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The tissue distribution of nucleobindin-2/nesfatin-1 in the reproductive organs of bitches with regard to the animal’s age and body weight

cris.virtual.author-orcid0000-0002-4563-3798
cris.virtual.author-orcid0000-0003-4059-1362
cris.virtual.author-orcid0000-0002-3651-0972
cris.virtual.author-orcid0000-0001-9675-973X
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid2887b0c9-4f57-4235-a68f-057f9a3211f9
cris.virtualsource.author-orcid9118c61a-b066-4efc-a7af-486f9ebf9021
cris.virtualsource.author-orcid652e209d-434a-47b3-b5fb-64c0aac5a84c
cris.virtualsource.author-orcid1fe9b477-cfe7-4f84-8ce8-623637148ff1
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.abstract.enIntroduction: Neuropeptide nesfatin-1, a nucleobindin-2 derivative, plays a role in regulating food intake, energy metabolism and body weight. It also interacts with the hypothalamic-pituitary-gonadal axis and has functions in the reproductive system. However, its impact on the canine reproductive tract has not been well documented. This study aimed to investigate the potential role of nesfatin-1 in canine ovarian activity and uterine function. Material and Methods: Tissue and peripheral blood samples were collected from 60 bitches of various ages and body condition scores (BCS). Analyses included real-time PCR, immunofluorescence examinations and ELISA tests. Results: Higher level of nucleobindin-2 mRNA were found in the ovarian tissue of both younger and elderly overweight dogs (BCS > 5/9). The elevated expression of nesfatin-1 was observed in the uterine tissues of overweight dogs (BCS > 5/9) compared to its expression in animals in optimal body condition (BCS = 4/9). This finding was consistent with higher nesfatin-1 levels in the peripheral blood of overweight dogs. Conclusion: The distribution and expression of nesfatin-1 in canine reproductive organs vary depending on the animal’s age and body weight. The role of nesfatin-1 in the reproductive system is influenced by the animal’s body condition and the extent of surplus adipose tissue, which may have significant implications for reproductive functions.
dc.affiliationWydział Medycyny Weterynaryjnej i Nauk o Zwierzętach
dc.affiliationWydział Rolnictwa, Ogrodnictwa i Biotechnologii
dc.affiliation.instituteKatedra Nauk Przedklinicznych i Chorób Zakaźnych
dc.affiliation.instituteKatedra Fizjologii, Biochemii i Biostruktury Zwierząt
dc.affiliation.instituteKatedra Metod Matematycznych i Statystycznych
dc.contributor.authorRybska, Marta
dc.contributor.authorSkrzypski, Marek
dc.contributor.authorPusiak, Karolina
dc.contributor.authorWojciechowicz, Tatiana
dc.contributor.authorMieldzioc, Adam
dc.date.access2025-06-10
dc.date.accessioned2025-06-10T10:01:02Z
dc.date.available2025-06-10T10:01:02Z
dc.date.copyright2025-03-25
dc.date.issued2025
dc.description.abstract<jats:title>Abstract</jats:title> <jats:sec> <jats:title>Introduction</jats:title> <jats:p>Neuropeptide nesfatin-1, a nucleobindin-2 derivative, plays a role in regulating food intake, energy metabolism and body weight. It also interacts with the hypothalamic-pituitary-gonadal axis and has functions in the reproductive system. However, its impact on the canine reproductive tract has not been well documented. This study aimed to investigate the potential role of nesfatin-1 in canine ovarian activity and uterine function.</jats:p> </jats:sec> <jats:sec> <jats:title>Material and Methods</jats:title> <jats:p>Tissue and peripheral blood samples were collected from 60 bitches of various ages and body condition scores (BCS). Analyses included real-time PCR, immunofluorescence examinations and ELISA tests.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>Higher level of nucleobindin-2 mRNA were found in the ovarian tissue of both younger and elderly overweight dogs (BCS &gt; 5/9). The elevated expression of nesfatin-1 was observed in the uterine tissues of overweight dogs (BCS &gt; 5/9) compared to its expression in animals in optimal body condition (BCS = 4/9). This finding was consistent with higher nesfatin-1 levels in the peripheral blood of overweight dogs.</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusion</jats:title> <jats:p>The distribution and expression of nesfatin-1 in canine reproductive organs vary depending on the animal’s age and body weight. The role of nesfatin-1 in the reproductive system is influenced by the animal’s body condition and the extent of surplus adipose tissue, which may have significant implications for reproductive functions.</jats:p> </jats:sec>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_research
dc.description.financecost3000,00
dc.description.if1,3
dc.description.number1
dc.description.points140
dc.description.versionfinal_published
dc.description.volume69
dc.identifier.doi10.2478/jvetres-2025-0015
dc.identifier.eissn2450-8608
dc.identifier.issn2450-7393
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/2821
dc.identifier.weblinkhttps://sciendo.com/pl/article/10.2478/jvetres-2025-0015
dc.languageen
dc.relation.ispartofJournal of Veterinary Research (Poland)
dc.relation.pages141-150
dc.relation.projectPREIDUB
dc.rightsCC-BY-NC-ND
dc.sciencecloudsend
dc.share.typeOPEN_JOURNAL
dc.subject.enbody condition score
dc.subject.encanine
dc.subject.ennesfatin-1
dc.subject.enovary
dc.subject.enuterus
dc.titleThe tissue distribution of nucleobindin-2/nesfatin-1 in the reproductive organs of bitches with regard to the animal’s age and body weight
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue1
oaire.citation.volume69
project.funder.namesubwencja