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Red Clover Isoflavones as Effective Longevity Agents for Anti-Aging and Regenerative Skin Applications

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cris.virtual.author-orcid0000-0002-9670-3231
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cris.virtualsource.author-orcidd5bc5072-424b-49a4-92ce-2da11fa5d021
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dc.abstract.enRed clover (Trifolium pratense L.) is a rich source of isoflavones with documented antioxidant and skin-protective properties, yet substantial differences in phytochemical composition exist among cultivars. In this study, fourteen T. pratense cultivars were compared with respect to formononetin and biochanin A contents (Milena, Pasieka, Pyza, Milvus, Nemaro, Maro, Larus, Hammon, Vesna, Fregata, Carbo, Forelia, Osimia, and Elanus), and the relationship between isoflavone profiles and skin-related biological activity was evaluated. High-performance liquid chromatography revealed pronounced cultivar-dependent variability with formononetin and biochanin A contents ranging from 1.60 to 7.80 mg/g DW and from 0.69 to 6.44 mg/g DW, respectively. The observed variability was further visualized by principal component analysis. The cultivar with the highest total isoflavone content—Hammon, was selected for biological assessment. Its extract exhibited antioxidant (DPPH IC50 = 0.619 mg/mL; FRAP IC0.5 = 0.302 mg/mL) and enzyme inhibitory activities (elastase IC50 = 0.602 mg/mL, hyaluronidase IC50 = 22.44 mg/mL), and it significantly enhanced fibroblast migration in an in vitro scratch assay, indicating anti-aging and regenerative potential. These results demonstrate that red clover cultivars differ significantly in their suitability as sources of bioactive isoflavones and highlight the importance of cultivar selection for the development of standardized plant-derived anti-aging ingredients. However, it is worth emphasizing that isoflavones derived from red clover are a valuable group of active compounds with significant potential for topical application as anti-aging and regenerative agents, warranting further formulation development and in vivo validation.
dc.affiliationWydział Rolnictwa, Ogrodnictwa i Biotechnologii
dc.affiliation.instituteKatedra Agronomii
dc.contributor.authorGościniak, Anna
dc.contributor.authorBogusławska, Klaudyna
dc.contributor.authorSzymańska, Małgorzata
dc.contributor.authorKaproń-Plech, Barbara
dc.contributor.authorTrzaskoma, Piotr
dc.contributor.authorSzulc, Piotr
dc.contributor.authorCielecka-Piontek, Judyta
dc.date.access2026-02-11
dc.date.accessioned2026-02-19T08:38:42Z
dc.date.available2026-02-19T08:38:42Z
dc.date.copyright2026-02-10
dc.date.issued2026
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if4,1
dc.description.number4
dc.description.points70
dc.description.versionfinal_published
dc.description.volume15
dc.identifier.doi10.3390/plants15040553
dc.identifier.issn2223-7747
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/7390
dc.identifier.weblinkwww.mdpi.com/2223-7747/15/4/553
dc.languageen
dc.relation.ispartofPlants
dc.relation.pagesart. 553
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOPEN_JOURNAL
dc.subject.enTrifolium pratense L.
dc.subject.enisoflavones
dc.subject.enanti-aging
dc.subject.enskin regeneration
dc.subject.enlongevity
dc.titleRed Clover Isoflavones as Effective Longevity Agents for Anti-Aging and Regenerative Skin Applications
dc.title.volumeSpecial Issue Antimicrobial, Enzyme Inhibitory and Wound-Healing Properties of Plant Extracts and Phytochemicals
dc.typeJournalArticle
dspace.entity.typePublication