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Serum Proteomic Analysis in Obese Postmenopausal Women Before and After Multi-Species Probiotic Supplementation
The composition and functions of the gut microbiota can affect the development of obesity and metabolic disorders. Proteomics analysis has become important tool for characterizing the proteome and identifying serum biomarkers associated with specific conditions in different groups of patients. The aim of this study was to gain insight into the pathophysiological aspects of obesity and the relation between comorbidities and the gut microbiota by characterizing the protein profile in obese post-menopausal women before and after multi-species probiotic supplementation. MALDI-TOF MS analyses were performed in serum samples from the placebo group 24, from the low-dose probiotic group 24, and 23 from the high-dose probiotic group. Paired comparison analysis of protein–peptide patterns derived from patients at two time points (i.e., before and after treatment) revealed several peaks changing under treatment. Complement C3, Fibrinogen alpha chain, Coagulation factor XII, Tubulin beta-3 chain, Fibrinogen beta chain, Inter-alpha-trypsin inhibitor heavy chain H4, and Apolipoprotein A-I were identified. Depending on the administered dose, slightly different alterations were observed. In summary, the obtained results do not allow us to draw a conclusion regarding the influence of probiotic supplementation on peptide profile in obese post-menopausal women. However some of identified proteins could take part in the complex mechanism involving obesity, dysbiosis, and inflammation. Registration number: NCT03100162
Influence of supplementation of probiotic bacteria Lactobacillus plantarum and Lactobacillus curvatus on selected parameters of liver iron metabolism in rats on high-fat iron-deficient diet
Lactiplantibacillus plantarum, Duodenal Hydroxyphenyllactic Acid and Iron: Insights from a Rat Model of a High-Fat Iron-Deficient Diet
Influence of supplementation with iron and probiotic bacteria Lactobacillus plantarum and Lactobacillus curvatus on selected parameters of inflammatory state in rats on a high-fat iron-deficient diet
AbstractBACKGROUNDA high‐fat (HF) diet, diet iron deficiency and iron supplementation may affect inflammatory parameters. Probiotics influence both iron metabolism and inflammation. We compared the inflammatory state in rats on a HF iron‐deficient diet receiving oral iron, Lactobacillus plantarum and Lactobacillus curvatus in different combinations.METHODSThis was a two‐stage experiment. In groups C (n = 8) and HF (n = 8), rats ate a control or HF diet, respectively, for 16 weeks. In the group HFDEF (n = 48), rats ate a HF iron‐deficient diet for 8 weeks (first stage) and were subsequently divided into 6 groups (n = 8 each) receiving the following for a further 8 weeks (second stage): HFDEF – a HF iron‐deficient diet; HFDEFFe – a HF iron‐deficient diet with iron; HFDEFLp and HFDEFLc – a HF iron‐deficient diet with L. plantarum or L. curvatus, respectively; and HFDEFFeLp and HFDEFFeLc – a HF iron‐deficient diet with iron and L. plantarum or L. curvatus, respectively. Body composition analysis and blood sampling was performed. Markers of iron status and levels of total antioxidant status (TAS), C‐reactive protein (CRP), tumour necrosis factor alpha (TNF‐α) and interleukin 6 (IL‐6) were measured in the blood.RESULTSTAS was higher in the HFDEF group (756.57 ± 489.53 ng mL−1) versus the HFDEFLc group (187.04 ± 47.84 ng mL−1; P = 0.022). No more differences were found between groups, or in TAS, CRP, TNF‐α and IL‐6 concentrations. Also, no differences were found between groups for alanine and aspartate aminotransferases, glucose, total cholesterol, low‐ and high‐density lipoproteins and triglycerides. TAS level was positively correlated with ferritin concentration, IL‐6 with TAS and TNF‐α with hepcidin level.CONCLUSIONSSupplementation with L. plantarum, L. curvatus and iron in combinations exerts no influence on inflammatory status, lipid profile, hepatic function and serum fasting glucose in rats on a HF iron‐deficient diet. © 2024 Society of Chemical Industry.