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Expression Profiling and Interaction Effects of Three R-Genes Conferring Resistance to Blackleg Disease in Brassica napus

cris.virtual.author-orcid0000-0002-8011-9487
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cris.virtualsource.author-orcid20597688-8be2-4b58-9e15-29f5ff8c53aa
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cris.virtualsource.author-orcid51a5a68b-106b-4e9d-bd9b-79d15d3ec0c1
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dc.abstract.enBrassica napus L. is one of the world’s most important oilseed crops. Blackleg disease is a serious, yield-limiting factor in the cultivation of oilseed rape. Genetic resistance is primarily conferred by major resistance (R) genes. In this study, we analyzed the expression of the blackleg resistance genes Rlm3, Rlm4, and Rlm7 following inoculation with the Leptosphaeria maculans isolate using the RT-qPCR method. Additionally, we demonstrated and assessed their interactions. The results showed that, while Rlm3 was weakly induced, Rlm4 and Rlm7 displayed variable expression post-inoculation. The correlation between phenotypic and genotypic similarity was low. This suggests that transcriptional responses do not fully explain resistance patterns. Furthermore, significant main effects of the analyzed genes, as well as two- and three-way interactions, were indicated. These results support current knowledge of gene-mediated resistance to blackleg in oilseed rape.
dc.abstract.languageen
dc.affiliationWydział Rolnictwa, Ogrodnictwa i Biotechnologii
dc.affiliation.instituteKatedra Genetyki i Hodowli Roślin
dc.affiliation.instituteKatedra Metod Matematycznych i Statystycznych
dc.contributor.authorNiemann, Janetta
dc.contributor.authorStarosta, Ewa
dc.contributor.authorKaczmarek, Joanna
dc.contributor.authorPawłowicz, Izabela
dc.contributor.authorBocianowski, Jan
dc.contributor.institutionUniwersytet Przyrodniczy w Poznaniu
dc.date.access2025-10-30
dc.date.accessioned2025-11-03T07:34:51Z
dc.date.available2025-11-03T07:34:51Z
dc.date.copyright2025-10-30
dc.date.issued2025
dc.description.abstract<jats:p>Brassica napus L. is one of the world’s most important oilseed crops. Blackleg disease is a serious, yield-limiting factor in the cultivation of oilseed rape. Genetic resistance is primarily conferred by major resistance (R) genes. In this study, we analyzed the expression of the blackleg resistance genes Rlm3, Rlm4, and Rlm7 following inoculation with the Leptosphaeria maculans isolate using the RT-qPCR method. Additionally, we demonstrated and assessed their interactions. The results showed that, while Rlm3 was weakly induced, Rlm4 and Rlm7 displayed variable expression post-inoculation. The correlation between phenotypic and genotypic similarity was low. This suggests that transcriptional responses do not fully explain resistance patterns. Furthermore, significant main effects of the analyzed genes, as well as two- and three-way interactions, were indicated. These results support current knowledge of gene-mediated resistance to blackleg in oilseed rape.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_research
dc.description.financecost11000,00
dc.description.if2,5
dc.description.number21
dc.description.points100
dc.description.versionfinal_published
dc.description.volume15
dc.identifier.doi10.3390/app152111613
dc.identifier.eissn2076-3417
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/5682
dc.identifier.weblinkhttps://www.mdpi.com/2076-3417/15/21/11613#:~:text=In%20this%20study%2C%20we%20analyzed%20the%20expression%20of,method.%20Additionally%2C%20we%20demonstrated%20and%20assessed%20their%20interactions.
dc.languageen
dc.pbn.affiliationagriculture and horticulture
dc.relation.ispartofApplied Sciences (Switzerland)
dc.relation.pagesart. 11613
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOPEN_JOURNAL
dc.subject.enBrassica napus
dc.subject.enL. maculans
dc.subject.enblackleg resistance genes
dc.subject.engene expression
dc.subject.engene interactions
dc.subtypeScientificCommunication
dc.titleExpression Profiling and Interaction Effects of Three R-Genes Conferring Resistance to Blackleg Disease in Brassica napus
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue21
oaire.citation.volume15