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Nesfatin-1 expression and blood plasma concentration in female dogs suffering from cystic endometrial hyperplasia and pyometra and its possible interaction with phoenixin-14

cris.virtual.author-orcid0000-0002-4563-3798
cris.virtual.author-orcid0000-0003-4059-1362
cris.virtual.author-orcid0000-0002-2198-6046
cris.virtual.author-orcid0000-0002-3651-0972
cris.virtual.author-orcid0000-0001-5046-7416
cris.virtual.author-orcid0000-0003-3489-1684
cris.virtual.author-orcid0000-0002-1820-5202
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
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cris.virtualsource.author-orcid2887b0c9-4f57-4235-a68f-057f9a3211f9
cris.virtualsource.author-orcid9118c61a-b066-4efc-a7af-486f9ebf9021
cris.virtualsource.author-orcid2068f136-150d-448d-a014-c5b41eb5cb60
cris.virtualsource.author-orcid652e209d-434a-47b3-b5fb-64c0aac5a84c
cris.virtualsource.author-orcid8cc943c4-fa09-421d-9e2b-84b33ec18db9
cris.virtualsource.author-orcid4ed4e453-28dc-48e8-864d-49bd28fdc14e
cris.virtualsource.author-orcid9fd4d04e-0912-425f-812f-aab2e3debe36
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid18eda7dc-c10c-4267-bed0-38a3cc91af5c
dc.abstract.enBackground Nesfatin-1 is a neuropeptide that regulates the hypothalamic-pituitary-gonadal axis and may play a role in uterus function. It is co-expressed with other peptides, such as phoenixin, which can influence sex hormone secretion. Our previous research has confirmed that phoenixin-14 is involved in the development of cystic endometrial hyperplasia (CEH) and pyometra in dogs. Therefore, based on the similarities and interactions between these neuropeptides, we hypothesized that nesfatin-1 might also regulate the reproductive system in dogs. This study aimed to determine the expression of nesfatin-1 and its interaction with phoenixin-14 in dogs with CEH or pyometra compared to healthy females, and concerning animals’ body condition score (BCS 4–5/9 vs. BCS > 5/9). Results The analysis of nesfatin-1 in the uterus of bitches consisted of qPCR, western blot and immunofluorescence assays, and ELISAs. The results showed significantly higher nesfatin-1 encoding gene, nucleobindin-2 mRNA (Nucb2) and nesfatin-1 protein expression in overweight females and those suffering from CEH or pyometra compared to healthy animals. The immunoreactivity of nesfatin-1 was elevated in the uteri of bitches with higher BCS > 5/9. Moreover, nesfatin-1 blood concentrations increased in all examined overweight bitches. In the case of phoenixin signals, we found opposite results, regardless of the female body condition score. Conclusion The etiology of CEH and pyometra are not fully known, although we have expanded the level of knowledge with respect to the possible interaction of nesfatin-1 and phoenixin in female dogs’ uteri. They interact oppositely. With increasing female body weight, the expression of nesfatin-1 in the uterus and its peripheral blood concentration increased. However, for female dogs affected by CEH and pyometra, a decreased level of phoenixin-14, irrespective of their body condition score is characteristic. This knowledge could be crucial in the development of biomarkers for these conditions, which may lead to earlier recognition.
dc.affiliationWydział Medycyny Weterynaryjnej i Nauk o Zwierzętach
dc.affiliation.instituteKatedra Nauk Przedklinicznych i Chorób Zakaźnych
dc.affiliation.instituteKatedra Fizjologii, Biochemii i Biostruktury Zwierząt
dc.affiliation.instituteKatedra Genetyki i Podstaw Hodowli Zwierząt​​
dc.affiliation.instituteKatedra Chorób Wewnętrznych i Diagnostyki
dc.contributor.authorRybska, Marta
dc.contributor.authorSkrzypski, Marek
dc.contributor.authorBillert, Maria
dc.contributor.authorWojciechowicz, Tatiana
dc.contributor.authorŁukomska, Anna
dc.contributor.authorPawlak, Piotr
dc.contributor.authorNowak, Tomasz
dc.contributor.authorPusiak, Karolina
dc.contributor.authorWąsowska, Barbara
dc.date.access2024-12-03
dc.date.accessioned2024-12-03T12:20:54Z
dc.date.available2024-12-03T12:20:54Z
dc.date.copyright2024-10-25
dc.date.issued2024
dc.description.abstract<jats:title>Abstract</jats:title><jats:sec> <jats:title>Background</jats:title> <jats:p>Nesfatin-1 is a neuropeptide that regulates the hypothalamic-pituitary-gonadal axis and may play a role in uterus function. It is co-expressed with other peptides, such as phoenixin, which can influence sex hormone secretion. Our previous research has confirmed that phoenixin-14 is involved in the development of cystic endometrial hyperplasia (CEH) and pyometra in dogs. Therefore, based on the similarities and interactions between these neuropeptides, we hypothesized that nesfatin-1 might also regulate the reproductive system in dogs. This study aimed to determine the expression of nesfatin-1 and its interaction with phoenixin-14 in dogs with CEH or pyometra compared to healthy females, and concerning animals’ body condition score (BCS 4–5/9 vs. BCS &gt; 5/9).</jats:p> </jats:sec><jats:sec> <jats:title>Results</jats:title> <jats:p>The analysis of nesfatin-1 in the uterus of bitches consisted of qPCR, western blot and immunofluorescence assays, and ELISAs. The results showed significantly higher nesfatin-1 encoding gene, nucleobindin-2 mRNA (<jats:italic>Nucb2</jats:italic>) and nesfatin-1 protein expression in overweight females and those suffering from CEH or pyometra compared to healthy animals. The immunoreactivity of nesfatin-1 was elevated in the uteri of bitches with higher BCS &gt; 5/9. Moreover, nesfatin-1 blood concentrations increased in all examined overweight bitches. In the case of phoenixin signals, we found opposite results, regardless of the female body condition score.</jats:p> </jats:sec><jats:sec> <jats:title>Conclusion</jats:title> <jats:p>The etiology of CEH and pyometra are not fully known, although we have expanded the level of knowledge with respect to the possible interaction of nesfatin-1 and phoenixin in female dogs’ uteri. They interact oppositely. With increasing female body weight, the expression of nesfatin-1 in the uterus and its peripheral blood concentration increased. However, for female dogs affected by CEH and pyometra, a decreased level of phoenixin-14, irrespective of their body condition score is characteristic. This knowledge could be crucial in the development of biomarkers for these conditions, which may lead to earlier recognition.</jats:p> </jats:sec>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if2,3
dc.description.points140
dc.description.versionfinal_published
dc.description.volume20
dc.identifier.doi10.1186/s12917-024-04336-w
dc.identifier.issn1746-6148
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/2158
dc.identifier.weblinkhttps://bmcvetres.biomedcentral.com/articles/10.1186/s12917-024-04336-w#Sec9
dc.languageen
dc.relation.ispartofBMC Veterinary Research
dc.relation.pagesart. 486
dc.rightsCC-BY
dc.sciencecloudsend
dc.share.typeOPEN_JOURNAL
dc.subject.ennesfatin-1
dc.subject.enphoenixin
dc.subject.enuterus
dc.subject.enreproductive diseases
dc.subject.encanine
dc.subject.enBCS
dc.titleNesfatin-1 expression and blood plasma concentration in female dogs suffering from cystic endometrial hyperplasia and pyometra and its possible interaction with phoenixin-14
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue1
oaire.citation.volume20