The Impact of Process Parameters on 1,3-Propanediol Production and 3-Hydroxypropionaldehyde Accumulation in Fed-Batch Fermentation of Glycerol with Citrobacter freundii AD119
| cris.virtual.author-orcid | 0000-0002-4580-354X | |
| cris.virtual.author-orcid | 0000-0001-8851-912X | |
| cris.virtual.author-orcid | 0000-0002-6720-891X | |
| cris.virtual.author-orcid | 0000-0002-0698-3808 | |
| cris.virtualsource.author-orcid | 897f1035-fcd0-4882-9b40-77273ff2fd96 | |
| cris.virtualsource.author-orcid | fa773a3b-d0b2-4feb-b51f-0901bb0ce777 | |
| cris.virtualsource.author-orcid | 7c4fb780-333e-446e-957f-1ab650ac136d | |
| cris.virtualsource.author-orcid | 40b1ae89-2197-4059-8c15-4c792dc04582 | |
| dc.abstract.en | Microbial production of 1,3-propanediol (1,3-PD) has attracted the interest of scientists for decades. Its product offers an environmentally friendly and sustainable alternative to fossil-based raw materials for chemical synthesis. Citrobacter freundii is one of the natural producers of 1,3-PD known for its ability to yield it in significant titers. An efficient bioprocess requires an in-depth understanding of the factors that influence the performance of its biocatalyst. The effects of pH, temperature, stirring rate, and substrate concentration on glycerol fermentation in fed-batch cultures of C. freundii AD119 were investigated in this study. In addition to monitoring the kinetics of substrate utilization and the formation of the final products, the concentration of 3-hydroxypropionaldehyde (3-HPA), an inhibitory intermediate of glycerol bioconversion, was analyzed. When the optimal working conditions were used (pH 7.0, temperature 30 °C, stirring rate of 80 rpm, and glycerol concentration below 15 g/L during the fed-batch phase), 53.44 g/L of 1,3-PD were obtained. When the process was performed at temperatures of 33 °C or higher or in acidic pH (6.5), an elevated concentration of 3-HPA was observed and the process halted prematurely. | |
| dc.affiliation | Wydział Nauk o Żywności i Żywieniu | |
| dc.affiliation.institute | Katedra Biotechnologii i Mikrobiologii Żywności | |
| dc.affiliation.institute | Katedra Mleczarstwa i Inżynierii Procesowej | |
| dc.contributor.author | Drożdżyńska, Agnieszka | |
| dc.contributor.author | Kubiak, Piotr | |
| dc.contributor.author | Wawrzyniak, Jolanta | |
| dc.contributor.author | Czaczyk, Katarzyna | |
| dc.date.access | 2025-08-21 | |
| dc.date.accessioned | 2025-08-21T10:23:28Z | |
| dc.date.available | 2025-08-21T10:23:28Z | |
| dc.date.copyright | 2023-06-10 | |
| dc.date.issued | 2023 | |
| dc.description.abstract | <jats:p>Microbial production of 1,3-propanediol (1,3-PD) has attracted the interest of scientists for decades. Its product offers an environmentally friendly and sustainable alternative to fossil-based raw materials for chemical synthesis. Citrobacter freundii is one of the natural producers of 1,3-PD known for its ability to yield it in significant titers. An efficient bioprocess requires an in-depth understanding of the factors that influence the performance of its biocatalyst. The effects of pH, temperature, stirring rate, and substrate concentration on glycerol fermentation in fed-batch cultures of C. freundii AD119 were investigated in this study. In addition to monitoring the kinetics of substrate utilization and the formation of the final products, the concentration of 3-hydroxypropionaldehyde (3-HPA), an inhibitory intermediate of glycerol bioconversion, was analyzed. When the optimal working conditions were used (pH 7.0, temperature 30 °C, stirring rate of 80 rpm, and glycerol concentration below 15 g/L during the fed-batch phase), 53.44 g/L of 1,3-PD were obtained. When the process was performed at temperatures of 33 °C or higher or in acidic pH (6.5), an elevated concentration of 3-HPA was observed and the process halted prematurely.</jats:p> | |
| dc.description.accesstime | at_publication | |
| dc.description.bibliography | il., bibliogr. | |
| dc.description.finance | publication_nocost | |
| dc.description.financecost | 0,00 | |
| dc.description.if | 2,5 | |
| dc.description.number | 12 | |
| dc.description.points | 100 | |
| dc.description.version | final_published | |
| dc.description.volume | 13 | |
| dc.identifier.doi | 10.3390/app13127003 | |
| dc.identifier.issn | 2076-3417 | |
| dc.identifier.uri | https://sciencerep.up.poznan.pl/handle/item/4327 | |
| dc.identifier.weblink | https://www.mdpi.com/2076-3417/13/12/7003 | |
| dc.language | en | |
| dc.relation.ispartof | Applied Sciences (Switzerland) | |
| dc.relation.pages | art. 7003 | |
| dc.rights | CC-BY | |
| dc.sciencecloud | nosend | |
| dc.share.type | OPEN_JOURNAL | |
| dc.subject.en | 1.3-PD | |
| dc.subject.en | pH-stat fed-batch fermentation | |
| dc.subject.en | optimization of culture conditions | |
| dc.subject.en | crude/waste glycerol | |
| dc.subject.en | reuterin | |
| dc.subject.en | glycerol feeding strategies | |
| dc.title | The Impact of Process Parameters on 1,3-Propanediol Production and 3-Hydroxypropionaldehyde Accumulation in Fed-Batch Fermentation of Glycerol with Citrobacter freundii AD119 | |
| dc.type | JournalArticle | |
| dspace.entity.type | Publication | |
| oaire.citation.issue | 12 | |
| oaire.citation.volume | 13 |