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Transcriptomic Characterization of Candidate Genes for Fusarium Resistance in Maize (Zea mays L.)

cris.virtual.author-orcid0000-0001-9516-8911
cris.virtual.author-orcid0000-0003-4445-5624
cris.virtual.author-orcid0000-0001-9250-4393
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cris.virtual.author-orcid0000-0002-0102-0084
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cris.virtualsource.author-orcid2faa4bbb-a129-4bec-8137-bd8eeedf40e0
cris.virtualsource.author-orcid36fc7bfc-89c8-4648-af52-05dd31161d2f
cris.virtualsource.author-orcidb449fb52-7ef2-4cac-9b3b-ec94daac3768
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cris.virtualsource.author-orcid51a5a68b-106b-4e9d-bd9b-79d15d3ec0c1
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dc.abstract.enFusarium diseases are among the most dangerous fungal diseases of plants. To date, there are no plant protectants that completely prevent fusariosis. Current breeding trends are therefore focused on increasing genetic resistance. While global modern maize breeding relies on various molecular genetics techniques, they are useless without a precise characterization of genomic regions that determine plant physiological responses to fungi. The aim of this study was thus to characterize the expression of candidate genes that were previously reported by our team as harboring markers linked to fusarium resistance in maize. The plant material included one susceptible and four resistant varieties. Biotic stress was induced in adult plants by inoculation with fungal spores under controlled conditions. qRT-PCR was performed. The analysis focused on four genes that encode for GDSL esterase/lipase (LOC100273960), putrescine hydroxycinnamyltransferase (LOC103649226), peroxidase 72 (LOC100282124), and uncharacterized protein (LOC100501166). Their expression showed differences between analyzed time points and varieties, peaking at 6 hpi. The resistant varieties consistently showed higher levels of expression compared to the susceptible variety, indicating their stronger defense responses. Moreover, to better understand the function of these genes, their expression in various organs and tissues was also evaluated using publicly available transcriptomic data. Our results are consistent with literature reports that clearly indicate the involvement of these genes in the resistance response to fusarium. Thus, they further emphasize the high usefulness of the previously selected markers in breeding programs to select fusarium-resistant maize genotypes.
dc.affiliationWydział Rolnictwa, Ogrodnictwa i Biotechnologii
dc.affiliation.instituteKatedra Genetyki i Hodowli Roślin
dc.affiliation.instituteKatedra Fitopatologii i Nasiennictwa
dc.affiliation.instituteKatedra Metod Matematycznych i Statystycznych
dc.contributor.authorSobiech, Aleksandra
dc.contributor.authorTomkowiak, Agnieszka
dc.contributor.authorJamruszka, Tomasz
dc.contributor.authorKosiada, Tomasz
dc.contributor.authorSpychała, Julia
dc.contributor.authorLenort, Maciej
dc.contributor.authorBocianowski, Jan
dc.date.access2025-08-20
dc.date.accessioned2025-08-20T06:30:51Z
dc.date.available2025-08-20T06:30:51Z
dc.date.copyright2025-08-06
dc.date.issued2025
dc.description.abstract<jats:p>Fusarium diseases are among the most dangerous fungal diseases of plants. To date, there are no plant protectants that completely prevent fusariosis. Current breeding trends are therefore focused on increasing genetic resistance. While global modern maize breeding relies on various molecular genetics techniques, they are useless without a precise characterization of genomic regions that determine plant physiological responses to fungi. The aim of this study was thus to characterize the expression of candidate genes that were previously reported by our team as harboring markers linked to fusarium resistance in maize. The plant material included one susceptible and four resistant varieties. Biotic stress was induced in adult plants by inoculation with fungal spores under controlled conditions. qRT-PCR was performed. The analysis focused on four genes that encode for GDSL esterase/lipase (LOC100273960), putrescine hydroxycinnamyltransferase (LOC103649226), peroxidase 72 (LOC100282124), and uncharacterized protein (LOC100501166). Their expression showed differences between analyzed time points and varieties, peaking at 6 hpi. The resistant varieties consistently showed higher levels of expression compared to the susceptible variety, indicating their stronger defense responses. Moreover, to better understand the function of these genes, their expression in various organs and tissues was also evaluated using publicly available transcriptomic data. Our results are consistent with literature reports that clearly indicate the involvement of these genes in the resistance response to fusarium. Thus, they further emphasize the high usefulness of the previously selected markers in breeding programs to select fusarium-resistant maize genotypes.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if3,3
dc.description.number8
dc.description.points100
dc.description.versionfinal_published
dc.description.volume14
dc.identifier.doi10.3390/pathogens14080779
dc.identifier.issn2076-0817
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/4298
dc.identifier.weblinkhttps://www.mdpi.com/2076-0817/14/8/779
dc.languageen
dc.relation.ispartofPathogens
dc.relation.pagesart. 779
dc.rightsCC-BY
dc.sciencecloudsend
dc.share.typeOPEN_JOURNAL
dc.subject.encandidate genes
dc.subject.enfusarium
dc.subject.enplant resistance
dc.titleTranscriptomic Characterization of Candidate Genes for Fusarium Resistance in Maize (Zea mays L.)
dc.title.volumeSpecial Issue Current Research on Fusarium: 2nd Edition
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue8
oaire.citation.volume14