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  4. Plant Metabolites Affect Fusarium proliferatum Metabolism and In Vitro Fumonisin Biosynthesis
 
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Plant Metabolites Affect Fusarium proliferatum Metabolism and In Vitro Fumonisin Biosynthesis

Type
Journal article
Language
English
Date issued
2023
Author
Lalak-Kańczugowska, Justyna
Witaszak, Natalia
Waśkiewicz, Agnieszka 
Bocianowski, Jan 
Stępień, Łukasz
Faculty
Wydział Leśny i Technologii Drewna
Wydział Rolnictwa, Ogrodnictwa i Biotechnologii
Journal
International Journal of Molecular Sciences
ISSN
1661-6596
DOI
10.3390/ijms24033002
Web address
https://www.mdpi.com/1422-0067/24/3/3002
Volume
24
Number
3
Pages from-to
art. 3002
Abstract (EN)
Fusarium proliferatum is a common hemi-biotrophic pathogen that infect a wide range of host plants, often leading to substantial crop loss and yield reduction. F. proliferatum synthesizes various mycotoxins, and fumonisins B are the most prevalent. They act as virulence factors and specific effectors that elicit host resistance. The effects of selected plant metabolites on the metabolism of the F. proliferatum strain were analyzed in this study. Quercetin-3-glucoside (Q-3-Glc) and kaempferol-3-rutinoside (K-3-Rut) induced the pathogen’s growth, while DIMBOA, isorhamnetin-3-O-rutinoside (Iso-3-Rut), ferulic acid (FA), protodioscin, and neochlorogenic acid (NClA) inhibited fungal growth. The expression of seven F. proliferatum genes related to primary metabolism and four FUM genes was measured using RT-qPCR upon plant metabolite addition to liquid cultures. The expression of CPR6 and SSC1 genes was induced 24 h after the addition of chlorogenic acid (ClA), while DIMBOA and protodioscin reduced their expression. The transcription of FUM1 on the third day of the experiment was increased by all metabolites except for Q-3-Glc when compared to the control culture. The expression of FUM6 was induced by protodioscin, K-3-Rut, and ClA, while FA and DIMBOA inhibited its expression. FUM19 was induced by all metabolites except FA. The highest concentration of fumonisin B1 (FB1) in control culture was 6.21 µg/mL. Protodioscin did not affect the FB content, while DIMBOA delayed their synthesis/secretion. Flavonoids and phenolic acids displayed similar effects. The results suggest that sole metabolites can have lower impacts on pathogen metabolism and mycotoxin synthesis than when combined with other compounds present in plant extracts. These synergistic effects require additional studies to reveal the mechanisms behind them.
Keywords (EN)
  • Fusarium

  • gene expression

  • metabolites

  • mycotoxins

  • plant-pathogen interaction

  • qPCR

License
cc-bycc-by CC-BY - Attribution
Open access date
February 3, 2023
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