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Influence of a multi-strain probiotic and zinc-glycine chelate, administered in ovo, on immune response in newly hatched chicks

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cris.virtual.author-orcid0000-0003-1772-7997
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cris.virtualsource.author-orcid95e7bfbc-b48c-4cbc-a67b-0c82494d303d
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dc.abstract.enIntroduction: The supplementation of chicken embryos with bioactive compounds may elicit a beneficial effect on the development of their gut microbiome and enhance protection against infectious agents after hatching. Therefore, this study aimed to evaluate the effect of in ovo co-supplementation with a multi-strain probiotic and zinc-glycine chelate on the levels of pro- and anti-inflammatory cytokines, acute-phase proteins, and immunoglobulins in the peripheral blood and tissues of broiler chickens on the day of hatching and 7 days post hatching. The effect of supplementation on the growth parameters of chickens was assessed as well. Methods: The study was conducted on 1,500 hatching eggs from a broiler breeding flock (Ross × Ross 308) at 36 weeks. ELISA kits were used to determine levels of acute-phase proteins and immunoglobulins. Expression of immunoglobulins was determined by means of qRT-PCR. Results: The results indicate enhanced synthesis of acute-phase proteins in the liver and increased levels of serum amyloid A in the small intestine tissue, as well as IgA and IgM mRNA and suppressed synthesis of pro-inflammatory cytokines IFN-γ and TNF-α. During the cumulative experimental period (days 0–42), the mean body weight gain (BWG) and feed intake (FI) in the group supplemented with a multi-strain probiotic were statistically significantly lower than the control group. Discussion: It may be concluded that the combined in ovo use of a multi-strain probiotic and Zn-Gly chelate modulates the immune response, helps maintain the balance between the synthesis of Th1 and Th2 cytokines, inhibits inflammatory processes, and stimulates immune system development.
dc.affiliationWydział Medycyny Weterynaryjnej i Nauk o Zwierzętach
dc.affiliation.instituteKatedra Hodowli Zwierząt i Oceny Surowców
dc.contributor.authorCiszewski, Artur
dc.contributor.authorJarosz, Łukasz S.
dc.contributor.authorGrądzki, Zbigniew
dc.contributor.authorMarek, Agnieszka
dc.contributor.authorKaczmarek, Beata
dc.contributor.authorHejdysz, Marcin
dc.contributor.authorRysiak, Anna
dc.date.access2025-11-14
dc.date.accessioned2025-11-14T11:55:20Z
dc.date.available2025-11-14T11:55:20Z
dc.date.copyright2025-09-22
dc.date.issued2025
dc.description.abstract<jats:sec><jats:title>Introduction</jats:title><jats:p>The supplementation of chicken embryos with bioactive compounds may elicit a beneficial effect on the development of their gut microbiome and enhance protection against infectious agents after hatching. Therefore, this study aimed to evaluate the effect of <jats:italic>in ovo</jats:italic> co-supplementation with a multi-strain probiotic and zinc-glycine chelate on the levels of pro- and anti-inflammatory cytokines, acute-phase proteins, and immunoglobulins in the peripheral blood and tissues of broiler chickens on the day of hatching and 7 days post hatching. The effect of supplementation on the growth parameters of chickens was assessed as well.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>The study was conducted on 1,500 hatching eggs from a broiler breeding flock (Ross × Ross 308) at 36 weeks. ELISA kits were used to determine levels of acute-phase proteins and immunoglobulins. Expression of immunoglobulins was determined by means of qRT-PCR.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>The results indicate enhanced synthesis of acute-phase proteins in the liver and increased levels of serum amyloid A in the small intestine tissue, as well as IgA and IgM mRNA and suppressed synthesis of pro-inflammatory cytokines IFN-γ and TNF-α. During the cumulative experimental period (days 0–42), the mean body weight gain (BWG) and feed intake (FI) in the group supplemented with a multi-strain probiotic were statistically significantly lower than the control group.</jats:p></jats:sec><jats:sec><jats:title>Discussion</jats:title><jats:p>It may be concluded that the combined in ovo use of a multi-strain probiotic and Zn-Gly chelate modulates the immune response, helps maintain the balance between the synthesis of Th1 and Th2 cytokines, inhibits inflammatory processes, and stimulates immune system development.</jats:p></jats:sec>
dc.description.accesstimeat_publication
dc.description.bibliographybibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if3,4
dc.description.points100
dc.description.versionfinal_published
dc.description.volume16
dc.identifier.doi10.3389/fphys.2025.1646143
dc.identifier.issn1664-042X
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/5900
dc.identifier.weblinkhttps://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2025.1646143/full
dc.languageen
dc.relation.ispartofFrontiers in Physiology
dc.relation.pagesart. 1646143
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOPEN_JOURNAL
dc.subject.enin ovo
dc.subject.enchelated zinc
dc.subject.enmulti-strain probiotic
dc.subject.encytokines
dc.subject.enacute-phase proteins
dc.subject.enimmunoglobulins
dc.titleInfluence of a multi-strain probiotic and zinc-glycine chelate, administered in ovo, on immune response in newly hatched chicks
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.volume16