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Development and application of duplex and triplex assays for simultaneous detection of resistance genes to leaf rust, Fusarium head blight, powdery mildew, Septoria tritici blotch, eyspot, stem rust and yellow rust in wheat

cris.virtual.author-orcid0000-0001-8208-2801
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cris.virtual.author-orcid0000-0001-9516-8911
cris.virtual.author-orcid0000-0001-9442-3124
cris.virtualsource.author-orcida9b12673-f948-47a8-868e-383feced1281
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cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
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cris.virtualsource.author-orcid2faa4bbb-a129-4bec-8137-bd8eeedf40e0
cris.virtualsource.author-orcide8127d2e-9d17-4f80-89ba-4750ab4c308d
dc.abstract.enResistance breeding is a widely promoted strategy for minimizing yield losses in wheat caused by various fungal diseases, including leaf rust (Puccinia triticina; Lr genes), Fusarium head blight (Fusarium spp.; Fhb), powdery mildew (Blumeria graminis; Pm), Septoria tritici blotch (Septoria tritici; Stb), eyespot (Oculimacula spp.; Pch—previously known as Pseudocercosporella herpotrichoides), stem rust (Puccinia graminis f. sp. tritici; Sr) and yellow rust (Puccinia striiformis; Yr). Understanding the prevalence of resistance genes in currently cultivated European varieties is crucial for their effective utilization in breeding programs. In this study, we developed 11 duplex and 13 triplex PCR assays for the simultaneous identification of diverse allelic combinations of disease resistance genes. Selected assays were used to analyze 70 European wheat varieties for the presence of 15 molecular markers associated with 12 resistance genes. The analyses identified diverse resistance gene combinations. The optimized multiplex PCR methods significantly reduce cost and time of analysis, making them valuable tools for marker-assisted selection (MAS) in wheat breeding programs.
dc.affiliationWydział Rolnictwa, Ogrodnictwa i Biotechnologii
dc.affiliation.instituteKatedra Genetyki i Hodowli Roślin
dc.contributor.authorBobrowska, Roksana
dc.contributor.authorMoskalik, Jakub
dc.contributor.authorNoweiska, Aleksandra
dc.contributor.authorSpychała, Julia
dc.contributor.authorTomkowiak, Agnieszka
dc.contributor.authorKwiatek, Michał Tomasz
dc.date.access2025-10-03
dc.date.accessioned2025-10-03T12:33:43Z
dc.date.available2025-10-03T12:33:43Z
dc.date.copyright2025-09-13
dc.date.issued2025
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.versionfinal_published
dc.identifier.doi10.1007/s13353-025-01004-z
dc.identifier.eissn2190-3883
dc.identifier.issn1234-1983
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/5182
dc.identifier.weblinkhttps://link.springer.com/article/10.1007/s13353-025-01004-z
dc.languageen
dc.pbn.affiliationagriculture and horticulture
dc.relation.ispartofJournal of Applied Genetics
dc.rightsCC-BY-NC-ND
dc.sciencecloudnosend
dc.share.typeOTHER
dc.subject.endiseases
dc.subject.enmolecular markers
dc.subject.enmultiplex PCR
dc.subject.enresistance breeding
dc.subject.enwheat
dc.subtypeArticleEarlyAccess
dc.titleDevelopment and application of duplex and triplex assays for simultaneous detection of resistance genes to leaf rust, Fusarium head blight, powdery mildew, Septoria tritici blotch, eyspot, stem rust and yellow rust in wheat
dc.typeJournalArticle
dspace.entity.typePublication