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The genotoxic effect of morpholinium-based ionic liquids on Pseudomonas aeruginosa LES B58: electrochemical and in silico mechanistic study

cris.virtual.author-orcid0000-0002-9498-819X
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid0000-0003-3455-3044
cris.virtualsource.author-orcidd9f431b5-a0b9-488d-959c-edc054dfb309
cris.virtualsource.author-orcid8da63665-4835-4e83-a6d3-28544e67b2b4
cris.virtualsource.author-orcid789ea30a-f0af-454d-b257-827c734e8291
dc.abstract.enOne of the most relevant and persistent human pathogens is Pseudomonas aeruginosa. Among numerous antimicrobial agents examined for their ability to combat bacterial resistance are ionic liquids. Our study investigates the genotoxic effect of two ionic liquids which were previously documented to exhibit anti-biofilm and sensitizing activity towards P. aeruginosa: 4,4-didecylmorpholinium and 4-decyl-4-ethylmorpholinium 2,4-dichlorophenoxyacetate. To assess the effect of both ionic liquids on bacterial DNA we investigated changes in electrochemical signal of P. aeruginosa DNA isolated after bacteria exposure to tested compounds. We resulted in raised electrochemical signals of dsDNA after the exposition for 4-decyl-4-ethylmorpholinium, which may shown initial DNA destabilisation before occurring oxidative damage. The mechanism of potential interaction was verified using ab initio calculation of interaction with DNA base pairs: AT, GC and 8-oxoGC, and resulted in ultrahigh binding energy and interaction energy between 4-decyl-4-ethylmorpholinium and 8-oxoGC.
dc.affiliationWydział Rolnictwa, Ogrodnictwa i Biotechnologii
dc.affiliation.instituteKatedra Biochemii i Biotechnologii
dc.contributor.authorSzczepaniak, Oskar
dc.contributor.authorMichalski, Jakub
dc.contributor.authorNarożna, Dorota
dc.contributor.institutionUniwersytet Przyrodniczy w Poznaniu
dc.date.access2026
dc.date.accessioned2026-01-13T09:47:09Z
dc.date.available2026-01-13T09:47:09Z
dc.date.copyright2026-01-08
dc.date.issued2026
dc.description.accesstimebefore_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.versionfinal_published
dc.identifier.doi10.1016/j.sbsr.2026.100954
dc.identifier.issn2214-1804
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/6773
dc.identifier.weblinkhttps://www.sciencedirect.com/science/article/pii/S2214180426000048
dc.languageen
dc.pbn.affiliationbiotechnology
dc.pbn.affiliationagriculture and horticulture
dc.relation.ispartofSensing and Bio-Sensing Research
dc.relation.pagesart. 100954
dc.rightsCC-BY-NC-ND
dc.sciencecloudnosend
dc.share.typeOPEN_JOURNAL
dc.subject.enPseudomonas aeruginosa
dc.subject.enionic liquids
dc.subject.engenotoxicity
dc.subject.ensquare-wave voltammetry
dc.subject.enab initio
dc.subtypeArticleEarlyAccess
dc.titleThe genotoxic effect of morpholinium-based ionic liquids on Pseudomonas aeruginosa LES B58: electrochemical and in silico mechanistic study
dc.typeJournalArticle
dspace.entity.typePublication