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Cerebral Vascularization and the Remaining Area Supply of the Internal Carotid Artery Derivatives of the Red Kangaroo (Osphranter rufus)
The red kangaroo (Osphranter rufus) is a member of Macropidideae superfamily. It is one of the four kangaroo species living nowadays, and it is the biggest one. It is native to Australia, where it is an abundant species living across the whole continent in stable populations. Outside its natural habit, the red kangaroo is a common species found in zoos and as patients in wildlife rehabilitation centers. Reports on kangaroo anatomy are scarce. Describing detailed anatomy is a base for establishing diagnostic and treatment protocols for different species of animals. Cardiovascular diseases and pathological changes suggestive of hypertension have been previously described in kangaroos. This creates a necessity for detailed studies on species’ vascular anatomy. New reports in the field of detailed vascular anatomy can bring considerable information that complements numerous studies on the evolution or biology of individual species. In this article, we describe the arterial vascularization of the brain and nearby regions of the cranial cavity using various anatomical techniques. The vascularization of the brain is discussed and compared with different mammalian species.
Effects of raw and fermented rapeseed cake on ruminal fermentation, methane emission, and milk production in lactating dairy cows
Thyroid ultrasound scanning technique in dogs and cats
Abstract Objective To demonstrate the ultrasound scanning technique of the thyroid gland in dogs and cats. Animals Dogs and cats undergoing thyroid ultrasonography. Methods Patients are typically positioned in dorsal recumbency; however, the examination can also be performed in other positions. A standing position may be particularly beneficial in cats by gently stabilizing the head with one hand while holding the probe in the other hand. The ventral cervical region is clipped, and alcohol is applied, followed by a coupling gel. A high-frequency linear probe is placed transversely over the trachea, caudal to the larynx. The probe is slowly moved caudally until the thyroid lobes are identified lateral to the trachea and medial to the carotid arteries. Each lobe is assessed in transverse and longitudinal planes. The parathyroid glands, when visible, are noted. Cine loops are recorded when necessary to assist in image review. Results The technique consistently allows identification and evaluation of the thyroid lobes in both dogs and cats. In most cases, examinations are successfully performed without sedation. Clinical Relevance Ultrasonography provides a noninvasive and accessible method for evaluating the thyroid glands in dogs and cats. The mastery of proper scanning techniques facilitates the recognition of anatomical structures and pathological changes, enhancing both clinical and research applications. This method also provides a foundation for ultrasound-guided sampling procedures.
Seroprevalence of Toxoplasma gondii in domestic cats, dogs and rabbits from Poland
AbstractThe seroprevalence of Toxoplasma gondii in domestic cats, dogs and rabbits was evaluated. Samples from cats and dogs were collected from five veterinary practices from various parts of Poland - Poznan (wielkopolskie voivodeship), Przemysl (podkarpackie voivodeship), Kluczbork (opolskie voivodeship), Lublin (lubelskie voivodeship) and Deblin (lubelskie voivodeship). Moreover, the samples from rabbits were collected in Poznan. In total, serum samples from 193 cats, 204 dogs and 71 rabbits were randomly selected and tested for specific antibodies against T. gondii using a commercial ELISA test. Pathogen seroprevalence among cats and dogs was calculated at a 95% confidence interval (CI) for each sex and age category (up to 12 months, 1–3 years, 4–7 years and over 8 years) and compared with a chi-squared test. The highest seroprevalence of T. gondii was noted in cats − 49.74% (96/193; 95% CI: 42.76–56.73). In dogs, it reached 28.92% (59/204; 95% CI: 23.13–35.49). Only 1 rabbit (3-year-old male) was seropositive, and the seroprevalence in rabbits was 1.41% (1/71; 95% CI: 0.25–7.56). A statistically significant correlation between seropositivity and age (p < 0.05) was observed in cats and dogs. No statistically significant difference in seroprevalence concerning gender or location was found in cats and dogs. Our findings indicate that cat and dog serum samples had a high frequency of anti-T. gondii antibodies, while rabbit serum samples had low frequency and that these species are exposed to T. gondii in Poland and develop humoral response due to infection.
From Waste to Feed: Enhancing Sustainability in Ruminant Nutrition Through Cashew Apple Utilization – A Review
Abstract Growing global demand for livestock products has intensified the need for sustainable feed resources in ruminant nutrition, particularly in developing regions. With feed costs comprising up to 75% of production expenses, exploring alternative feed sources can offer both economic and environmental benefits. This study explored the potential of cashew apple (CA) as a novel and sustainable by-product for ruminants by reviewing relevant articles from databases such as Scopus, Web of Science, PubMed, and Google Scholar. CA has an average proximate composition (dry matter basis) of 130 g/kg crude protein, 71.7 g/kg crude fibre, 61.2 g/kg ether extract, 49.3 g/kg ash, 640 g/kg nitrogen free extract, and 2973 kcal/kg metabolizable energy, making it a valuable addition to ruminant diets. It can reduce reliance on traditional and high-cost feed sources and mitigate environmental pollution by reducing waste and contributing to circular agricultural practices. However, limitations such as high moisture content in fresh cashew fruit, seasonal availability, and rapid spoilage necessitate effective processing methods. There is a knowledge gap on the use of CA in ruminant diets, and there is a need to assess its impact on ruminal pH, short-chain fatty acids production, ammonia production, other rumen fermentation profiles, and animal performance. Based on the quantitative data of this study about the proximate composition of CA and some animal studies, it could be a viable and eco-friendly supplement to the ruminant diets. Addressing its storage and preservation challenges can unlock its potential in diversified, eco-friendly ruminant systems.
Cerebral arterial vascularization of the scimitar-horned oryx (Oryx dammah)
AbstractThe Scimitar-horned Oryx (Oryx dammah) is a large terrestrial mammal native to Africa. Since the year 2000, it is classified as extinct in the wild. It is a subject of various conservation projects. The aim of this study was to describe the arterial vascularization of the brain in this species of oryx. Three different anatomical methods were used to obtain a complete arterial pattern - latex injection, corrosion cast, and computed tomography. The arterial vascularization of the brain was described. The main components of the cerebral arterial circle were the rostral cerebral arteries and the caudal communicating arteries. These vessels were created from the intracranial part of the internal carotid artery, that emerged from the rostral epidural rete mirabile. In the juvenile specimen, the whole internal carotid artery was observed. The anatomical pathways of the blood supply to the brain are important during medical procedures in cases of congestion and fainting caused by inadequate brain blood perfusion.
Raw diets for dogs and cats: Potential health benefits and threats
Raw meat-based diets for pet nutrition are becoming increasingly popular. The percentage of meat content, composition of nutrients, and amount of additives started to play an important role in the recipe of a given food. However, the use of healthier and unprocessed food must also be balanced with the animal’s specific needs based on its anatomy, physiology, and behavior. There are many potential advantages and disadvantages of a biologically appropriate raw food (BARF) diet, and all of them should be considered before switching to this approach. Raw meat is considered a diet closest to nature and least processed. However, raw diets threaten pet health because of the potential for nutrient imbalances. The choice of raw meat in pets’ everyday diet should be supported by the veterinarian’s medical decision and preferably also with nutritionist help. Growing animals require a specific Ca:P ratio in their diet, which may be improper in raw meat. For cats, taurine levels must be carefully checked. In addition, an imbalanced raw-meat diet can be the cause of poor semen quality in males. Females are prone to inhibition of the estrus cycle, especially due to hyperthyroidism. Exogenous thyroid hormone intake is a real concern when feeding a neck/head meat with thyroid glands. There is also a possibility of bacterial or parasitic presence in raw meat. The present paper aims to summarize the current state of knowledge about the benefits and threats of eating a raw meat diet for the health concerns of companion animals.
First detection of Hedgehog coronavirus 1 in Poland
AbstractHedgehogs are common in the majority of European countries and are known to host various pathogens, including viruses. The recent discovery of MERS-related coronaviruses (CoVs) in hedgehogs from Germany, France, the UK, China, and Italy suggests that hedgehogs may represent a wild reservoir of betacoronaviruses. This study reports the first detection and characterization of novel betacoronovirus, subgenus Merbecovirus in wild hedgehogs in Poland. The CoV RNA was detected in 10 out of 40 hedgehogs’ rectal swabs and in 1 out of 18 samples of the lung. No viral RNA was identified in the duodenum and kidney. There was no significant relationship between clinical status, gender, hedgehogs' age, and coronaviral RNA detection. Phylogenetic analysis showed that CoVs detected in our study grouped together with other representatives of Hedgehog coronavirus 1 species identified in Western Europe. Our findings provide further evidence that hedgehogs are a natural reservoir of Merbecovirus. Considering the high mutation rate of CoVs and their potential for crossing interspecies barriers, the proper management of hedgehogs admitted to wildlife rehabilitation centres is needed. It cannot be excluded that merbecovirus strains detected in hedgehogs may recombine with other CoVs leading to new viruses with potential for interspecies transmission.
Profile of Selected Mineral Elements in Tibiotarsal Bone of the White-Tailed Sea Eagle in Its Natural Habitat
Mineral bone composition (dry matter, bones ash, P, Ca, Zn, Mn, Mg, and Cu) and Pb levels of tibiotarsi of seven White-Tailed Sea Eagles were assessed. Lead intoxication in different bird species including waterfowl and raptors is being studied worldwide. The bones were analyzed for Pb by mass spectrometry with excitation in inductively coupled plasma (ICP-MS Elan DRC II) and for bone composition by Atomic Emission Spectrometer (Agilent 4100 Microwave Plasma). Pb levels ranging from 3.54 µg/g to 74.6 µg/g DM suggest that some of the investigated birds might have been intoxicated by Pb. Results of this analysis were divided into two groups of bones, with bone Pb levels higher and lower than Pb toxicity levels, and mineral bone compositions of both groups were compared. The present study shows the differentiation of bone mineral composition among seven examined White-Tailed Sea Eagles, considered a specific species in raptors. Pb intoxication may not have a major influence on mineral bone composition in raptors. It also suggests that assessing bone composition of raptor bones may help finding the possible cause of their deaths.
Expression of adropin in the reproductive organs of healthy female dogs and those with cystic endometrial hyperplasia, pyometra, and ovarian cysts
Arterial Circle of the Brain of the Red-Necked Wallaby (Notamacropus rufogriseus)
The red-necked wallaby is a medium-sized marsupial species, which have increasingly been kept as pets around the world. In the study, the arterial blood supply for the brain in this species was described. The study was conducted on 50 specimens with two preparation methods. The main artery supplying the brain was the internal carotid artery. The arterial circle of the brain was closed from the caudal side. The anatomy of the arteries of the described region was compared with other groups of mammals. This is the first description of this anatomical area that has been carried out in a marsupial species. Understanding the anatomy of the circulatory system in the wallaby can be valuable for further physiological and pathophysiological studies.
Protective Action of Cannabidiol on Tiamulin Toxicity in Humans—In Vitro Study
The growing awareness and need to protect public health, including food safety, require a thorough study of the mechanism of action of veterinary drugs in consumers to reduce their negative impact on humans. Inappropriate use of veterinary drugs in animal husbandry, such as tiamulin, leads to the appearance of residues in edible animal tissues. The use of natural substances of plant origin, extracted from hemp (Cannabis sativa L.), such as cannabidiol (CBD), is one of the solutions to minimize the negative effects of tiamulin. This study aimed to determine the effect of CBD on the cytotoxicity of tiamulin in humans. The cytotoxic activity of tiamulin and the effect of its mixtures with CBD were tested after 72 h exposure to three human cell lines: SH-SY5Y, HepG2 and HEK-293. Cytotoxic concentrations (IC50) of the tested drug and in combination with CBD were assessed using five biochemical endpoints: mitochondrial and lysosomal activity, proliferation, cell membrane integrity and effects on DNA synthesis. Oxidative stress, cell death and cellular morphology were also assessed. The nature of the interaction between the veterinary drug and CBD was assessed using the combination index. The long-term effect of tiamulin inhibited lysosomal (SH-SY5SY) and mitochondrial (HepG2) activity and DNA synthesis (HEK-293). IC50 values for tiamulin ranged from 2.1 to >200 µg/mL (SH-SY5SY), 13.9 to 39.5 µg/mL (HepG2) and 8.5 to 76.9 µg/mL (HEK-293). IC50 values for the drug/CBD mixtures were higher. Reduced levels of oxidative stress, apoptosis and changes in cell morphology were demonstrated after exposure to the mixtures. Interactions between the veterinary drug and CBD showed a concentration-dependent nature of tiamulin in cell culture, ranging from antagonistic (low concentrations) to synergistic effects at high drug concentrations. The increased risk to human health associated with the presence of the veterinary drug in food products and the protective nature of CBD use underline the importance of these studies in food toxicology and require further investigation.
Isolation method and characterization of adipocytes as a tool for equine obesity research – In vitro study
Whole genome characteristics of hedgehog coronaviruses from Poland and analysis of the evolution of the Spike protein for its interspecies transmission potential
Abstract Background The hedgehogs have been recently identified as possible reservoir of Middle East respiratory syndrome coronavirus like (MERS-CoV-like). These viruses were classified as a distinct Betacoronavirus erinacei (BCoV-Eri) species within the MerBCoV-Eriirus subgenus. As coronaviruses are known for their ability to jump between different hosts, including humans, this can pose a particular threat to people in direct contact with hedgehogs, such as those working at animal asylums. Our previous studies have shown the presence of BCoV-Eri strains in animals collected in the wildlife rehabilitation centre. This study aimed to investigate the presence of CoV in subsequent hedgehogs collected from the urban area of Poland and their molecular characteristics. Results Monitoring for the presence of coronavirus infection in hedgehogs revealed five positive individuals. The presence of BCoV-Eri was found in a total of 20% of animals tested. Our analyses revealed no correlation between CoVs positivity and animal health conditions but a higher probability of such infection in juveniles and females. The whole genome of two Polish Hedgehog coronavirus 1 strains were sequenced and compared with available counterparts from European and Asian countries. Phylogenetic analysis showed that both CoV strains formed common cluster with other similar MerBCoV-Eriirus, but they were also found to be genetically variable and most changes in the S protein were identified. Our analysis revealed that some S protein sites of the Hedgehog coronavirus 1 strains evolved under positive selection pressure and of five such sites, three are in the S1 region while the other two in the S2 region of the Spike. Conclusions BCoV-Eri is to some extent prevalent in wildlife asylums in Poland. Given that the S protein of BCoVs-Eri is highly variable and that some sites of this protein evolve under positive selection pressure, these strains could potentially acquire a favourable feature for cross-species transmission. Consequently, the threat to humans working in such asylums is particularly high. Adequate biosecurity safeguards, but also human awareness of such risks, are therefore essential.
Cytogenetic and molecular insight into the genetic background of disorders of sex development in seventeen cats
AbstractThe genetic background of feline disorders of sex development (DSDs) is poorly understood. We performed comprehensive cytogenetic, molecular, and histological studies of 17 cats with abnormal external genitalia, unusual behavior, or tricolor coats (atypical in males). The DSD phenotype of three cats was associated with sex chromosome abnormalities: X/Y translocation (38,XXSRY+), 37,X/38,XY mosaicism, and XX/XY leukocyte chimerism. The remaining 14 affected cats were classified as XY DSD (SRY-positive). In this group and 38 normal males, we analyzed a priori selected candidate genes (SRY, TAC3, CYP11B1 and LHCGR). Only a previously reported nonpathogenic variant was found in SRY. Moreover, SRY gene copy number was determined, and three variants were observed: 6, 5 (modal), and 4 copies in a single DSD case. The known variants in TAC3 and CYP11B1, responsible for testicular hypoplasia, persistent primary dentition or congenital adrenal hyperplasia, were not found in the study group. Nine novel polymorphisms were identified in the LHCGR gene, one of which, a potentially regulatory indel variant in 5′UTR, was significantly associated (p = 0.0467) with XY DSD. Our report confirmed that abnormalities of sex chromosomes are important causes of feline DSDs. We also showed that the indel variant of LHCGR can be considered a promising marker associated with XY DSD phenotype.
Arterial Blood Supply to the Cerebral Arterial Circle in the Selected Species of Carnivora Order from Poland
Carnivores are a wide, diverse group of mammals whose representatives live all over the world. The study presents the results of the analysis of the arterial vascularization of the blood supply to the cerebral arterial circle of selected species in the Caniformia suborder living in Poland. The selected group consists of wild and farm animals—105 animals in total. Three different methods were used—latex preparation, corrosion cast, and cone-beam computed tomography angiography. The main source of blood for encephalon in the described species is the internal carotid artery, and the second one is the vertebral artery. The results were discussed in relation to the current knowledge of this field of research. Information on the potential physiological meaning of such vascular pattern has been provided.
The Arterial Circle of the Brain in the Bawean Deer (Axis kuhlii)
The Bawean deer (Axis kuhlii) is a small deer species endemic to the island of Bawean in Indonesia. The species is listed as critically endangered by the IUCN Red List. The current population is assessed to be less than 500 adults living in the wild. The cerebral arterial circle (also called the circle of Willis) is an anastomosis of arteries that supply the brain. The aim of this study was to describe the arterial vascularization of the brain in this species. Three different methods were used to obtain a complete arterial pattern of this region—latex injection, corrosion cast, and contrast-enhanced computed tomography. The arterial vascularization of the brain was described. The pattern of the arterial vessels supplying the brain in this species was similar to that described previously by ruminants. The vessel with the biggest lumen branching off from the circle of Willis was the medial cerebral artery. The basilar artery is a vessel with a narrow lumen and does not mediate the delivery of blood to the encephalon from the caudal side. The results of the study may be useful in creating veterinary protocols for treating vascular diseases in this species and further studies from the field of pathophysiology or pathology.
Rice Bran in Old Horses Nutrition and its Influence on Condition, Blood Biochemical Parameters, Total Feces Bacteria and Methanogen Population
Abstract This study aimed to verify whether the inclusion of 0.5 kg full-fat rice bran per day in the diet of geriatric horses will improve their condition, increase the population of methanogens in the cecum, and thus affect the biochemical blood parameters. The experiment included 2 research periods: 6 healthy, non-working horses over 20 years of age (480 ± 20 kg of body weight) fed only hay (±8.86 kg/day/head) in the first period and hay (±8.00 kg/day/head) and rice bran (0.5 kg/day/head) in the second one. Each of these periods lasted 4 months. The Body Condition Scoring (BCS) assessment was performed at the beginning and end of the experiment. Blood and feces samples were collected on the first and last day of each period. After feeding with the addition of rice bran, BCS increased by 1.17 units on a 9-point scale. The experiment showed an increase in the total number of bacteria and methanogens inhabiting the cecum of horses. This can lead to better digestion of carbohydrates, absorption of nutrients, and, consequently, increased body weight. No differences occurred in the hematology and serum biochemistry indices of horses fed a diet including rice bran, except for the amount of serum globulin and the albumin to globulin ratio. Rice bran affected essential serum fatty acid profile (increased PUFA and decreased MUFA), which confirmed the possibility to use diet as a serum fatty acids profile modulator.
