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Study of Antibiotic Resistance Potential of Spiders Bacteriota and Comparison With the Antibacterial Effect of Essential Oils

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dc.abstract.enInsect microorganisms significantly affect the diet, well-being and behavior of their hosts. However, the microbiota of spiders, which are important natural enemies of pests, is still largely unknown. To gain insight into the bacterial composition of spiders and their possible roles, we collected five different spider species: Pardosa hortensis, Pholcus phalangioides, Steatoda bipunctata, Steatoda triangulosa (Walckenaer, 1802), and Tegenaria domestica (Clerck, 1757). Mass spectrometry was used to identify the microbiota of each species. After identifying the microbes, the resistence to antibiotics was measured and compared their antibacterial activity with essential oils derived from plants. The antibiotics tested for species-specific antibacterial activity were imipenem (IPM, 30 mg), ciprofloxacin (CIP, 30 µg/disc), linezolid (LZD, 30 µg/disc), tobramycin (TOB, 30 µg/disc), tigecycline (TGC, 30 µg/disc), and tetracycline (TE, 30 µg/disc). We also tested the antibacterial activity of essential oils from Cedar atlantica, Illicium verum, and Pelargonium graveolens. The most frequently identified species were Bacillus mycoides from P. phalangioides, Stutzerimonas chloritidismutans from S. bipunctata, Aerococcus viridans and other species from S. triangulosa, and Bacillus mycoides and Enterococcus faecium from T. domestica. Antibiotic resistance was present in one-third of the isolates, with most antibiotic-resistant bacteria found in T. domestica. The essential oils exhibited high antibacterial activity, particularly against Staphylococcus epidermidis, Bacillus pumilus, Priestia megaterium, and Moraxella osloensis.
dc.affiliationWydział Nauk o Żywności i Żywieniu
dc.affiliation.instituteKatedra Technologii Żywności Pochodzenia Roślinnego
dc.contributor.authorKačániová, Miroslava
dc.contributor.authorElizondo‐Luevano, Joel Horacio
dc.contributor.authorBan, Zhaojun
dc.contributor.authorBabošová, Mária
dc.contributor.authorPorhajašová, Jana Ivanič
dc.contributor.authorKollár, Ján
dc.contributor.authorKowalczewski, Przemysław Łukasz
dc.contributor.authorGarzoli, Stefania
dc.date.access2025-11-21
dc.date.accessioned2025-11-21T11:33:01Z
dc.date.available2025-11-21T11:33:01Z
dc.date.copyright2025-11-07
dc.date.issued2025
dc.description.abstract<jats:title>ABSTRACT</jats:title> <jats:p> Insect microorganisms significantly affect the diet, well‐being and behavior of their hosts. However, the microbiota of spiders, which are important natural enemies of pests, is still largely unknown. To gain insight into the bacterial composition of spiders and their possible roles, we collected five different spider species: <jats:italic>Pardosa hortensis</jats:italic> , <jats:italic>Pholcus phalangioides</jats:italic> , <jats:italic>Steatoda bipunctata</jats:italic> , <jats:italic>Steatoda triangulosa</jats:italic> (Walckenaer, 1802), and <jats:italic>Tegenaria domestica</jats:italic> (Clerck, 1757). Mass spectrometry was used to identify the microbiota of each species. After identifying the microbes, the resistence to antibiotics was measured and compared their antibacterial activity with essential oils derived from plants. The antibiotics tested for species‐specific antibacterial activity were imipenem (IPM, 30 mg), ciprofloxacin (CIP, 30 µg/disc), linezolid (LZD, 30 µg/disc), tobramycin (TOB, 30 µg/disc), tigecycline (TGC, 30 µg/disc), and tetracycline (TE, 30 µg/disc). We also tested the antibacterial activity of essential oils from <jats:italic>Cedar atlantica</jats:italic> , <jats:italic>Illicium verum</jats:italic> , and <jats:italic>Pelargonium graveolens</jats:italic> . The most frequently identified species were <jats:italic>Bacillus mycoides</jats:italic> from <jats:italic>P. phalangioides</jats:italic> , <jats:italic>Stutzerimonas chloritidismutans</jats:italic> from <jats:italic>S. bipunctata</jats:italic> , <jats:italic>Aerococcus viridans</jats:italic> and other species from <jats:italic>S. triangulosa</jats:italic> , and <jats:italic>Bacillus mycoides</jats:italic> and <jats:italic>Enterococcus faecium</jats:italic> from <jats:italic>T. domestica</jats:italic> . Antibiotic resistance was present in one‐third of the isolates, with most antibiotic‐resistant bacteria found in <jats:italic>T. domestica</jats:italic> . The essential oils exhibited high antibacterial activity, particularly against <jats:italic>Staphylococcus epidermidis</jats:italic> , <jats:italic>Bacillus pumilus</jats:italic> , <jats:italic>Priestia megaterium</jats:italic> , and <jats:italic>Moraxella osloensis</jats:italic> . </jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if4,6
dc.description.number6
dc.description.points70
dc.description.versionfinal_published
dc.description.volume14
dc.identifier.doi10.1002/mbo3.70145
dc.identifier.issn2045-8827
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/6063
dc.identifier.weblinkhttps://onlinelibrary.wiley.com/doi/10.1002/mbo3.70145
dc.languageen
dc.relation.ispartofMicrobiologyOpen
dc.relation.pagese70145
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOPEN_JOURNAL
dc.subject.enantibacterial resistance
dc.subject.enmass spectrometry
dc.subject.enspider microbiota
dc.subject.enzoonotic transmission
dc.titleStudy of Antibiotic Resistance Potential of Spiders Bacteriota and Comparison With the Antibacterial Effect of Essential Oils
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue6
oaire.citation.volume14