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Differential expression profile between amygdala and blood during chronic lithium treatment in a rat model of depression – a pilot study

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cris.virtual.author-orcid0000-0002-7182-6905
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dc.abstract.enLithium is a mood stabilizer widely used in the pharmacotherapy of bipolar disorder and treatment resistant depression. Taking into account dysregulated inflammatory activity in depression and the immunomodulatory role of lithium, we hypothesized that genes associated with inflammatory responses may be potential biomarkers of lithium action. We aimed to compare gene expression changes between the brain and the periphery after chronic lithium administration in an animal model of depression. Depressive behavior was induced by chronic mild stress protocol for 4 weeks. After 2 weeks, rats started to receive lithium (study group) or water (reference group). The control group were rats not exposed to stress. Amygdala, hippocampus, frontal cortex and peripheral blood were analyzed using whole transcriptome expression microarrays. Changes were confirmed with qPCR and ELISA assay. After 2 weeks of lithium administration, we observed significant changes in gene expression between amygdala and peripheral blood. Logistic regression analysis determined Alox15 expression as a predictor of lithium status, as its expression was tissue specific and increased in amygdala and decreased in blood. Analysis of serum ALOX15 protein revealed its upregulation after two week lithium administration. Our study suggests that lithium may have therapeutic potential in depressive behaviors. These results indicate immunomodulatory effect of lithium and that Alox15 may be a new potential marker of chronic lithium treatment.
dc.affiliationWydział Medycyny Weterynaryjnej i Nauk o Zwierzętach
dc.affiliation.instituteKatedra Fizjologii, Biochemii i Biostruktury Zwierząt
dc.contributor.authorSakrajda, Kosma
dc.contributor.authorSzczepankiewicz, Dawid
dc.contributor.authorNowakowska, Joanna
dc.contributor.authorCelichowski, Piotr
dc.contributor.authorBanach, Ewa
dc.contributor.authorZakowicz, Przemysław
dc.contributor.authorKołodziejski, Paweł
dc.contributor.authorPruszyńska-Oszmałek, Ewa
dc.contributor.authorPawlak, Joanna
dc.contributor.authorSzczepankiewicz, Aleksandra
dc.date.access2025-12-04
dc.date.accessioned2025-12-05T09:56:40Z
dc.date.available2025-12-05T09:56:40Z
dc.date.copyright2022-09-30
dc.date.issued2022
dc.description.abstract<jats:p>Lithium is a mood stabilizer widely used in the pharmacotherapy of bipolar disorder and treatment‑resistant depression. Taking into account dysregulated inflammatory activity in depression and the immunomodulatory role of lithium, we hypothesized that genes associated with inflammatory responses may be potential biomarkers of lithium action. We aimed to compare gene expression changes between the brain and the periphery after chronic lithium administration in an animal model of depression. Depressive behavior was induced by chronic mild stress protocol for 4 weeks. After 2 weeks, rats started to receive lithium (study group) or water (reference group). The control group were rats not exposed to stress. Amygdala, hippocampus, frontal cortex and peripheral blood were analyzed using whole transcriptome expression microarrays. Changes were confirmed with qPCR and ELISA assay. After 2 weeks of lithium administration, we observed significant changes in gene expression between amygdala and peripheral blood. Logistic regression analysis determined Alox15 expression as a predictor of lithium status, as its expression was tissue‑specific and increased in amygdala and decreased in blood. Analysis of serum ALOX15 protein revealed its upregulation after two‑week lithium administration. Our study suggests that lithium may have therapeutic potential in depressive behaviors. These results indicate immunomodulatory effect of lithium and that Alox15 may be a new potential marker of chronic lithium treatment.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if1,4
dc.description.number3
dc.description.points100
dc.description.versionfinal_published
dc.description.volume82
dc.identifier.doi10.55782/ane-2022-023
dc.identifier.eissn1689-0035
dc.identifier.issn0065-1400
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/6235
dc.identifier.weblinkhttps://ane.pl/index.php/ane/article/view/2345
dc.languageen
dc.relation.ispartofActa Neurobiologiae Experimentalis
dc.relation.pages245-253
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOPEN_JOURNAL
dc.subject.endepressive disorder
dc.subject.enanimal model
dc.subject.enlithium
dc.subject.engene expression
dc.subject.enAlox15
dc.titleDifferential expression profile between amygdala and blood during chronic lithium treatment in a rat model of depression – a pilot study
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue3
oaire.citation.volume82