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The role of phoenixin in the proliferation and migration of ectopic epithelial cells in vitro

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cris.virtual.author-orcid0000-0002-2198-6046
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cris.virtualsource.author-orcid2068f136-150d-448d-a014-c5b41eb5cb60
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cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.abstract.enAim Endometriosis is one of the most common gynecologic diseases in women of reproductive age. The pathophysiology of endometriosis is still not fully understood. Phoenixin (PNX-14) is a newly discovered neuropeptide that regulates the hypothalamo–pituitary–gonadal (HPG) axis and reproductive functions. Recently, we reported that PNX-14, its precursor protein and receptor were expressed in human endometrium. Moreover, PNX-14 serum levels in endometriosis were reduced. This study aimed to evaluate the in vitro biological functions of physiological PNX-14 concentrations on the ectopic endometrium Z12 cells. Methods The proliferation and migration of Z12 cells were assessed using the xCELLigence® RTCA DP system following 72 h of stimulation with 0.05 and 0.2 nM of PNX-14. GPR173 and small integral membrane protein 20 (SMIM20) gene expression was evaluated using quantitative polymerase chain reaction (qPCR) and the protein levels of GPR173 were analyzed using Western blot analysis. Results PNX-14 at the concentration observed in the serum of patients with endometriosis (0.05 nM) reduced GPR173 and increased SMIM20 expression, while protein levels of GPR173 remained unchanged. Cell proliferation was increased by the 0.02 nM PNX-14— the concentration found in healthy subjects. The 0.2 nM of PNX-14 decreased SMIM20 expression with no change to GPR173 expression and reduced ectopic epithelial cell proliferation during the first 5 h after stimulation. However, at 72 h, the proliferation increased. Conclusions This study shows that PNX-14 at endometriosis specific concentration desensitized ectopic epithelium to PNX-14, and increased the expression of SMIM20 to restore the physiological levels of PNX-14.
dc.affiliationWydział Medycyny Weterynaryjnej i Nauk o Zwierzętach
dc.affiliation.instituteKatedra Fizjologii, Biochemii i Biostruktury Zwierząt
dc.contributor.authorKulinska, Karolina Iwona
dc.contributor.authorBiałas, Piotr
dc.contributor.authorDera-Szymanowska, Anna
dc.contributor.authorBillert, Maria
dc.contributor.authorKotwicka, Małgorzata
dc.contributor.authorSzymanowski, Krzysztof
dc.contributor.authorWołun-Cholewa, Maria
dc.date.access2025-06-06
dc.date.accessioned2025-09-04T09:12:39Z
dc.date.available2025-09-04T09:12:39Z
dc.date.copyright2023-01-20
dc.date.issued2023
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if2,5
dc.description.number26 February 2023
dc.description.points100
dc.description.versionfinal_published
dc.description.volume646
dc.identifier.doi10.1016/j.bbrc.2023.01.056
dc.identifier.issn0006-291X
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/4620
dc.identifier.weblinkhttps://www.sciencedirect.com/science/article/pii/S0006291X23000876?via%3Dihub
dc.languageen
dc.relation.ispartofBiochemical and Biophysical Research Communications
dc.relation.pages44-49
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOPEN_JOURNAL
dc.subject.enphoenixin
dc.subject.enendometriosis
dc.subject.enneuropeptides
dc.subject.enSMIM20
dc.subject.enGPR173
dc.subject.enmigration
dc.subject.enproliferation
dc.titleThe role of phoenixin in the proliferation and migration of ectopic epithelial cells in vitro
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.volume646