightful, unique analysis of energy conversion in an active biogas plant as an open thermodynamic system.
The Role of Hydropower in Climate-Resilient Energy Systems: Case Study of the Jeziorsko Reservoir (Poland).
Methods of Handling the Cup Plant (Silphium perfoliatum L.) for Energy Production
The aim of the study was to determine the possibilities of using cup plants (Silphium perfoliatum L.) to generate energy. The energy balances of the combustion and anaerobic digestion were compared. The research showed that cup plants could be used as a raw material for solid fuel and for anaerobic digestion. An energy balance simulation showed that electricity could be generated through the anaerobic digestion of cup plants. The following amounts could be generated in the anaerobic digestion process: 1069 kWhe from 1 Mg of the raw material fragmented with an impact mill, 738.8 kWhe from 1 Mg of the raw material extruded at a temperature of 150 °C, and as much as 850.1 kWhe from 1 Mg of the raw material extruded at 175 °C. The energy balance of the combustion of biofuel in the form of cup plant pellets showed that 858.28 kWht could be generated from 1 Mg of the raw material. The combustion of solid biofuel generated a relatively low amount of heat in comparison with the expected amount of heat from a biogas-powered cogeneration system due to the high energy consumption of the processes of drying and agglomeration of the raw material for the production of pellets.
Bioenergy Generation from Different Types of Waste by Anaerobic Digestion
One of the problems of the modern world is the generation of increasing amounts of waste by agriculture and various industries [...]
Computational and experimental approaches to assessing the biochemical methane potential (BMP) of organic substrates for biogas plants
The Use of Fungi of the Trichoderma Genus in Anaerobic Digestion: A Review
Plant waste biomass is the most abundant renewable energy resource on Earth. The main problem with utilising this biomass in anaerobic digestion is the long and costly stage of degrading its complex structure into simple compounds. One of the promising solutions to this problem is the application of fungi of the Trichoderma genus, which show a high capacity to produce hydrolytic enzymes capable of degrading lignocellulosic biomass before anaerobic digestion. This article discusses the structure of plant waste biomass and the problems resulting from its structure in the digestion process. It presents the methods of pre-treatment of lignocellulose with a particular focus on biological solutions. Based on the latest research findings, key parameters related to the application of Trichoderma sp. as a pre-treatment method are discussed. In addition, the possibility of using the digestate from agricultural biogas plants as a carrier for the multiplication of the Trichoderma sp. fungi, which are widely used in many industries, is discussed.
Bioenergy from Maize Silage by Anaerobic Digestion: Batch Kinetics in Relation to Biochemical Composition
Maize silage can play a key role in policies aimed at stabilising local energy systems, as it constitutes a critical renewable feedstock for European biogas plants. By providing a dense and predictable source of chemical energy, it supports balance and reliability in the agricultural energy sector. To convert this potential into stable energy production, operators require kinetic models that translate routine silage quality indicators into concrete guidance for digester operation and control. Therefore, the aim of this article was to evaluate the batch kinetics of anaerobic digestion (AD) of maize silage and to select an adequate model for describing biochemical methane potential (BMP) profiles and associated energy recovery in the context of start-up, organic loading rate (OLR), hydraulic retention time (HRT) and feedstock preparation. Ten batches of silage (A–J) were examined, covering a realistic range of pH, electrical conductivity (EC), dry and volatile solids, ash, protein–fat–fibre fractions, fibre composition (NDF, ADF and ADL), derived fractions (hemicellulose, cellulose, and residual organic matter (OM)), C/N ratio and macro-/micronutrient profiles, including trace elements relevant to methanogenesis (Ni, Co, Mo, and Se). BMP tests were carried out in batch mode, and the resulting curves were fitted using the modified Gompertz and a first-order kinetic model. Methane yields of approx. 100–120 m3 CH4/Mg fresh matter (FM) and 336–402 m3 CH4/Mg volatile solids (VS), with CH4 contents of 52–57% v/v, were typical for energy-grade maize silage. Kinetic and energetic behaviours were governed mainly by residual OM and hemicellulose (shortening the lag phase and increasing the maximum methane production rate), the ADL/cellulose ratio (controlling the slower hydrolytic tail), EC and Na/Cl/S (extending the lag phase), and C/N together with Ni/Co/Mo/Se (stabilising methanogenesis). The modified Gompertz model reproduced BMP curves with a pronounced lag phase and asymmetry more accurately (lower error and better information criterion values), and its parameters directly support start-up design, OLR ramp-up and energetic performance optimisation in bioenergy reactors. The novelty of this work lies in combining batch BMP tests, comparative kinetic modelling and detailed silage characterisation to establish quantitative links between kinetic parameters and routine maize silage quality indicators that are directly relevant for biogas plant operation and renewable energy production.
