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Energy metabolism disorders during in vitro maturation of bovine cumulus-oocyte complexes interfere with blastocyst quality and metabolism

cris.virtual.author-orcid0000-0003-3489-1684
cris.virtual.author-orcid0000-0002-7642-0787
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cris.virtualsource.author-orcid4ed4e453-28dc-48e8-864d-49bd28fdc14e
cris.virtualsource.author-orcidfc57cf04-e023-4b05-9241-1c5d72ef234b
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cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.abstract.enGlucose and fatty acids (FA) metabolism disturbances during oocyte in vitro maturation (IVM) affect their metabolism and surrounding cumulus cells, but only inhibition of glucose metabolism decreases embryo culture efficiency. Therefore, the present experiment aimed to reveal if glucose or FA metabolism inhibition leads to the disruption of embryo developmental potential, and to characterize the metabolic landscape of embryos reaching the blastocyst stage. Inhibitors of glucose (IO + DHEA) or FA (ETOMOXIR) metabolism were applied during IVM, and the control group was matured under standard conditions. Blastocysts obtained from experimental and control groups were analyzed with regard to lipidome and metabolome (mass spectrometry), transcriptome (RNA-Seq) and fluorescence lipid droplets staining (BODIPY). We showed that inhibition of glucose and fatty acid metabolism leads to cellular stress response compromising the quality of preimplantation embryos. The inhibition of energy metabolism affects membrane fluidity as well as downregulates fatty acids biosynthesis and gene expression of trophectoderm cell line markers. Therefore, we conclude that oocyte maturation environment exerts a substantial effect on preimplantation development programming at cellular and molecular levels.
dc.affiliationWydział Medycyny Weterynaryjnej i Nauk o Zwierzętach
dc.affiliation.instituteKatedra Genetyki i Podstaw Hodowli Zwierząt​​
dc.contributor.authorPawlak, Piotr
dc.contributor.authorLipinska, Paulina
dc.contributor.authorSell-Kubiak, Ewa
dc.contributor.authorKajdasz, Arkadiusz
dc.contributor.authorDerebecka, Natalia
dc.contributor.authorWarzych, Ewelina
dc.date.accessioned2025-02-04T10:38:56Z
dc.date.available2025-02-04T10:38:56Z
dc.date.issued2024
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if2,5
dc.description.numberMay 2024
dc.description.points100
dc.description.volume509
dc.identifier.doi10.1016/j.ydbio.2024.02.004
dc.identifier.eissn1095-564X
dc.identifier.issn0012-1606
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/2435
dc.languageen
dc.relation.ispartofDevelopmental Biology
dc.relation.pages51-58
dc.rightsClosedAccess
dc.sciencecloudsend
dc.subject.enlipid metabolism
dc.subject.enin vitro maturation
dc.subject.encattle
dc.subject.enmetabolome
dc.subject.enlipidome
dc.subject.entranscriptome
dc.subject.enblastocyst quality
dc.titleEnergy metabolism disorders during in vitro maturation of bovine cumulus-oocyte complexes interfere with blastocyst quality and metabolism
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.volume509