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LC-QTOF-MS evaluation of rabbit-specific peptide markers for meat quantitation

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cris.virtual.author-orcid0000-0002-6331-5726
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cris.virtualsource.author-orcid6b8ff0a0-7556-4635-ae2c-064721f8c43a
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dc.abstract.enTen rabbit-specific tryptic peptide markers and one marker peptide specific to both rabbit and hare were evaluated for mass signal linearity in binary meat mixtures using liquid chromatography-quadrupole time-of-flight-mass spectrometry. Seven meat mixtures containing chicken and varying percentages of rabbit (1%, 5%, 10%, 30%, 60%, 90%, and 100%) were analyzed. Additionally, the signal linearity of twelve peptide markers for chicken meat was examined. The best candidate peptides for the quantification of meat content were selected. Five of eleven peptides for rabbit meat and five of twelve peptides for chicken meat showed good linearity (R2 > 0.97). The limits of detection and limits of quantification for these markers were in the range of 0.43–1.91% [w/w] and 1.44–6.38 % [w/w], respectively. The method allowed determination of the percentage content of rabbit and chicken meat in two- and three-component meat mixtures with good accuracy. The preliminary quantification data provide a starting point for developing label-free and absolute quantification methods for rabbit and chicken meat using multiple reaction monitoring of peptide markers.
dc.affiliationWydział Nauk o Żywności i Żywieniu
dc.affiliation.instituteKatedra Technologii Mięsa
dc.contributor.authorStachniuk, Anna
dc.contributor.authorKozub, Anna
dc.contributor.authorCzeczko, Renata
dc.contributor.authorMontowska, Magdalena
dc.contributor.authorFornal, Emilia
dc.date.access2026-02-27
dc.date.accessioned2026-03-09T08:40:37Z
dc.date.available2026-03-09T08:40:37Z
dc.date.copyright2022-06-15
dc.date.issued2022
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if3,6
dc.description.number2
dc.description.points140
dc.description.versionfinal_published
dc.description.volume30
dc.identifier.doi10.38212/2224-6614.3403
dc.identifier.issn1021-9498
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/7724
dc.identifier.weblinkhttp://www.jfda-online.com/journal/vol30/iss2/4/
dc.languageen
dc.relation.ispartofJournal of Food and Drug Analysis
dc.relation.pages223-233
dc.rightsCC-BY-NC-ND
dc.sciencecloudnosend
dc.share.typeOPEN_JOURNAL
dc.subject.enpeptide markers
dc.subject.enlinearity
dc.subject.enrabbit
dc.subject.enmeat products
dc.subject.enliquid chromatography-mass spectrometry
dc.titleLC-QTOF-MS evaluation of rabbit-specific peptide markers for meat quantitation
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue2
oaire.citation.volume30