2025, Cholik, Rafsan Syabani, Skrypnik, Katarzyna, Karaźniewicz-Łada, Marta, Waśkiewicz, Agnieszka, Suliburska, Joanna

2025, Skrypnik, Damian, Skrypnik, Katarzyna, Granell, José Casaña, Woszczyk, Dawid, Suliburska, Joanna

Abstract The role of myokines as a link between cardiac rehabilitation (CR) and cardiovascular benefits in patients recovering from acute coronary syndrome (ACS) is important but not well understood. We investigated the effect of CR on circulating levels of myostatin, follistatin, apelin, and follistatin-related protein 1 (FSTL1) in post-ACS patients. A total of 110 patients underwent a 2-week CR programme (group S) and were compared with 110 non-CR patients (group K). In group S, blood pressure, heart rate, anthropometrics, body composition, and serum myokine levels were measured at baseline and post-CR; in group K, these were assessed once. After CR, apelin, myostatin, and FSTL1 increased in group S. Apelin and myostatin were higher in group S post-CR compared with group K, while follistatin remained higher in group K at both time points. FSTL1 was initially higher in group K but increased post-CR in STEMI patients only; myostatin increased in NSTEMI patients. Apelin increased in STEMI patients. Apelin and myostatin levels were independent of cardiovascular risk factors. Post-CR, follistatin correlated inversely with diastolic pressure; FSTL1 was related to fat tissue, muscle mass and body mass index. CR modulates key myokines and differential myokine responses in STEMI vs. NSTEMI patients support the need for personalised rehabilitation strategies. ClinicalTrials.gov registration number: NCT03935438.

2025, Skrypnik, Katarzyna, Selvan, Damla, Suliburska, Joanna

2024, Harahap, Iskandar Azmy, Moszak, Małgorzata, Człapka-Matyasik, Magdalena, Skrypnik, Katarzyna, Bogdański, Paweł, Suliburska, Joanna

BackgroundMenopause poses significant health risks for women, particularly an increased vulnerability to fractures associated with osteoporosis. Dietary interventions have emerged as promising strategies, focusing on mitigating the risk of osteoporosis rather than solely addressing the established disease. This 12-week randomized controlled trial aimed to analyze the effects of consuming Lactobacillus acidophilus probiotics on calcium levels, biomarkers of bone metabolism, and bone mineral density (BMD) profiles in postmenopausal women.MethodsFifty-five participants were randomly assigned to receive either a placebo (n = 25) or the probiotic L. acidophilus UALa-01™ (n = 30) daily via oral intervention. Throughout the study, evaluations included body composition, blood biochemical parameters, serum calcium levels, and biomarkers of bone metabolism. Additionally, Dual-energy X-ray absorptiometry was used to measure BMD profiles.ResultsThe findings delineated that the probiotic group experienced a decrease in serum calcium levels compared to their initial levels. However, hair calcium levels and biomarkers related to bone metabolism showed no notable changes within this group. Consumption of probiotic L. acidophilus also seemed to prevent fluctuations in bone turnover markers. Moreover, there were no significant alterations in BMD levels at the lumbar spine, left femur, and total body in the probiotic group. Additionally, probiotic intake led to favorable outcomes by significantly reducing both body fat and visceral fat during the intervention period. Conversely, an adverse effect of consuming probiotic L. acidophilus was observed with a significant increase in glucose concentration.ConclusionIn conclusion, the consumption of L. acidophilus probiotics daily for 12 weeks among postmenopausal women does not affect the profile of BMD, but it may help in stabilizing bone turnover. It is important to note that most measured parameters were within the normal range for this population. However, it is worth noting that 3 months of probiotic supplementation could potentially disrupt calcium and glucose status in postmenopausal women.

2023, Skrypnik, Damian, Skrypnik, Katarzyna, Suliburska, Joanna, Bogdański, Paweł

SummaryBy 2030, it is expected that a billion people will have suffer from obesity. Adipose tissue synthesizes leptin, an adipokine that affects cardiovascular risk. Leptin intensifies the synthesis of vascular endothelial growth factor (VEGF). Our study reviews recent reports on leptin–VEGF crosstalk in obesity and related disorders. PubMed, Web of Science, Scopus, and Google Scholar were searched. One hundred and one articles involving human, animal, and in vitro research were included. In vitro studies show the crucial role of interaction between endothelial cells and adipocytes and hypoxia as a factor that intensifies leptin's effects on VEGF. Leptin–VEGF crosstalk promotes the progression of cancer. The animal research reveal that a high‐fat diet enhances leptin and VEGF crosstalk. Genetic and epigenetic mechanisms and procreator‐offspring programming may be involved in leptin–VEGF crosstalk. Some female‐specific characteristics of leptin–VEGF relation in obesity were observed. The human studies have shown that increased leptin and VEGF synthesis and leptin–VEGF crosstalk are factors linking obesity with elevated cardiovascular risk. The studies of the last 10 years documented a range of significant aspects of leptin–VEGF crosstalk specific for obesity and related disorders, shedding new light on the link between obesity and increased cardiovascular risk.

2025, Skrypnik, Katarzyna, Olejnik-Schmidt, Agnieszka, Mikołajczyk-Stecyna, Joanna, Schmidt, Marcin, Suliburska, Joanna

2025, Cholik, Rafsan Syabani, Skrypnik, Katarzyna, Waśkiewicz, Agnieszka, Karaźniewicz-Łada, Marta, Suliburska, Joanna

Abstract Low-dose aspirin prophylaxis is recommended for women at high risk of preeclampsia. It has been suggested that dietary salicylates may have a similar effect. Despite the known anti-inflammatory properties of salicylates, their influence on trace elements in preeclampsia remains unclear. This research investigated the effect of dietary salicylates and aspirin on iron, zinc, and copper status in rats with NG-nitro-L-arginine methyl ester (L-NAME)–induced preeclampsia. The study involved pregnant Sprague Dawley rats divided into six groups: control group (CH), preeclamptic rats (CP), preeclamptic rats with a low dose of dietary salicylate (LSP), preeclamptic rats with a high dose of dietary salicylate, preeclamptic rats with a low dose of aspirin (LAP), and preeclamptic rats with a high dose of aspirin. The content of trace elements in diets, liver, kidney, heart, spleen, pancreas, femur, brain, and hair was measured using flame atomic absorption spectrometry. Salicylate concentrations in diets, serum, and urine were analyzed using HPLC and UHPLC-MS/MS systems. Administration of L-NAME resulted in elevated blood pressure across groups, and only the LAP group had blood pressure levels comparable to the CH group. Preeclampsia significantly decreased serum hepcidin levels, while salicylates abolished this effect. Salicylate administration significantly decreased iron levels in hair and increased maternal zinc concentrations in the brain. Dietary salicylates markedly increased zinc levels in the placenta. In conclusion, L-NAME–induced preeclampsia decreases maternal serum hepcidin. Treatment with salicylates modulates iron and zinc status in preeclamptic rats, with specific effects on hepcidin levels.

2024, Skrypnik, Katarzyna, Schmidt, Marcin, Olejnik-Schmidt, Agnieszka, Harahap, Iskandar Azmy, Suliburska, Joanna

AbstractBACKGROUNDA high‐fat (HF) diet, diet iron deficiency and iron supplementation may affect inflammatory parameters. Probiotics influence both iron metabolism and inflammation. We compared the inflammatory state in rats on a HF iron‐deficient diet receiving oral iron, Lactobacillus plantarum and Lactobacillus curvatus in different combinations.METHODSThis was a two‐stage experiment. In groups C (n = 8) and HF (n = 8), rats ate a control or HF diet, respectively, for 16 weeks. In the group HFDEF (n = 48), rats ate a HF iron‐deficient diet for 8 weeks (first stage) and were subsequently divided into 6 groups (n = 8 each) receiving the following for a further 8 weeks (second stage): HFDEF – a HF iron‐deficient diet; HFDEFFe – a HF iron‐deficient diet with iron; HFDEFLp and HFDEFLc – a HF iron‐deficient diet with L. plantarum or L. curvatus, respectively; and HFDEFFeLp and HFDEFFeLc – a HF iron‐deficient diet with iron and L. plantarum or L. curvatus, respectively. Body composition analysis and blood sampling was performed. Markers of iron status and levels of total antioxidant status (TAS), C‐reactive protein (CRP), tumour necrosis factor alpha (TNF‐α) and interleukin 6 (IL‐6) were measured in the blood.RESULTSTAS was higher in the HFDEF group (756.57 ± 489.53 ng mL−1) versus the HFDEFLc group (187.04 ± 47.84 ng mL−1; P = 0.022). No more differences were found between groups, or in TAS, CRP, TNF‐α and IL‐6 concentrations. Also, no differences were found between groups for alanine and aspartate aminotransferases, glucose, total cholesterol, low‐ and high‐density lipoproteins and triglycerides. TAS level was positively correlated with ferritin concentration, IL‐6 with TAS and TNF‐α with hepcidin level.CONCLUSIONSSupplementation with L. plantarum, L. curvatus and iron in combinations exerts no influence on inflammatory status, lipid profile, hepatic function and serum fasting glucose in rats on a HF iron‐deficient diet. © 2024 Society of Chemical Industry.

2025, Harahap, Iskandar Azmy, Skrypnik, Katarzyna, Kołodziejski, Paweł, Suliburska, Joanna

2022, Wesolek, A., Skoracka, K., Skrypnik, Katarzyna, Suliburska, Joanna, Bogdanski, P., Szulinska, M., Skrypnik, D.

2022, Skrypnik, Damian, Skrypnik, Katarzyna, Suliburska, Joanna, Bogdański, Paweł

AbstractLeptin, a well-proven cardiovascular risk factor, influences vascular endothelial growth factor A (VEGF A) synthesis via hypoxia-inducible factor 1 alpha (HIF-1A), nuclear factor kappa-light-chain-enhancer of activated B cells (NfkB) and NILCO (Notch, interleukin 1 [IL1] and leptin cross-talk outcome) pathways. This study aimed to investigate the influence of cardiac rehabilitation (CR) on HIF-1A, NfkB and NILCO dependent leptin and VEGF A cross-talk in patients after acute coronary syndrome (ACS). Fifty post-ACS patients underwent a 2-week CR programme (study group S) and were compared to 50 post-ACS subjects who did not undergo CR (control group K). In group S, at baseline and at completion and in group K once, anthropometric, body composition, blood pressure and heart rate measurements were taken and blood sampling was performed. Serum levels of leptin, VEGF A, VEGF receptor 2 (VEGF R2), HIF-1A, NfkB, interleukin 1-alpha (IL1-alpha) and Notch 1 were determined. In group S, serum VEGF A levels increased while leptin, HIF-1A and VEGF R2 levels decreased and completion but not baseline serum leptin correlated positively with serum VEGF A. Also, serum completion VEGF A correlated positively with NfkB and HIF-1A in group S. Correlation analysis in group S confirmed the significant role of the NILCO pathway in the regulation of VEGF A serum levels mediated by HIF-1A and NfkB. CR may induce the predomination of the NILCO pathway interacting with HIF-1A and NfkB over leptin canonical and non-canonical signalling pathways in the leptin influence on VEGF A in post-ACS patients.Trial registration: ClinicalTrials.gov ID: NCT03935438. The CARDIO-REH randomised study.

2022, Skrypnik, Katarzyna, Olejnik-Schmidt, Agnieszka, Mikołajczyk-Stecyna, Joanna, Schmidt, Marcin, Suliburska, Joanna

2025, Kręgielska-Narożna, Matylda, Miller-Kasprzak, Ewa, Matuszewska, Eliza, Matysiak, Jan, Skrypnik, Katarzyna, Łoniewski, Igor, Szulińska, Monika, Bogdański, Paweł

The composition and functions of the gut microbiota can affect the development of obesity and metabolic disorders. Proteomics analysis has become important tool for characterizing the proteome and identifying serum biomarkers associated with specific conditions in different groups of patients. The aim of this study was to gain insight into the pathophysiological aspects of obesity and the relation between comorbidities and the gut microbiota by characterizing the protein profile in obese post-menopausal women before and after multi-species probiotic supplementation. MALDI-TOF MS analyses were performed in serum samples from the placebo group 24, from the low-dose probiotic group 24, and 23 from the high-dose probiotic group. Paired comparison analysis of protein–peptide patterns derived from patients at two time points (i.e., before and after treatment) revealed several peaks changing under treatment. Complement C3, Fibrinogen alpha chain, Coagulation factor XII, Tubulin beta-3 chain, Fibrinogen beta chain, Inter-alpha-trypsin inhibitor heavy chain H4, and Apolipoprotein A-I were identified. Depending on the administered dose, slightly different alterations were observed. In summary, the obtained results do not allow us to draw a conclusion regarding the influence of probiotic supplementation on peptide profile in obese post-menopausal women. However some of identified proteins could take part in the complex mechanism involving obesity, dysbiosis, and inflammation. Registration number: NCT03100162

2025, Skoracka, K., Skrypnik, D., Seraszek-Jaros, A., Skrypnik, Katarzyna, Suliburska, Joanna, Szulinska, M., Bogdanski, P.

2022, Skrypnik, Katarzyna, Bogdański, Paweł, Sobieska, Magdalena, Schmidt, Marcin, Suliburska, Joanna

2025, Suliburska, Joanna, Cholik, Rafsan Syabani, Karaźniewicz-Łada, Marta, Wronka, Dorota, Karlik, Anna, Waśkiewicz, Agnieszka, Skrypnik, Katarzyna, Kołodziejski, Paweł, Cieślak, Adam, Przybył, Łukasz

Background The pathophysiology of preeclampsia involves impaired cytotrophoblastic invasion, placental ischemia, inflammation, and angiogenic imbalance. Prophylactic low-dose aspirin can reduce the risk of preeclampsia and fetal growth restriction in high-risk women. This study evaluated the effect of dietary salicylates on the development of preeclampsia in rats treated with L-NAME (NG-nitro-L-arginine-methyl ester). Methodology Pregnant Sprague-Dawley rats were randomly assigned to six groups and treated with dietary salicylates at two dose levels (1 and 10 mg/kg diet) or aspirin (doses adjusted to dietary salicylates). Preeclampsia was induced by administering L-NAME in drinking water from gestational days 6–19. Results Neither dietary salicylates nor aspirin, at either dose, affected blood pressure in L-NAME-treated rats. The lower dose of dietary salicylates significantly reduced urinary albumin levels. Both interventions prevented an increase in the sFlt/PLGF ratio and mitigated histopathological placental changes in preeclamptic rats. The higher dose of aspirin reduced placental VEGFR2 protein levels. Conclusion Dietary salicylate supplementation does not provide clear preventive effects against preeclampsia.

2024, Skrypnik, Katarzyna, Pluta, Dagmara, Wojtowicz, Mariusz, Rhaiem, Tahar Ben, Suliburska, Joanna

2023, Skrypnik, Katarzyna

2026, Selvan, D., Skrypnik, Katarzyna, Suliburska, Joanna