Simple item page
 

Expression of adropin in the reproductive organs of healthy female dogs and those with cystic endometrial hyperplasia, pyometra, and ovarian cysts

cris.virtual.author-orcid0000-0002-4563-3798
cris.virtual.author-orcid0000-0001-9874-6895
cris.virtual.author-orcid0000-0002-3651-0972
cris.virtual.author-orcid0000-0002-6576-8972
cris.virtual.author-orcid0000-0001-5046-7416
cris.virtual.author-orcid0000-0002-1610-0589
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid0000-0002-7227-0654
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid0000-0003-4059-1362
cris.virtualsource.author-orcid2887b0c9-4f57-4235-a68f-057f9a3211f9
cris.virtualsource.author-orcid6fc589c0-a9dd-4835-bb57-7f31154633df
cris.virtualsource.author-orcid652e209d-434a-47b3-b5fb-64c0aac5a84c
cris.virtualsource.author-orcidf9afb1ae-f16d-4b00-9b01-bceecb2fb187
cris.virtualsource.author-orcid8cc943c4-fa09-421d-9e2b-84b33ec18db9
cris.virtualsource.author-orcidcfb82b96-7ef9-4b03-b4fb-1a3075fd805e
cris.virtualsource.author-orcid05a39e72-6677-4215-8bd3-b2564dec7817
cris.virtualsource.author-orcidad732626-b998-4c32-a9dc-82d9928533d8
cris.virtualsource.author-orcid18eda7dc-c10c-4267-bed0-38a3cc91af5c
cris.virtualsource.author-orcid9118c61a-b066-4efc-a7af-486f9ebf9021
dc.abstract.enAdropin, a peptide encoded by the energy homeostasis-associated gene (ENHO), is expressed in multiple tissues. It regulates glucose and lipid metabolism, as well as immune responses and exerts anti-inflammatory effects in various diseases. It was found that adropin may also modulate the female reproductive system. The objective of this study was to determine the expression of Enho and adropin production in the canine ovary (in healthy individuals and those with ovarian cysts) and in the endometrium of healthy female dogs and those affected by cystic endometrial hyperplasia (CEH) and pyometra. The analyses used in the study included RT-qPCR, Western blot, and immunofluorescent assays. Moreover, concentration of adropin was detected by ELISA assay in plasma samples. The results demonstrated higher Enho expression and adropin production in healthy animals compared to female dogs suffering from pyometra disease. The results also showed elevated adropin production and its blood concentrations in CEH-affected bitches. In ovarian tissues, higher peptide expression was shown in animals with healthy gonads and coexisting uterus disorders (such as CEH or pyometra). In cases of ovarian cysts, significantly lower expression of both Enho mRNA and adropin production compared to healthy ovarian tissues was detected. The presented studies expand our knowledge of adropin expression and localization in canine reproductive organs during physiological and pathological conditions.
dc.affiliationWydział Medycyny Weterynaryjnej i Nauk o Zwierzętach
dc.affiliation.instituteKatedra Nauk Przedklinicznych i Chorób Zakaźnych
dc.affiliation.instituteKatedra Chorób Wewnętrznych i Diagnostyki
dc.affiliation.instituteKatedra Fizjologii, Biochemii i Biostruktury Zwierząt
dc.affiliation.instituteKatedra Genetyki i Podstaw Hodowli Zwierząt​​
dc.contributor.authorRybska, Marta
dc.contributor.authorCzyżewska-Dors, Ewelina
dc.contributor.authorWojciechowicz, Tatiana
dc.contributor.authorSowińska, Natalia
dc.contributor.authorŁukomska, Anna
dc.contributor.authorAugustyniak, Agata
dc.contributor.authorSzyguła, Aleksandra
dc.contributor.authorGogulski, Maciej
dc.contributor.authorWąsowska, Barbara
dc.contributor.authorSkrzypski, Marek
dc.date.access2025-11-21
dc.date.accessioned2025-11-21T09:36:01Z
dc.date.available2025-11-21T09:36:01Z
dc.date.copyright2025-11-05
dc.date.issued2025
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if3,1
dc.description.numberDecember 2025
dc.description.points140
dc.description.versionfinal_published
dc.description.volume314
dc.identifier.doi10.1016/j.tvjl.2025.106478
dc.identifier.eissn1532-2971
dc.identifier.issn1090-0233
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/6060
dc.identifier.weblinkhttps://www.sciencedirect.com/science/article/pii/S1090023325001820?via%3Dihub
dc.languageen
dc.relation.ispartofVeterinary Journal
dc.relation.pagesart. 106478
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOTHER
dc.subject.encanine
dc.subject.enovary
dc.subject.enuterine
dc.subject.enCEH
dc.subject.enpyometra
dc.subject.enadropin
dc.titleExpression of adropin in the reproductive organs of healthy female dogs and those with cystic endometrial hyperplasia, pyometra, and ovarian cysts
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.volume314