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Effects of a Multi-Strain Lactic and Propionic Acid Bacteria Inoculant on Silage Quality, Methane Emissions, Milk Composition, and Rumen Microbiome

cris.lastimport.scopus2025-10-23T06:56:01Z
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dc.abstract.enEnsiling grass with microbial inoculants is a promising strategy to enhance forage quality, animal performance, and environmental sustainability. This study evaluated the effects of a multi-strain inoculant (Lactobacillus plantarum, L. buchneri, Propionibacterium acidipropionici, and P. thoeni) on silage fermentation, nutrient digestibility, milk production, methane emissions, and rumen microbiota in dairy cows. In a 2 × 2 crossover design, 24 lactating Polish Holstein–Friesians were fed total mixed rations differing only in grass silage treated with or without inoculant. Inoculated silage had lower pH (4.56 vs. 5.06; p = 0.02) and higher crude protein (129 vs. 111 g/kgDM; p < 0.05). Cows fed inoculated silage showed higher ruminal propionate (28.3 vs. 26.3 mM; p = 0.03), reduced ammonia (7.61 vs. 8.67 mM; p = 0.02), and fewer protozoa (1.21 vs. 1.66 × 105/mL; p = 0.03). Nutrient digestibility improved (p < 0.05), while methane emissions declined both per cow (368 vs. 397 g/d; p = 0.01) and per kgDMI (15.1 vs. 16.5; p = 0.01). Milk yield increased (p = 0.04), and the fatty acid profile improved. Our study revealed that cows fed inoculated silage had higher nutrient digestibility, lower methane emissions, and microbial shifts in the rumen detected by 16S rRNA sequencing (p < 0.05).
dc.affiliationWydział Medycyny Weterynaryjnej i Nauk o Zwierzętach
dc.affiliation.instituteKatedra Żywienia Zwierząt
dc.affiliation.instituteKatedra Genetyki i Podstaw Hodowli Zwierząt​​
dc.affiliation.instituteKatedra Hodowli Zwierząt i Oceny Surowców
dc.contributor.authorOlorunlowu, Segun
dc.contributor.authorSidoruk, Pola
dc.contributor.authorSznajder, Julia
dc.contributor.authorSzczesny, Jakub
dc.contributor.authorCieślak, Dorota Marta
dc.contributor.authorPawlak, Piotr
dc.contributor.authorRyczek, Marcin
dc.contributor.authorHuang, Haihao
dc.contributor.authorLi, Lingyan
dc.contributor.authorIrawan, Agung
dc.contributor.authorKomisarek, Jolanta
dc.contributor.authorSzumacher, Małgorzata
dc.contributor.authorCieślak, Adam
dc.date.access2025-10-02
dc.date.accessioned2025-10-02T12:12:49Z
dc.date.available2025-10-02T12:12:49Z
dc.date.copyright2025-09-19
dc.date.issued2025
dc.description.abstract<jats:p>Ensiling grass with microbial inoculants is a promising strategy to enhance forage quality, animal performance, and environmental sustainability. This study evaluated the effects of a multi-strain inoculant (Lactobacillus plantarum, L. buchneri, Propionibacterium acidipropionici, and P. thoeni) on silage fermentation, nutrient digestibility, milk production, methane emissions, and rumen microbiota in dairy cows. In a 2 × 2 crossover design, 24 lactating Polish Holstein–Friesians were fed total mixed rations differing only in grass silage treated with or without inoculant. Inoculated silage had lower pH (4.56 vs. 5.06; p = 0.02) and higher crude protein (129 vs. 111 g/kgDM; p &lt; 0.05). Cows fed inoculated silage showed higher ruminal propionate (28.3 vs. 26.3 mM; p = 0.03), reduced ammonia (7.61 vs. 8.67 mM; p = 0.02), and fewer protozoa (1.21 vs. 1.66 × 105/mL; p = 0.03). Nutrient digestibility improved (p &lt; 0.05), while methane emissions declined both per cow (368 vs. 397 g/d; p = 0.01) and per kgDMI (15.1 vs. 16.5; p = 0.01). Milk yield increased (p = 0.04), and the fatty acid profile improved. Our study revealed that cows fed inoculated silage had higher nutrient digestibility, lower methane emissions, and microbial shifts in the rumen detected by 16S rRNA sequencing (p &lt; 0.05).</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financeother
dc.description.financecost5351,39
dc.description.if2,7
dc.description.number18
dc.description.points100
dc.description.versionfinal_published
dc.description.volume15
dc.identifier.doi10.3390/ani15182740
dc.identifier.issn2076-2615
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/5154
dc.identifier.weblinkhttps://www.mdpi.com/2076-2615/15/18/2740
dc.languageen
dc.relation.ispartofAnimals
dc.relation.pagesart. 2740
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOPEN_JOURNAL
dc.subject.ensilage
dc.subject.enfermentation quality
dc.subject.enrumen fermentation
dc.subject.enlactation performance
dc.subject.enmethane emission
dc.subject.enfatty acid
dc.titleEffects of a Multi-Strain Lactic and Propionic Acid Bacteria Inoculant on Silage Quality, Methane Emissions, Milk Composition, and Rumen Microbiome
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue18
oaire.citation.volume15
project.funder.nameThis study was supported by European Union’s Horizon 2020 Research and Innovation Program under Grant Agreement No. 696356 for research carried out within the ERA-GAS/ERA-NET SUSAN/ICT-AGRI project CCC farming (SUSAN/II/CCCFARMING/03/202); National Science Center, Poland (Grant No. 267659/7/NCBR/2015), and by statutory funding from the Faculty of Veterinary Medicine and Animal Science, Poznań University of Life Sciences (PULS), by the Department of Animal Nutrition (No. 506.533.04.00); Polish Minister of Science and Higher Education as part of the Strategy of the Poznan University of Life Sciences for 2024–2026 in the field of improving scientific research and development work in priority research areas.