Simple item page
 

Cereal leaf beetle‐associated bacteria enhance the survival of their host upon insecticide treatments and respond differently to insecticides with different modes of action

cris.lastimport.scopus2025-10-23T06:55:59Z
cris.virtual.author-orcid0000-0002-1806-0891
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid6a2f8857-003b-41ec-9112-0ef6941bfd06
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.abstract.enThe cereal leaf beetle (CLB, Oulema melanopus) is one of the major cereal pests. The effect of insecticides belonging to different chemical classes, with different mechanisms of action and the active substances' concentrations on the CLB bacterial microbiome, was investigated. Targeted metagenomic analysis of the V3–V4 regions of the 16S ribosomal gene was used to determine the composition of the CLB bacterial microbiome. Each of the insecticides caused a decrease in the abundance of bacteria of the genus Pantoea, and an increase in the abundance of bacteria of the genus Stenotrophomonas, Acinetobacter, compared to untreated insects. After cypermethrin application, a decrease in the relative abundance of bacteria of the genus Pseudomonas was noted. The dominant bacterial genera in cypermethrin-treated larvae were Lactococcus, Pantoea, while in insects exposed to chlorpyrifos or flonicamid it was Pseudomonas. Insecticide-treated larvae were characterized, on average, by higher biodiversity and richness of bacterial genera, compared to untreated insects. The depletion of CLB-associated bacteria resulted in a decrease in larval survival, especially after cypermethrin and chlorpyrifos treatments. The use of a metagenome-based functional prediction approach revealed a higher predicted function of bacterial acetyl-CoA C-acetyltransferase in flonicamid and chlorpyrifos-treated larvae and tRNA dimethyltransferase in cypermethrin-treated insects than in untreated insects.
dc.affiliationWydział Rolnictwa, Ogrodnictwa i Biotechnologii
dc.affiliation.instituteKatedra Metod Matematycznych i Statystycznych
dc.contributor.authorWielkopolan, Beata
dc.contributor.authorSzabelska-Beręsewicz, Alicja
dc.contributor.authorGawor, Jan
dc.contributor.authorObrępalska‐Stęplowska, Aleksandra
dc.date.access2025-04-23
dc.date.accessioned2025-07-17T11:50:39Z
dc.date.available2025-07-17T11:50:39Z
dc.date.copyright2024-03
dc.date.issued2024
dc.description.abstract<jats:title>Abstract</jats:title><jats:p>The cereal leaf beetle (CLB, <jats:italic>Oulema melanopus</jats:italic>) is one of the major cereal pests. The effect of insecticides belonging to different chemical classes, with different mechanisms of action and the active substances' concentrations on the CLB bacterial microbiome, was investigated. Targeted metagenomic analysis of the V3–V4 regions of the 16S ribosomal gene was used to determine the composition of the CLB bacterial microbiome. Each of the insecticides caused a decrease in the abundance of bacteria of the genus <jats:italic>Pantoea</jats:italic>, and an increase in the abundance of bacteria of the genus <jats:italic>Stenotrophomonas</jats:italic>, <jats:italic>Acinetobacter</jats:italic>, compared to untreated insects. After cypermethrin application, a decrease in the relative abundance of bacteria of the genus <jats:italic>Pseudomonas</jats:italic> was noted. The dominant bacterial genera in cypermethrin‐treated larvae were <jats:italic>Lactococcus, Pantoea</jats:italic>, while in insects exposed to chlorpyrifos or flonicamid it was <jats:italic>Pseudomonas</jats:italic>. Insecticide‐treated larvae were characterized, on average, by higher biodiversity and richness of bacterial genera, compared to untreated insects. The depletion of CLB‐associated bacteria resulted in a decrease in larval survival, especially after cypermethrin and chlorpyrifos treatments. The use of a metagenome‐based functional prediction approach revealed a higher predicted function of bacterial acetyl‐CoA C‐acetyltransferase in flonicamid and chlorpyrifos‐treated larvae and tRNA dimethyltransferase in cypermethrin‐treated insects than in untreated insects.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if2,7
dc.description.number2
dc.description.points100
dc.description.versionfinal_published
dc.description.volume16
dc.identifier.doi10.1111/1758-2229.13247
dc.identifier.issn1758-2229
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/3892
dc.identifier.weblinkhttps://enviromicro-journals.onlinelibrary.wiley.com/doi/full/10.1111/1758-2229.13247
dc.languageen
dc.relation.ispartofEnvironmental Microbiology Reports
dc.relation.pagese13247
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOPEN_JOURNAL
dc.titleCereal leaf beetle‐associated bacteria enhance the survival of their host upon insecticide treatments and respond differently to insecticides with different modes of action
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue2
oaire.citation.volume16