Szanowni Państwo, w związku z bardzo dużą ilością zgłoszeń, rejestracją danych w dwóch systemach bibliograficznych, a jednocześnie zmniejszonym zespołem redakcyjnym proces rejestracji i redakcji opisów publikacji jest wydłużony. Bardzo przepraszamy za wszelkie niedogodności i dziękujemy za Państwa wyrozumiałość.
Repository logoRepository logoRepository logoRepository logo
Repository logoRepository logoRepository logoRepository logo
  • Communities & Collections
  • Research Outputs
  • Employees
  • AAAHigh contrastHigh contrast
    EN PL
    • Log In
      Have you forgotten your password?
AAAHigh contrastHigh contrast
EN PL
  • Log In
    Have you forgotten your password?
  1. Home
  2. Bibliografia UPP
  3. Bibliografia UPP
  4. The Influence of Lead and Acyrthosiphon pisum (Harris) on Generation of Pisum sativum Defense Signaling Molecules and Expression of Genes Involved in Their Biosynthesis
 
Full item page
Options

The Influence of Lead and Acyrthosiphon pisum (Harris) on Generation of Pisum sativum Defense Signaling Molecules and Expression of Genes Involved in Their Biosynthesis

Type
Journal article
Language
English
Date issued
2023
Author
Woźniak, Agnieszka
Kęsy, Jacek
Glazińska, Paulina
Glinkowski, Wojciech
Narożna, Dorota 
Bocianowski, Jan 
Rucińska-Sobkowiak, Renata
Mai, Van Chung
Krzesiński, Włodzimierz 
Samardakiewicz, Sławomir
Borowiak-Sobkowiak, Beata 
Labudda, Mateusz
Jeandet, Philippe
Morkunas, Iwona 
Faculty
Wydział Rolnictwa, Ogrodnictwa i Biotechnologii
PBN discipline
agriculture and horticulture
Journal
International Journal of Molecular Sciences
ISSN
1661-6596
DOI
10.3390/ijms241310671
Web address
https://www.mdpi.com/1422-0067/24/13/10671
Volume
24
Number
13
Pages from-to
art. 10671
Abstract (EN)
The main aim of this study was to understand the regulation of the biosynthesis of phytohormones as signaling molecules in the defense mechanisms of pea seedlings during the application of abiotic and biotic stress factors. It was important to identify this regulation at the molecular level in Pisum sativum L. seedlings under the influence of various concentrations of lead—i.e., a low concentration increasing plant metabolism, causing a hormetic effect, and a high dose causing a sublethal effect—and during feeding of a phytophagous insect with a piercing-sucking mouthpart—i.e., pea aphid (Acyrthosiphon pisum (Harris)). The aim of the study was to determine the expression level of genes encoding enzymes of the biosynthesis of signaling molecules such as phytohormones—i.e., jasmonates (JA/MeJA), ethylene (ET) and abscisic acid (ABA). Real-time qPCR was applied to analyze the expression of genes encoding enzymes involved in the regulation of the biosynthesis of JA/MeJA (lipoxygenase 1 (LOX1), lipoxygenase 2 (LOX2), 12-oxophytodienoate reductase 1 (OPR1) and jasmonic acid-amido synthetase (JAR1)), ET (1-aminocyclopropane-1-carboxylate synthase 3 (ACS3)) and ABA (9-cis-epoxycarotenoid dioxygenase (NCED) and aldehyde oxidase 1 (AO1)). In response to the abovementioned stress factors—i.e., abiotic and biotic stressors acting independently or simultaneously—the expression of the LOX1, LOX2, OPR1, JAR1, ACS3, NCED and AO1 genes at both sublethal and hormetic doses increased. Particularly high levels of the relative expression of the tested genes in pea seedlings growing at sublethal doses of lead and colonized by A. pisum compared to the control were noticeable. A hormetic dose of lead induced high expression levels of the JAR1, OPR1 and ACS3 genes, especially in leaves. Moreover, an increase in the concentration of phytohormones such as jasmonates (JA and MeJA) and aminococyclopropane-1-carboxylic acid (ACC)-ethylene (ET) precursor was observed. The results of this study indicate that the response of pea seedlings to lead and A. pisum aphid infestation differed greatly at both the gene expression and metabolic levels. The intensity of these defense responses depended on the organ, the metal dose and direct contact of the stress factor with the organ.
Keywords (EN)
  • lead

  • pea aphid

  • jasmonates

  • ethylene

  • genes encoding enzymes of phytoh...

  • Pisum sativum

License
cc-bycc-by CC-BY - Attribution
Open access date
June 26, 2023
Fundusze Europejskie
  • About repository
  • Contact
  • Privacy policy
  • Cookies

Copyright 2025 Uniwersytet Przyrodniczy w Poznaniu

DSpace Software provided by PCG Academia