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Follicular-fluid extracellular vesicles support energy metabolism of bovine oocytes, improving blastocyst development and quality

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cris.virtual.author-orcid0000-0001-6384-791X
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cris.virtualsource.author-orcid02a0d8c3-755b-4ed1-a428-3e47baa39519
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dc.abstract.enExtracellular vesicles (EVs) from follicular fluid (FF) seem to play a significant role in communication within ovarian follicles in several species. The present study aimed to examine the supporting effect of FF-derived small EVs (FF-sEVs) during in vitro maturation (IVM) of bovine cumulus–oocyte complexes (COCs) under conditions of disturbed energy metabolism. Bovine COCs were matured in vitro with inhibitors targeting lipid metabolism (etomoxir) or glucose metabolism (iodoacetate combined with dehydroepiandrosterone), in the presence or absence of FF-sEVs. Following maturation, oocytes and cumulus cells were analyzed by real-time quantitative polymerase chain reaction (qPCR) and stained to visualize lipid droplets. The uptake of FF-sEVs was visualized by fluorescent labeling. In vitro fertilization and embryo culture were followed by mass spectrometry analysis of hatched blastocysts. We demonstrate for the first time that FF-sEVs are transported from the medium into the oocytes, via the cumulus cells and through transzonal projections into the perivitelline space and ooplasm. Cumulus cells under metabolic stress conditions exhibit an increased FF-sEV uptake from the maturation medium. FF-sEV supplementation during metabolic stress conditions enhances the MII rate in oocytes and positively affects subsequent embryo development and quality revealed by altered metabolic activity. Lipid droplet parameters and gene expression in cumulus cells and oocytes are affected by FF-sEV supplementation, which is more pronounced in cumulus cells. Our findings show that FF-sEV supplementation during IVM under metabolic stress conditions significantly affects COCs, with a positive effect on further blastocyst quality. We provide novel insights into the role of FF-sEVs in oocyte maturation and blastocyst development.
dc.affiliationWydział Medycyny Weterynaryjnej i Nauk o Zwierzętach
dc.affiliation.instituteKatedra Genetyki i Podstaw Hodowli Zwierząt​​
dc.contributor.authorLipinska, Paulina
dc.contributor.authorSmits, Katrien
dc.contributor.authorVan Soom, Ann
dc.contributor.authorPavani, Krishna Chaitanya
dc.contributor.authorWarzych-Plejer, Ewelina
dc.date.access2025-08-26
dc.date.accessioned2025-08-26T09:35:56Z
dc.date.available2025-08-26T09:35:56Z
dc.date.copyright2025-04-24
dc.date.issued2025
dc.description.abstract<jats:title>Abstract</jats:title> <jats:p>Extracellular vesicles (EVs) from follicular fluid (FF) seem to play a significant role in communication within ovarian follicles in several species. The present study aimed to examine the supporting effect of FF-derived small EVs (FF-sEVs) during in vitro maturation (IVM) of bovine cumulus–oocyte complexes (COCs) under conditions of disturbed energy metabolism. Bovine COCs were matured in vitro with inhibitors targeting lipid metabolism (etomoxir) or glucose metabolism (iodoacetate combined with dehydroepiandrosterone), in the presence or absence of FF-sEVs. Following maturation, oocytes and cumulus cells were analyzed by real-time quantitative polymerase chain reaction (qPCR) and stained to visualize lipid droplets. The uptake of FF-sEVs was visualized by fluorescent labeling. In vitro fertilization and embryo culture were followed by mass spectrometry analysis of hatched blastocysts. We demonstrate for the first time that FF-sEVs are transported from the medium into the oocytes, via the cumulus cells and through transzonal projections into the perivitelline space and ooplasm. Cumulus cells under metabolic stress conditions exhibit an increased FF-sEV uptake from the maturation medium. FF-sEV supplementation during metabolic stress conditions enhances the MII rate in oocytes and positively affects subsequent embryo development and quality revealed by altered metabolic activity. Lipid droplet parameters and gene expression in cumulus cells and oocytes are affected by FF-sEV supplementation, which is more pronounced in cumulus cells. Our findings show that FF-sEV supplementation during IVM under metabolic stress conditions significantly affects COCs, with a positive effect on further blastocyst quality. We provide novel insights into the role of FF-sEVs in oocyte maturation and blastocyst development.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_act
dc.description.financecost19735,20
dc.description.if3,0
dc.description.number1
dc.description.points200
dc.description.versionfinal_published
dc.description.volume113
dc.identifier.doi10.1093/biolre/ioaf096
dc.identifier.eissn1529-7268
dc.identifier.issn0006-3363
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/4369
dc.identifier.weblinkhttps://academic.oup.com/biolreprod/article/113/1/109/8119133
dc.languageen
dc.relation.ispartofBiology of Reproduction
dc.relation.pages109-126
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOTHER
dc.subject.enextracellular vesicles
dc.subject.enoocyte
dc.subject.enembryo
dc.subject.enenergy metabolism
dc.titleFollicular-fluid extracellular vesicles support energy metabolism of bovine oocytes, improving blastocyst development and quality
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue1
oaire.citation.volume113