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Acetic and citric acids effect the type II secretion system and decrease the metabolic activities of salmon spoilage-related Rahnella aquatilis KM05

cris.virtual.author-orcid0000-0002-3574-3117
cris.virtual.author-orcid0000-0002-3626-4388
cris.virtual.author-orcid0000-0002-8601-3536
cris.virtualsource.author-orcide0ec9333-c31e-44fd-9447-b159909e62c3
cris.virtualsource.author-orcid6eeeaac0-fbad-41dd-a73f-512626fd33cf
cris.virtualsource.author-orcidd5dfe19f-4d96-409d-9404-27a4f41fdb18
dc.abstract.enRahnella aquatilis causes seafoods to spoil by metabolizing sulfur-containing amino acids and/or proteins, producing H2S in products. The type II secretion system (T2SS) regulates the transport of proteases from the cytoplasm to the surrounding environment and promotes bacterial growth at low temperatures. To prevent premature fish spoilage, new solutions for inhibiting the T2SS of bacteria should be researched. In this study, global transcriptome sequencing was used to analyze the spoilage properties of R. aquatilis KM05. Two of the mapped genes/coding sequences (CDSs) were matched to the T2SS, namely, qspF and gspE, and four of the genes/CDSs, namely, ftsH, rseP, ptrA and pepN, were matched to metalloproteases or peptidases in R. aquatilis KM05. Subinhibitory concentrations of citric (18 µM) and acetic (41 µM) acids caused downregulation of T2SS-related genes (range from − 1.0 to -4.5) and genes involved in the proteolytic activities of bacteria (range from − 0.5 to -4.0). The proteolytic activities of R. aquatilis KM05 in vitro were reduced by an average of 40%. The in situ experiments showed the antimicrobial properties of citric and acetic acids against R. aquatilis KM05; the addition of an acidulant to salmon fillets limited microbial growth. Citric and acetic acids extend the shelf life of fish-based products and prevent food waste.
dc.affiliationWydział Nauk o Żywności i Żywieniu
dc.affiliationWydział Rolnictwa, Ogrodnictwa i Biotechnologii
dc.affiliation.instituteKatedra Biotechnologii i Mikrobiologii Żywności
dc.affiliation.instituteKatedra Biochemii i Biotechnologii
dc.contributor.authorMyszka, Kamila
dc.contributor.authorWolko, Łukasz
dc.contributor.authorBorkowska, Monika
dc.date.access2024-09-12
dc.date.accessioned2024-09-12T11:17:24Z
dc.date.available2024-09-12T11:17:24Z
dc.date.copyright2024-08-08
dc.date.issued2024
dc.description.abstract<jats:title>Abstract</jats:title><jats:p><jats:italic>Rahnella aquatilis</jats:italic> causes seafoods to spoil by metabolizing sulfur-containing amino acids and/or proteins, producing H<jats:sub>2</jats:sub>S in products. The type II secretion system (T2SS) regulates the transport of proteases from the cytoplasm to the surrounding environment and promotes bacterial growth at low temperatures. To prevent premature fish spoilage, new solutions for inhibiting the T2SS of bacteria should be researched. In this study, global transcriptome sequencing was used to analyze the spoilage properties of <jats:italic>R. aquatilis</jats:italic> KM05. Two of the mapped genes/coding sequences (CDSs) were matched to the T2SS, namely, <jats:italic>qspF</jats:italic> and <jats:italic>gspE</jats:italic>, and four of the genes/CDSs, namely, <jats:italic>ftsH</jats:italic>,<jats:italic> rseP</jats:italic>,<jats:italic> ptrA</jats:italic> and <jats:italic>pepN</jats:italic>, were matched to metalloproteases or peptidases in <jats:italic>R. aquatilis</jats:italic> KM05. Subinhibitory concentrations of citric (18 µM) and acetic (41 µM) acids caused downregulation of T2SS-related genes (range from − 1.0 to -4.5) and genes involved in the proteolytic activities of bacteria (range from − 0.5 to -4.0). The proteolytic activities of <jats:italic>R. aquatilis</jats:italic> KM05 in vitro were reduced by an average of 40%. The in situ experiments showed the antimicrobial properties of citric and acetic acids against <jats:italic>R. aquatilis</jats:italic> KM05; the addition of an acidulant to salmon fillets limited microbial growth. Citric and acetic acids extend the shelf life of fish-based products and prevent food waste.</jats:p>
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0.00
dc.description.if4
dc.description.points70
dc.description.versionfinal_published
dc.description.volume40
dc.identifier.doi10.1007/s11274-024-04101-z
dc.identifier.eissn1573-0972
dc.identifier.issn0959-3993
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/1740
dc.identifier.weblinkhttps://link.springer.com/article/10.1007/s11274-024-04101-z
dc.languageen
dc.relation.ispartofWorld Journal of Microbiology and Biotechnology
dc.relation.pagesart. 294
dc.rightsCC-BY
dc.sciencecloudsend
dc.subject.enSpoilage of seafoods
dc.subject.enRahnella aquatilis
dc.subject.enRNA-seq
dc.subject.enCitric acid
dc.subject.enAcetic acid
dc.subject.enT2SS
dc.titleAcetic and citric acids effect the type II secretion system and decrease the metabolic activities of salmon spoilage-related Rahnella aquatilis KM05
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue10
oaire.citation.volume40