The Promising Prospective Technology for Biofuel Production with Mixed Cultures Using Sorghum Grain (Sorghum bicolor (L.) Moench) Grown in a Temperate Climate: Characteristics of Fermentation Factors and Volatile Compounds Analysis

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cris.virtual.author-orcid0000-0002-6568-8155
cris.virtual.author-orcid0000-0002-4176-6358
cris.virtual.author-orcid0000-0003-4570-7221
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cris.virtualsource.author-orcidcb3305a8-f370-4ea4-b74d-97bd22a2d068
cris.virtualsource.author-orcid1ddb8111-48d2-4a19-a7fb-97562fc1691c
cris.virtualsource.author-orcid0bfd6d64-735f-474a-91ed-e325fff77417
cris.virtualsource.author-orciddafe00c4-99bb-45f5-8886-d6f261ff6cb3
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.abstract.enSorghum grain (Sorghum bicolor (L.) Moench) grown in a temperate climate was used as a feedstock for bioethanol production. Mixed cultures of Zymomonas mobilis and Saccharomyces cerevisiae were used for fermentation under the conditions and parameters established through the design of experiments (DOE, 2(K–p) standard design). The most effective parameters for the simultaneous saccharification and fermentation of the liquefied and granular starch fermentation methods were selected. Analyses revealed that the granular starch fermentation method (with Stargen 002) combined with mixed cultures yielded higher bioethanol by about an average of 5% compared to single cultures. Distillates obtained in fermentations with mixed cultures were investigated qualitatively and quantitatively for particular volatile byproducts. Distillates from fermentation with mixed cultures contained higher amounts of volatile byproducts (9.04–11.38 g/L) than single bacterial cultures (4.10–6.68 g/L) but less than single yeast cultures (9.27–13.14 g/L). These findings indicate the potential of mixed cultures in granular starch fermentation, at the same time suggesting that challenges such as selecting microbial consortia, accelerating the process, and ensuring consistent yields must be solved for widespread industrial application.
dc.affiliationWydział Nauk o Żywności i Żywieniu
dc.affiliation.instituteKatedra Technologii Żywności Pochodzenia Roślinnego
dc.contributor.authorSzambelan, Katarzyna Teresa
dc.contributor.authorJeleń, Henryk
dc.contributor.authorNowak, Jacek
dc.contributor.authorFrankowski, Jakub
dc.contributor.authorSzwengiel, Artur
dc.date.accessioned2026-01-07T06:51:19Z
dc.date.available2026-01-07T06:51:19Z
dc.date.issued2025
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if6,2
dc.description.number23
dc.description.points140
dc.description.volume73
dc.identifier.doi10.1021/acs.jafc.5c02582
dc.identifier.eissn1520-5118
dc.identifier.issn0021-8561
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/6600
dc.languageen
dc.relation.ispartofJournal of Agricultural and Food Chemistry
dc.relation.pages14408-14419
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.encofermentation
dc.subject.ensorghum grain
dc.subject.engranular starch
dc.subject.enbioethanol production
dc.subject.envolatile compounds
dc.titleThe Promising Prospective Technology for Biofuel Production with Mixed Cultures Using Sorghum Grain (Sorghum bicolor (L.) Moench) Grown in a Temperate Climate: Characteristics of Fermentation Factors and Volatile Compounds Analysis
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue23
oaire.citation.volume73