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Assessment of the lysis efficiency of selected guanidinium thiocyanate/hydrochloride lysis buffers commonly used in PCR diagnostics

cris.lastimport.scopus2025-10-23T06:55:53Z
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cris.virtual.author-orcid0000-0003-4057-7333
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cris.virtualsource.author-orcidedaa3f24-c687-4516-93a8-b178493c02f0
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dc.abstract.enIn this study, the virucidal activities of Qiagen Buffer AL, Qiagen Buffer AVL and Roche MPLB (MagNA Pure lysis/binding) buffer, each containing a guanidine-based denaturing agent, were assessed against selected pathogenic animal (canine adenovirus type 2 (CAV-2), 6.0 log10 TCID50/mL and canine coronavirus (CCoV), 3.9 log10 TCID50/mL) and human (hepatitis A virus (HAV), 6.7 log10 TCID50/mL) viruses at different temperatures and over different times. In the virus inactivation experiments, all three lysis buffers were able to inactivate CAV-2 and CCoV even in a short 1-min contact time. Although the HAV titre was reduced by at least 4.5 log10, it was still observed to have residual infectivity. Only the AL lysis buffer in conjunction with heat treatment appeared to be highly efficient at HAV inactivation. A complete (99.99 %) inactivation of CAV-2 (6.0 log10 TCID50/mL) and CCoV (4.7 log10 TCID50/mL) by the MPLB buffer was also observed even at lower-than-recommended buffer concentrations. Generally, all lysis buffers were effective in the inactivation of enveloped and non-enveloped animal viruses. However, they reduced HAV infectivity to a lesser extent, indicating a need for a more stringent inactivation method to destroy infectivity of this virus in diagnostic material.
dc.affiliationWydział Medycyny Weterynaryjnej i Nauk o Zwierzętach
dc.affiliation.instituteKatedra Nauk Przedklinicznych i Chorób Zakaźnych
dc.contributor.authorKaupke, A.
dc.contributor.authorKwit, E.
dc.contributor.authorBigoraj, E.
dc.contributor.authorRadko, Lidia
dc.contributor.authorSpiess, K.
dc.contributor.authorRzeżutka, A.
dc.date.accessioned2025-05-06T10:29:13Z
dc.date.available2025-05-06T10:29:13Z
dc.date.issued2025
dc.description.bibliographybibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if2,2
dc.description.numberMay 2025
dc.description.points100
dc.description.volume187
dc.identifier.doi10.1016/j.rvsc.2025.105567
dc.identifier.eissn1532-2661
dc.identifier.issn0034-5288
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/2746
dc.languageen
dc.pbn.affiliationveterinary science
dc.relation.ispartofResearch in Veterinary Science
dc.relation.pagesart. 105567
dc.rightsClosedAccess
dc.sciencecloudsend
dc.subject.encanine adenovirus
dc.subject.encanine coronavirus
dc.subject.enhepatitis A virus
dc.subject.enlysis buffer
dc.subject.envirus inactivation
dc.subject.envirucidal activity
dc.titleAssessment of the lysis efficiency of selected guanidinium thiocyanate/hydrochloride lysis buffers commonly used in PCR diagnostics
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.volume187