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Effect of gastrointestinal digestion on the stability and cytotoxicity of conventional and pegylated liposomes encapsulated with stigmasterol and its esters

cris.virtual.author-orcid0000-0001-6343-332X
cris.virtual.author-orcid0000-0002-0529-3725
cris.virtual.author-orcid0000-0002-4385-0051
cris.virtual.author-orcid0000-0001-7706-4035
cris.virtual.author-orcid0000-0003-3708-2890
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cris.virtualsource.author-orcid307551d0-aa67-4ae6-b57b-fb099d8300e7
cris.virtualsource.author-orcida4043375-ad34-47db-b06a-c0b221cf8d5b
cris.virtualsource.author-orcid48ec8e95-930e-4be2-b47a-b597e5d7440b
cris.virtualsource.author-orcid349c35ce-c6ee-4d8a-9a7f-b3aace65dbd7
cris.virtualsource.author-orcida0e99de6-16e7-4a8e-955e-348a7bec4f41
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.abstract.enConventional and PEGylated liposomes encapsulated with stigmasterol and its esters were prepared, and their stability and cytotoxicity during in vitro gastrointestinal digestion were determined. The release of stigmasterol and its oxidation products were analyzed using GC-FID. The cytotoxicity before and after digestion was assessed using human normal small intestinal HIEC-6 cells and colon mucosa CCD 841CoN cells. Both liposome PEGylation and the chemical structure of the encapsulated compounds affect the stability and cytotoxicity of the liposomes after gastrointestinal digestion. Esterification of stigmasterol had an effect on the encapsulation of stigmasterol in liposomes, especially PEGylated liposomes, but the level of fatty acid saturation had no significant influence. PEGylation of liposomes did not inhibit sterol oxidation, but in fact led to the formation of oxidation derivatives. Gastrointestinal digestion increased the cytotoxicity of liposomes with stigmasterol (L-St), but liposomes encapsulated with stigmasterol esters had lower cytotoxicity than did undigested liposomes. PEGylation of liposomes did not cause an increase in cytotoxicity to the small intestinal or colon mucosa cells. However, it should be highlighted that the cytotoxic effects of the digested PEGylated liposomes were considerable higher in colon CCD 841CoN cells than in small intestinal HIEC-6 cells.
dc.affiliationWydział Nauk o Żywności i Żywieniu
dc.affiliation.instituteKatedra Technologii Żywności Pochodzenia Roślinnego
dc.affiliation.instituteKatedra Biotechnologii i Mikrobiologii Żywności
dc.contributor.authorRudzińska, Magdalena
dc.contributor.authorGrygier, Anna
dc.contributor.authorOlejnik, Anna
dc.contributor.authorKowalska, Katarzyna
dc.contributor.authorIgielska-Kalwat, Joanna
dc.contributor.authorKmiecik, Dominik
dc.contributor.authorCieślik-Boczula, Katarzyna
dc.date.access2025-09-30
dc.date.accessioned2025-10-02T05:50:09Z
dc.date.available2025-10-02T05:50:09Z
dc.date.copyright2025-09-01
dc.date.issued2025
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if3,9
dc.description.points140
dc.description.versionfinal_published
dc.description.volume15
dc.identifier.doi10.1038/s41598-025-17473-5
dc.identifier.issn2045-2322
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/5129
dc.identifier.weblinkhttps://www.nature.com/articles/s41598-025-17473-5
dc.languageen
dc.relation.ispartofScientific Reports
dc.relation.pagesart. 32079
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOPEN_JOURNAL
dc.titleEffect of gastrointestinal digestion on the stability and cytotoxicity of conventional and pegylated liposomes encapsulated with stigmasterol and its esters
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue1
oaire.citation.volume15