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Homocysteine thiolactone and other sulfur-containing amino acid metabolites are associated with fibrin clot properties and the risk of ischemic stroke

cris.virtual.author-orcid0000-0001-9218-385X
cris.virtual.author-orcid0000-0002-0791-5057
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cris.virtual.author-orcid0000-0001-5845-4409
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cris.virtualsource.author-orcid8086a6b6-8125-47c6-b14e-ac32562c73bd
cris.virtualsource.author-orcid06f046d1-df27-46e5-abe5-17d98aeed726
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cris.virtualsource.author-orcid4dde7a12-8c22-4e89-9d57-7c74b050ccc5
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dc.abstract.enHomocysteine (Hcy) and Hcy-thiolactone (HTL) afect fbrin clot properties and are linked to cardiovascular disease. Factors that infuence fbrin clot properties and stroke are not fully understood. To study sulfur-containing amino acid metabolites, fbrin clot lysis time (CLT) and maximum absorbance (Absmax) in relation to stroke, we analyzed plasma and urine from 191 stroke patients (45.0% women, age 68 ± 12 years) and 291 healthy individuals (59.7% women, age 50 ± 17 years). Plasma and urinary levels of sulfur-containing amino acid metabolites and fbrin clot properties were signifcantly diferent in stroke patients compared to healthy individuals. Fibrin CLT correlated with fbrin Absmax in healthy males (R2 = 0.439, P= 0.000), females (R2 = 0.245, P= 0.000), female stroke patients (R2 = 0.187, P= 0.000), but not in male stroke patients (R2 = 0.008, P=ns). Fibrin CLT correlated with age in healthy females but not males while fbrin Absmax correlated with age in both sexes; these correlations were absent in stroke patients. In multiple regression analysis in stroke patients, plasma (p)CysGly, pMet, and MTHFR A1298C polymorphism were associated with fbrin Absmax, while urinary (u)HTL, uCysGly, and pCysGly were signifcantly associated with fbrin CLT. In healthy individuals, uHTL and uGSH were signifcantly associated with fbrin Absmax, while pGSH, and CBS T833C 844ins68 polymorphism were associated with fbrin CLT. In logistic regression, uHTL, uHcy, pCysGly, pGSH, MTHFR C677T polymorphism, and Absmax were independently associated with stroke. Our fndings suggest that HTL and other sulfur-containing amino acid metabolites infuence fbrin clot properties and the risk of stroke.
dc.affiliationWydział Rolnictwa, Ogrodnictwa i Biotechnologii
dc.affiliation.instituteKatedra Biochemii i Biotechnologii
dc.contributor.authorSikora, Marta
dc.contributor.authorBretes, Ewa
dc.contributor.authorPerła-Kaján, Joanna
dc.contributor.authorUtyro, Olga
dc.contributor.authorBorowczyk, Kamila
dc.contributor.authorPiechocka, Justyna
dc.contributor.authorGłowacki, Rafał
dc.contributor.authorWojtasz, Izabela
dc.contributor.authorKaźmierski, Radosław
dc.contributor.authorJakubowski, Hieronim
dc.date.access2024-10-16
dc.date.accessioned2024-10-29T08:22:29Z
dc.date.available2024-10-29T08:22:29Z
dc.date.copyright2024-05-16
dc.date.issued2024
dc.description.abstract<jats:title>Abstract</jats:title><jats:p>Homocysteine (Hcy) and Hcy-thiolactone (HTL) affect fibrin clot properties and are linked to cardiovascular disease. Factors that influence fibrin clot properties and stroke are not fully understood. To study sulfur-containing amino acid metabolites, fibrin clot lysis time (CLT) and maximum absorbance (Abs<jats:sub>max</jats:sub>) in relation to stroke, we analyzed plasma and urine from 191 stroke patients (45.0% women, age 68 ± 12 years) and 291 healthy individuals (59.7% women, age 50 ± 17 years). Plasma and urinary levels of sulfur-containing amino acid metabolites and fibrin clot properties were significantly different in stroke patients compared to healthy individuals. Fibrin CLT correlated with fibrin Abs<jats:sub>max</jats:sub> in healthy males (R<jats:sup>2</jats:sup> = 0.439, <jats:italic>P</jats:italic> = 0.000), females (R<jats:sup>2</jats:sup> = 0.245, <jats:italic>P</jats:italic> = 0.000), female stroke patients (R<jats:sup>2</jats:sup> = 0.187, <jats:italic>P</jats:italic> = 0.000), but not in male stroke patients (R<jats:sup>2</jats:sup> = 0.008, <jats:italic>P</jats:italic> = ns). Fibrin CLT correlated with age in healthy females but not males while fibrin Abs<jats:sub>max</jats:sub> correlated with age in both sexes; these correlations were absent in stroke patients. In multiple regression analysis in stroke patients, plasma (p)CysGly, pMet, and <jats:italic>MTHFR A1298C</jats:italic> polymorphism were associated with fibrin Abs<jats:sub>max</jats:sub>, while urinary (u)HTL, uCysGly, and pCysGly were significantly associated with fibrin CLT. In healthy individuals, uHTL and uGSH were significantly associated with fibrin Abs<jats:sub>max</jats:sub>, while pGSH, and <jats:italic>CBS T833C 844ins68</jats:italic> polymorphism were associated with fibrin CLT. In logistic regression, uHTL, uHcy, pCysGly, pGSH, <jats:italic>MTHFR C677T</jats:italic> polymorphism, and Abs<jats:sub>max</jats:sub> were independently associated with stroke. Our findings suggest that HTL and other sulfur-containing amino acid metabolites influence fibrin clot properties and the risk of stroke.</jats:p>
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0.00
dc.description.if3,8
dc.description.points140
dc.description.versionfinal_published
dc.description.volume14
dc.identifier.doi10.1038/s41598-024-60706-2
dc.identifier.issn2045-2322
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/1947
dc.identifier.weblinkhttps://www.nature.com/articles/s41598-024-60706-2
dc.languageen
dc.relation.ispartofScientific Reports
dc.relation.pagesart. 11222
dc.rightsCC-BY
dc.sciencecloudsend
dc.share.typeOPEN_JOURNAL
dc.subject.enHomocysteine thiolactone
dc.subject.enSulfur amino acids
dc.subject.enFibrin clot properties
dc.subject.enStroke
dc.titleHomocysteine thiolactone and other sulfur-containing amino acid metabolites are associated with fibrin clot properties and the risk of ischemic stroke
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue1
oaire.citation.volume14