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Prevalence and characterisation of antimicrobial resistance genes and class 1 and 2 integrons in multiresistant Escherichia coli isolated from poultry production

cris.virtual.author-orcid0000-0002-2572-0202
cris.virtual.author-orcid0000-0003-4608-6092
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cris.virtual.author-orcid0000-0002-9204-5534
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cris.virtual.author-orcid0000-0003-2833-5083
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cris.virtualsource.author-orcid4fbd1f59-0dc5-4c7a-8b19-33126b5d8e0c
cris.virtualsource.author-orcidc925cff9-7928-4326-9e3d-a4c04666d690
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cris.virtualsource.author-orcid9107a3d6-a60a-4353-ad9c-465de2211a83
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cris.virtualsource.author-orcid713c4db4-53f8-4862-ac61-6104db5e840c
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dc.abstract.enA global increase in the populations of drug resistant bacteria exerts negative effects on animal production and human health. Our study has been focused on the assessment of resistance determinants in relation to phenotypic resistance of the 74 commensal E. coli isolates present in different ecological environments. The samples were collected from poultry litter, feces, and neck skin. Among the microorganisms isolated from the poultry litter (group A), the highest resistance was noted against AMP and DOX (100%). In the E. coli extracts from the cloacal swabs (group B), the highest resistance was observed against AMP (100%) and CIP (92%). The meat samples (group C) were characterized by resistance to AMP (100%) and STX (94.7%). Genes encoding resistance to β-lactams (blaTEM, blaCTX-M), fluoroquinolones (qnrA, qnrB, qnrS), aminoglycosides (strA-strB, aphA1, aac(3)-II), sulfonamides (sul1, sul2, sul3), trimethoprim (dfr1, dfr5, dfr7/17) and tetracyclines (tetA, tetB) were detected in the studied bacterial isolates. The presence of class 1 and 2 integrons was confirmed in 75% of the MDR E. coli isolates (plasmid DNA), of which 60% contained class 1 integrons, 15% contained class 2 integrons, and 11.7% carried integrons of both classes. Thus, it may be concluded that integrons are the common mediators of antimicrobial resistance among commensal multidrug resistant Escherichia coli at important stages of poultry production.
dc.affiliationWydział Medycyny Weterynaryjnej i Nauk o Zwierzętach
dc.affiliation.instituteKatedra Hodowli Zwierząt i Oceny Surowców
dc.affiliation.instituteKatedra Chorób Wewnętrznych i Diagnostyki
dc.affiliation.instituteKatedra Genetyki i Podstaw Hodowli Zwierząt​​
dc.contributor.authorRacewicz, Przemysław Łukasz
dc.contributor.authorMajewski, Michał
dc.contributor.authorBiesiada, Hanna
dc.contributor.authorNowaczewski, Sebastian
dc.contributor.authorWilczyński, Jarosław
dc.contributor.authorWystalska, Danuta
dc.contributor.authorKubiak, Magdalena
dc.contributor.authorPszczoła, Marcin Jerzy
dc.contributor.authorMadeja, Zofia E.
dc.date.access2026-01-27
dc.date.accessioned2026-02-09T06:58:08Z
dc.date.available2026-02-09T06:58:08Z
dc.date.copyright2022-04-11
dc.date.issued2022
dc.description.abstract<jats:title>Abstract</jats:title><jats:p>A global increase in the populations of drug resistant bacteria exerts negative effects on animal production and human health. Our study has been focused on the assessment of resistance determinants in relation to phenotypic resistance of the 74 commensal <jats:italic>E. coli</jats:italic> isolates present in different ecological environments. The samples were collected from poultry litter, feces, and neck skin. Among the microorganisms isolated from the poultry litter (group A), the highest resistance was noted against AMP and DOX (100%). In the <jats:italic>E. coli</jats:italic> extracts from the cloacal swabs (group B), the highest resistance was observed against AMP (100%) and CIP (92%). The meat samples (group C) were characterized by resistance to AMP (100%) and STX (94.7%). Genes encoding resistance to β-lactams (<jats:italic>bla</jats:italic><jats:sub>TEM</jats:sub>, <jats:italic>bla</jats:italic><jats:sub>CTX-M</jats:sub>), fluoroquinolones (<jats:italic>qnrA, qnrB, qnrS</jats:italic>), aminoglycosides (<jats:italic>strA-strB, aphA1, aac(3)-II</jats:italic>), sulfonamides (<jats:italic>sul1, sul2, sul3</jats:italic>), trimethoprim (<jats:italic>dfr1, dfr5, dfr7/17</jats:italic>) and tetracyclines (<jats:italic>tetA, tetB</jats:italic>) were detected in the studied bacterial isolates. The presence of class 1 and 2 integrons was confirmed in 75% of the MDR <jats:italic>E. coli</jats:italic> isolates (plasmid DNA), of which 60% contained class 1 integrons, 15% contained class 2 integrons, and 11.7% carried integrons of both classes. Thus, it may be concluded that integrons are the common mediators of antimicrobial resistance among commensal multidrug resistant <jats:italic>Escherichia coli</jats:italic> at important stages of poultry production.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if4,6
dc.description.points140
dc.description.versionfinal_published
dc.description.volume12
dc.identifier.doi10.1038/s41598-022-09996-y
dc.identifier.issn2045-2322
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/7228
dc.identifier.weblinkhttps://www.nature.com/articles/s41598-022-09996-y
dc.languageen
dc.relation.ispartofScientific Reports
dc.relation.pagesart. 6062
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOPEN_JOURNAL
dc.titlePrevalence and characterisation of antimicrobial resistance genes and class 1 and 2 integrons in multiresistant Escherichia coli isolated from poultry production
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue1
oaire.citation.volume12