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In vitro modeling of endometriosis and endometriotic microenvironment – Challenges and recent advances

cris.virtual.author-orcid0000-0003-2725-0467
cris.virtual.author-orcid0000-0002-4385-0051
cris.virtualsource.author-orcidd077b699-93dd-4a9f-804f-4c575429c392
cris.virtualsource.author-orcid48ec8e95-930e-4be2-b47a-b597e5d7440b
dc.abstract.enEndometriosis is a chronic condition with high prevalence in reproductive age women, defined as the growth of endometrial tissue outside the uterine cavity, most commonly on the pelvic peritoneum. The ectopic endometrial lesions exist in a unique microenvironment created by the interaction of epithelial, stromal, endothelial, glandular, and immune cell components, dominated by inflammatory, angiogenic, and endocrine signals. Current research is directed at understanding the complex microenvironment of the lesions and its relationship with different endometriosis stages, phenotypes, and disease symptoms and at the development of novel diagnostic and therapeutic concepts that minimalize the undesirable side effects of current medical management. Recreating pathophysiological cellular and molecular mechanisms and identifying clinically relevant metrics to assess drug efficacy is a great challenge for the experimental disease models. This review summarizes the complete range of available in vitro experimental systems used in endometriotic studies, which reflect the multifactorial nature of the endometriotic lesion. The article discusses the simplistic in vitro models such as primary endometrial cells and endometriotic cell lines to heterogeneous 2D co-cultures, and recently more common, 3D systems based on self-organization and controlled assembly, both in microfluidic or bioprinting methodologies. Basic research models allow studying fundamental pathological mechanisms by which menstrual endometrium adheres, invades, and establishes lesions in ectopic sites. The advanced endometriosis experimental models address the critical challenges and unsolved problems and provide an approach to drug screening and medicine discovery by mimicking the complicated behaviors of the endometriotic lesion.
dc.affiliationWydział Nauk o Żywności i Żywieniu
dc.affiliation.instituteKatedra Biotechnologii i Mikrobiologii Żywności
dc.contributor.authorGołąbek-Grenda, Agata
dc.contributor.authorOlejnik, Anna
dc.date.access2025-11-17
dc.date.accessioned2025-11-17T08:04:50Z
dc.date.available2025-11-17T08:04:50Z
dc.date.copyright2022-06-09
dc.date.issued2022
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if4,8
dc.description.numberSeptember 2022
dc.description.points100
dc.description.reviewreview
dc.description.versionfinal_published
dc.description.volume97
dc.identifier.doi10.1016/j.cellsig.2022.110375
dc.identifier.issn0898-6568
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/5913
dc.identifier.weblinkhttps://www.sciencedirect.com/science/article/pii/S0898656822001371?via%3Dihub
dc.languageen
dc.relation.ispartofCellular Signalling
dc.relation.pagesart. 110375
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOTHER
dc.subject.enendometriotic niche
dc.subject.en2D and 3D
dc.subject.enendometriosis models
dc.subject.enspheroids
dc.subject.enorganoids
dc.subject.enmicrofluidics
dc.subject.enbioprinting
dc.titleIn vitro modeling of endometriosis and endometriotic microenvironment – Challenges and recent advances
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.volume97