Histogenesis of the Uterine Horn in the Domestic Cat (Felis silvestris catus): LM, TEM, and SEM Study

This study employs light microscopy, scanning electron microscopy, and transmission electron microscopy to describe the morphological changes occurring during the development of the domestic cat’s uterine horns, originating from the uterine segments of paramesonephric ducts (uPD). Comprehensive observations conducted on 60 specimens aged 28–63 days post-conception (p.c.) revealed that the formation of the endometrium and myometrium in the uterine horns begins around day 33 p.c., initiated by mesenchymal differentiation. During endometrial development, fibroblasts align first in perpendicular and then in oblique columns. The subdivision of the lamina propria into basal and functional layers becomes evident shortly before birth, with the functional layer remaining flat until the end of the prenatal period. The endometrial epithelium transforms from a simple columnar to a pseudostratified structure, undulating by day 63 p.c. Myometrial formation commences with the differentiation of myoblasts, which are arranged in a circular pattern. By the end of gestation, these myoblasts differentiate into smooth muscle cells, organizing into distinct inner circular and outer longitudinal sublayers. Although the fundamental layered architecture of the uterine wall is established before birth, its full maturation—including gland formation, epithelial transformation, and further development of the myometrium - continues postnatally.