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Serum Proteomic Analysis in Obese Postmenopausal Women Before and After Multi-Species Probiotic Supplementation

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cris.virtual.author-orcid0000-0002-4173-5965
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dc.abstract.enThe composition and functions of the gut microbiota can affect the development of obesity and metabolic disorders. Proteomic analysis has become an important tool for characterizing the proteome and identifying serum biomarkers associated with specific conditions in different groups of patients. The aim of this study was to gain insight into the pathophysiological aspects of obesity and the relationship between comorbidities and gut microbiota by characterizing the protein profile in obese postmenopausal women before and after multi-species probiotic supplementation. MALDI-TOF MS analyses were performed in serum samples from the placebo group (24 participants), low-dose probiotic group (24 participants), and high-dose probiotic group (23 participants). Paired comparison analysis of protein–peptide patterns derived from patients at two time points (i.e., before and after treatment) revealed several peaks that changed under treatment. Complement C3, fibrinogen alpha chain, coagulation factor XII, tubulin beta-3 chain, fibrinogen beta chain, inter-alpha-trypsin inhibitor heavy chain H4, and apolipoprotein A-I were identified. Slightly different alterations were observed depending on the administered dose. In summary, the obtained results do not allow us to draw a conclusion regarding the influence of probiotic supplementation on peptide profiles in obese postmenopausal women. However, some of the identified proteins may participate in the complex mechanism involving obesity, dysbiosis, and inflammation.
dc.affiliationWydział Nauk o Żywności i Żywieniu
dc.affiliation.instituteKatedra Żywienia Człowieka i Dietetyki
dc.contributor.authorKręgielska-Narożna, Matylda
dc.contributor.authorMiller-Kasprzak, Ewa
dc.contributor.authorMatuszewska, Eliza
dc.contributor.authorMatysiak, Jan
dc.contributor.authorSkrypnik, Katarzyna
dc.contributor.authorŁoniewski, Igor
dc.contributor.authorSzulińska, Monika
dc.contributor.authorBogdański, Paweł
dc.date.access2025-10-17
dc.date.accessioned2025-10-23T11:11:21Z
dc.date.available2025-10-23T11:11:21Z
dc.date.copyright2025-09-30
dc.date.issued2025
dc.description.abstract<jats:p>The composition and functions of the gut microbiota can affect the development of obesity and metabolic disorders. Proteomics analysis has become important tool for characterizing the proteome and identifying serum biomarkers associated with specific conditions in different groups of patients. The aim of this study was to gain insight into the pathophysiological aspects of obesity and the relation between comorbidities and the gut microbiota by characterizing the protein profile in obese post-menopausal women before and after multi-species probiotic supplementation. MALDI-TOF MS analyses were performed in serum samples from the placebo group 24, from the low-dose probiotic group 24, and 23 from the high-dose probiotic group. Paired comparison analysis of protein–peptide patterns derived from patients at two time points (i.e., before and after treatment) revealed several peaks changing under treatment. Complement C3, Fibrinogen alpha chain, Coagulation factor XII, Tubulin beta-3 chain, Fibrinogen beta chain, Inter-alpha-trypsin inhibitor heavy chain H4, and Apolipoprotein A-I were identified. Depending on the administered dose, slightly different alterations were observed. In summary, the obtained results do not allow us to draw a conclusion regarding the influence of probiotic supplementation on peptide profile in obese post-menopausal women. However some of identified proteins could take part in the complex mechanism involving obesity, dysbiosis, and inflammation. Registration number: NCT03100162</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if0,400
dc.description.number2
dc.description.points100
dc.description.versionfinal_published
dc.description.volume82
dc.identifier.doi10.32383/appdr/208794
dc.identifier.issn0001-6837
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/5418
dc.identifier.weblinkhttps://ptfarm.pl/wydawnictwa/czasopisma/acta-poloniae-pharmaceutica/110/-/30994
dc.languageen
dc.relation.ispartofActa Poloniae Pharmaceutica
dc.relation.pages263-273
dc.rightsCC-BY-NC
dc.sciencecloudnosend
dc.share.typeOTHER
dc.subject.enobesity
dc.subject.enserum proteomic profile
dc.subject.enMALDI-TOF MS
dc.subject.enprobiotics
dc.subject.ensupplementation
dc.titleSerum Proteomic Analysis in Obese Postmenopausal Women Before and After Multi-Species Probiotic Supplementation
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue2
oaire.citation.volume82