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Canine cystic endometrial hyperplasia and pyometra may downregulate neuropeptide phoenixin and GPR173 receptor expression

cris.virtual.author-orcid0000-0002-4563-3798
cris.virtual.author-orcid0000-0002-2198-6046
cris.virtual.author-orcid0000-0003-4059-1362
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cris.virtual.author-orcid0000-0001-5046-7416
cris.virtual.author-orcid0000-0002-1820-5202
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid0000-0003-2220-2730
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cris.virtualsource.author-orcid2887b0c9-4f57-4235-a68f-057f9a3211f9
cris.virtualsource.author-orcid2068f136-150d-448d-a014-c5b41eb5cb60
cris.virtualsource.author-orcid9118c61a-b066-4efc-a7af-486f9ebf9021
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid8cc943c4-fa09-421d-9e2b-84b33ec18db9
cris.virtualsource.author-orcid9fd4d04e-0912-425f-812f-aab2e3debe36
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cris.virtualsource.author-orcid71dccebf-e765-40b9-87bb-e98ab3b7299c
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.abstract.enThe most common uterine diseases affecting bitches are cystic endometrial hyperplasia (CEH) and pyometra. The neuropeptide phoenixin (PNX) and its receptor (GPR173) are potential key factors involved in the proliferative and inflammatory regulation of the reproductive system in females. This study aimed to evaluate the expression of PNX and GPR173 by qPCR, western blot and immunofluorescence assays in the endometrium of bitches suffering from CEH or pyometra compared to clinically healthy females. Additionally, PNX and progesterone (P4) plasma concentrations were analysed. The results showed a significantly lower expression levels of PNX and GPR173 (mRNA and protein production) in bitches with the CEH or pyometra groups compared to healthy animals. Immunofluorescence staining examination also confirmed a lower concentration of PNX and GPR173 signals in bitches with pathological uteri. Moreover, a lower concentration of PNX blood levels in bitches suffering from pyometra was observed. The PNX concentration was negatively correlated with P4 but only in healthy bitches. These results illustrate that the development of canine uterine disorders may cause a lower expression of PNX and its receptor GPR173.
dc.affiliationWydział Medycyny Weterynaryjnej i Nauk o Zwierzętach
dc.affiliation.instituteKatedra Nauk Przedklinicznych i Chorób Zakaźnych
dc.affiliation.instituteKatedra Fizjologii, Biochemii i Biostruktury Zwierząt
dc.affiliation.instituteKatedra Chorób Wewnętrznych i Diagnostyki
dc.affiliation.instituteKatedra Genetyki i Podstaw Hodowli Zwierząt​​
dc.contributor.authorRybska, Marta
dc.contributor.authorBillert, Maria
dc.contributor.authorSkrzypski, Marek
dc.contributor.authorKubiak, Magdalena
dc.contributor.authorWoźna-Wysocka, Magdalena
dc.contributor.authorŁukomska, Anna
dc.contributor.authorNowak, Tomasz
dc.contributor.authorBłaszczyk-Cichoszewska, Joanna
dc.contributor.authorPomorska-Mól, Małgorzata
dc.contributor.authorWąsowska, Barbara
dc.date.access2025-12-12
dc.date.accessioned2025-12-22T10:31:23Z
dc.date.available2025-12-22T10:31:23Z
dc.date.copyright2022-01-21
dc.date.issued2022
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if2,2
dc.description.numberMarch 2022
dc.description.points140
dc.description.versionfinal_published
dc.description.volume238
dc.identifier.doi10.1016/j.anireprosci.2022.106931
dc.identifier.eissn1873-2232
dc.identifier.issn0378-4320
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/6461
dc.identifier.weblinkhttps://www.sciencedirect.com/science/article/pii/S0378432022000100
dc.languageen
dc.relation.ispartofAnimal Reproduction Science
dc.relation.pagesart. 106931
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOTHER
dc.subject.encanine
dc.subject.enuterine diseases
dc.subject.enphoenixin
dc.subject.enGPR173 receptor
dc.titleCanine cystic endometrial hyperplasia and pyometra may downregulate neuropeptide phoenixin and GPR173 receptor expression
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.volume238