Simple item page
 

Neuropeptide B promotes differentiation of rodent white preadipocytes into mature adipocytes

cris.lastimport.scopus2025-10-23T06:55:05Z
cris.virtual.author-orcid0000-0002-3651-0972
cris.virtual.author-orcid0000-0002-0412-5578
cris.virtual.author-orcid0000-0002-1948-6544
cris.virtual.author-orcid0000-0003-4059-1362
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid652e209d-434a-47b3-b5fb-64c0aac5a84c
cris.virtualsource.author-orcid1d7e7a9f-dfd0-4ed2-9512-a9aa9cf4ba27
cris.virtualsource.author-orcidb5ca3f7e-8c5b-47d7-b9c2-582f5ffa0e29
cris.virtualsource.author-orcid9118c61a-b066-4efc-a7af-486f9ebf9021
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.abstract.enNeuropeptide B (NPB) modulates energy homeostasis and metabolism through activation of NPBWR1 and NPBWR2 in humans and NPBWR1 in rodents. Recently, we reported that NPB promotes adipogenesis in rat brown preadipocytes. In the present study, we evaluated the effects of NPB on proliferation and differentiation into mature adipocytes of white rat preadipocytes and 3T3-L1 cells. We found the expression of NPBWR1 and NPB on mRNA and protein level in rat white preadipocytes and 3T3-L1 cells. NPB increased expression of mRNA and protein production of adipogenic genes (PPARγ, C/EBPβ, CEBPα and FABP4) in rat preadipocytes and 3T3-L1 cells during the differentiation process. Furthermore, NPB stimulated lipid accumulation in rat preadipocytes and 3T3-L1 cells. In addition, we found that NPB promotes phosphorylation of p38 kinase in rat preadipocytes and 3T3-L1 cells. NPB failed to stimulate expression of proadipogenic genes in the presence of p38 inhibitor. NPB failed to modulate viability and proliferation of rat preadipocytes and 3T3-L1 cells. Taken together, we report that NPB promotes differentiation of rodent preadipocytes via p38-dependent mechanism. NPB does not modulate viability and proliferation of rat preadipocytes and 3T3-L1 cells.
dc.affiliationWydział Medycyny Weterynaryjnej i Nauk o Zwierzętach
dc.affiliation.instituteKatedra Fizjologii, Biochemii i Biostruktury ZwierzÄ…t
dc.contributor.authorWojciechowicz, Tatiana
dc.contributor.authorSzczepankiewicz, Dawid
dc.contributor.authorStrowski, M.Z.
dc.contributor.authorNowak, Krzysztof W.
dc.contributor.authorSkrzypski, Marek
dc.date.accessioned2025-07-30T08:39:03Z
dc.date.available2025-07-30T08:39:03Z
dc.date.issued2023
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if3,8
dc.description.number15 February 2023
dc.description.points100
dc.description.volume562
dc.identifier.doi10.1016/j.mce.2023.111850
dc.identifier.eissn1872-8057
dc.identifier.issn0303-7207
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/4018
dc.languageen
dc.relation.ispartofMolecular and Cellular Endocrinology
dc.relation.pagesart. 111850
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enadipocytes
dc.subject.enadipogenesis
dc.subject.endifferentiation
dc.subject.enneuropeptide B
dc.subject.enpreadipocytes
dc.subject.enproliferation
dc.subject.en3T3-L1
dc.subject.enrat
dc.titleNeuropeptide B promotes differentiation of rodent white preadipocytes into mature adipocytes
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.volume562