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Exposure to a choline-deficient diet during pregnancy and lactation alters the liver transcriptome profile in offspring of dams with fatty liver

cris.lastimport.scopus2025-10-23T06:59:48Z
cris.virtual.author-orcid0000-0002-4731-6243
cris.virtual.author-orcid0000-0002-2045-0709
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcidd951fe7e-3886-48a5-8661-b05d0c844622
cris.virtualsource.author-orcid20ac3fe1-f398-4ef7-ab0b-a6a20460f69f
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.abstract.enBackground & aims The developmental origin of health and disease hypothesis shows that early adverse exposures can have lifelong health effects. Thus, the aim of this study was to analyze the impact of choline intake during pregnancy and/or lactation on gene expression profiles in the liver of 24-day-old male rat offspring from dams with non-alcoholic fatty liver disease (NAFLD). Methods Phenotypic characteristic, histological examination and global transcriptome pattern of liver tissue specimens obtained from offspring of dams suffering from fatty liver, provided with proper choline intake during pregnancy and lactation (NN), fed a choline-deficient diet during both periods (DD), deprived of choline only during pregnancy (DN), or only during lactation (ND), was performed. The global gene expression profile was analyzed by using microarray approach (Affymetrix® Rat Gene 2.1 ST Array Strip). The relative expression of selected genes was validated by real-time polymerase chain reaction (qPCR). Results The histological examination of rat liver sections indicated alternations typical for fatty liver in all analyzed groups with increased progression among groups deprived of choline. Choline deficiency in the maternal diet was associated with changes in body mass and composition but not with biochemical marker levels, except for the high density lipoprotein fraction of cholesterol (HDL). Enhanced expression of genes involved in oxidative stress, cell proliferation, activation of catabolic processes related to hepatocyte dysfunction and cell membrane composition were simultaneously observed in all choline-deficient groups. Conclusions An adequate amount of choline in the diet of a mother with fatty liver during pregnancy and/or lactation can regulate gene expression in the offspring's liver and contribute to a milder stage of the disease in the progeny. Moreover, proper choline supply during the postpartum period is as crucial as during the prenatal period.
dc.affiliationWydział Nauk o Żywności i Żywieniu
dc.affiliation.instituteKatedra Żywienia Człowieka i Dietetyki
dc.contributor.authorMikołajczyk-Stecyna, Joanna
dc.contributor.authorZuk, Ewelina
dc.contributor.authorChmurzyńska, Agata
dc.contributor.authorBlatkiewicz, Malgorzata
dc.contributor.authorJopek, Karol
dc.contributor.authorRucinski, Marcin
dc.date.accessioned2025-03-18T09:50:39Z
dc.date.available2025-03-18T09:50:39Z
dc.date.issued2025
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if2,9
dc.description.numberApril 2025
dc.description.points40
dc.description.volume66
dc.identifier.doi10.1016/j.clnesp.2025.01.028
dc.identifier.issn2405-4577
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/2593
dc.languageen
dc.relation.ispartofClinical Nutrition ESPEN
dc.relation.pages9-23
dc.rightsClosedAccess
dc.sciencecloudsend
dc.subject.engene expression
dc.subject.enprenatal programming
dc.subject.enpostnatal programming
dc.subject.encholine
dc.subject.enNAFLD
dc.titleExposure to a choline-deficient diet during pregnancy and lactation alters the liver transcriptome profile in offspring of dams with fatty liver
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.volume66