Simple item page
 

New Prospects for Improving Microspore Embryogenesis Induction in Highly Recalcitrant Winter Wheat Lines

cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid0000-0002-1041-4341
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid0000-0003-2480-855X
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid94714645-6b07-4d4d-bd16-1baacaa7ba63
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid07a12041-d6ed-46a8-8dc3-df09345a7119
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.abstract.enAmong various methods stimulating biological progress, double haploid (DH) technology, which utilizes the process of microspore embryogenesis (ME), is potentially the most effective. However, the process depends on complex interactions between many genetic, physiological and environmental variables, and in many cases, e.g., winter wheat, does not operate with the efficiency required for commercial use. Stress associated with low-temperature treatment, isolation and transfer to in vitro culture has been shown to disturb redox homeostasis and generate relatively high levels of reactive oxygen species (ROS), affecting microspore vitality. The aim of this study was to investigate whether controlled plant growth, specific tiller pre-treatment and culture conditions could improve the potential of microspores to cope with stress and effectively induce ME. To understand the mechanism of the stress response, hydrogen peroxide levels, total activity and the content of the most important low-molecular-weight antioxidants (glutathione and ascorbate), as well as the content of selected macro- (Mg, Ca, NA, K) and micronutrients (Mn, Zn, Fe, Cu, Mo) were determined. These analyses, combined with the cytological characteristics of the microspore suspensions, allowed us to demonstrate that an increased microspore vitality and stronger response to ME induction were associated with higher stress resistance based on more efficient ROS scavenging and nutrient management. It was shown that a modified procedure, combining a low temperature with mannitol and sodium selenate tiller pre-treatment, reduced oxidative stress and improved the effectiveness of ME in winter wheat lines.
dc.affiliationWydział Rolnictwa, Ogrodnictwa i Biotechnologii
dc.affiliation.instituteKatedra Genetyki i Hodowli Roślin
dc.contributor.authorDubas, Ewa
dc.contributor.authorKrzewska, Monika
dc.contributor.authorSurówka, Ewa
dc.contributor.authorKopeć, Przemysław
dc.contributor.authorSpringer, Agnieszka
dc.contributor.authorJanowiak, Franciszek
dc.contributor.authorWeigt, Dorota
dc.contributor.authorMikołajczyk, Sylwia
dc.contributor.authorTelk, Anna
dc.contributor.authorŻur, Iwona
dc.date.access2025-07-24
dc.date.accessioned2025-07-24T06:22:27Z
dc.date.available2025-07-24T06:22:27Z
dc.date.copyright2025-01-25
dc.date.issued2024
dc.description.abstract<jats:p>Among various methods stimulating biological progress, double haploid (DH) technology, which utilizes the process of microspore embryogenesis (ME), is potentially the most effective. However, the process depends on complex interactions between many genetic, physiological and environmental variables, and in many cases, e.g., winter wheat, does not operate with the efficiency required for commercial use. Stress associated with low-temperature treatment, isolation and transfer to in vitro culture has been shown to disturb redox homeostasis and generate relatively high levels of reactive oxygen species (ROS), affecting microspore vitality. The aim of this study was to investigate whether controlled plant growth, specific tiller pre-treatment and culture conditions could improve the potential of microspores to cope with stress and effectively induce ME. To understand the mechanism of the stress response, hydrogen peroxide levels, total activity and the content of the most important low-molecular-weight antioxidants (glutathione and ascorbate), as well as the content of selected macro- (Mg, Ca, NA, K) and micronutrients (Mn, Zn, Fe, Cu, Mo) were determined. These analyses, combined with the cytological characteristics of the microspore suspensions, allowed us to demonstrate that an increased microspore vitality and stronger response to ME induction were associated with higher stress resistance based on more efficient ROS scavenging and nutrient management. It was shown that a modified procedure, combining a low temperature with mannitol and sodium selenate tiller pre-treatment, reduced oxidative stress and improved the effectiveness of ME in winter wheat lines.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_research
dc.description.financecost12000,00
dc.description.if4,1
dc.description.number3
dc.description.points70
dc.description.versionfinal_published
dc.description.volume13
dc.identifier.doi10.3390/plants13030363
dc.identifier.issn2223-7747
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/3955
dc.identifier.weblinkhttps://www.mdpi.com/2223-7747/13/3/363
dc.languageen
dc.pbn.affiliationagriculture and horticulture
dc.relation.ispartofPlants
dc.relation.pagesart. 363
dc.rightsCC-BY
dc.sciencecloudsend
dc.subject.enmicrospore embryogenesis
dc.subject.enwinter wheat
dc.subject.enmacro- and micronutrients
dc.subject.enstress
dc.subject.enantioxidants
dc.subject.enhydrogen peroxide
dc.titleNew Prospects for Improving Microspore Embryogenesis Induction in Highly Recalcitrant Winter Wheat Lines
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue3
oaire.citation.volume13
project.funder.nameMinistry of Agriculture and Rural Development of the Republic of Poland (grant number 4; DHR.hn.802.1.2023) in the frame of Basic Research for Biological Progress in Plant Production