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From Stress to Recovery: Divergent Chilling Responses in Contrasting Miscanthus sinensis Genotypes

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dc.abstract.enChilling temperatures are a major constraint on the early-season performance of C4 bioenergy crops in temperate regions. To dissect the temporal architecture of chilling resilience, we conducted an integrative, time-resolved analysis of two Miscanthus sinensis genotypes contrasting in chilling tolerance, Ms12 (LCT) and Ms16 (HCT). Through stepwise chilling and recovery treatments, we profiled genotype-specific changes in shoot physiology, hormone accumulation, gene expression, and importantly cell wall composition, a key yet understudied determinant of chilling resilience in perennial grasses. The high chilling-tolerant genotype (HCT) maintained its shoot growth, photosynthetic performance, and membrane stability by activating a delayed but sustained program involving secondary wall reinforcement, ABA–JA hormonal crosstalk, and raffinose family oligosaccharide (RFO) accumulation in response to the extreme conditions. While, low chilling-tolerant genotype (LCT) initiated a rapid transcriptional and hormonal response, which lacked persistence and failed to support structural recovery or metabolic buffering. In-depth transcriptomic profiling revealed divergent dynamics between studied genotypes. The LCT genotype mounted an early transcriptional burst, while the HCT genotype showed prolonged induction of the cell wall biosynthesis, energy metabolism, and stress-response genes. FTIR (Fourier-transform infrared spectroscopy) and sugar quantification confirmed genotype-specific remodeling of cell wall polymers. Moreover, hormone profiling showed that only the HCT genotype sustained ABA and JA signaling through the recovery process. RFOs accumulation, tightly linked to transcriptional activation of GolS (galactinol synthase) and RS (raffinose synthase) genes, was also more pronounced in the HCT genotype. Our findings demonstrate that chilling resilience in M. sinensis depends not on early response magnitude, but on the integration and temporal coordination of stress mitigation and recovery pathways. This work establishes a multiscale framework for identifying traits and regulatory modules underpinning chilling tolerance in perennial grasses, with direct relevance to climate-resilient biomass plant breeding
dc.affiliationWydział Leśny i Technologii Drewna
dc.affiliationWydział Nauk o Żywności i Żywieniu
dc.affiliation.instituteKatedra Chemicznej Technologii Drewna
dc.affiliation.instituteKatedra Biotechnologii i Mikrobiologii Żywności
dc.contributor.authorSobańska, Karolina
dc.contributor.authorGłowacka, Katarzyna
dc.contributor.authorKrajewski, Paweł
dc.contributor.authorWojtkowiak, Estera
dc.contributor.authorNuc, Maria
dc.contributor.authorBasińska‐Barczak, Aneta
dc.contributor.authorCzyż, Katarzyna B.
dc.contributor.authorWaligórski, Piotr
dc.contributor.authorKruszka, Dariusz
dc.contributor.authorGabała, Elżbieta
dc.contributor.authorGrzywaczyk, Adam
dc.contributor.authorZborowska, Magdalena
dc.contributor.authorDrożdżyńska, Agnieszka
dc.contributor.authorMokrzycka, Monika
dc.contributor.authorKoczyk, Grzegorz
dc.contributor.authorCerazy‐Waliszewska, Joanna
dc.contributor.authorMilewska‐Hendel, Anna
dc.contributor.authorBetekhtin, Alexander
dc.contributor.authorPniewski, Tomasz
dc.date.access2025-11-12
dc.date.accessioned2025-11-12T13:37:55Z
dc.date.available2025-11-12T13:37:55Z
dc.date.copyright2025-11-12
dc.date.issued2025
dc.description.abstract<jats:title>ABSTRACT</jats:title> <jats:p> Chilling temperatures are a major constraint on the early‐season performance of C <jats:sub>4</jats:sub> bioenergy crops in temperate regions. To dissect the temporal architecture of chilling resilience, we conducted an integrative, time‐resolved analysis of two <jats:styled-content style="fixed-case"> <jats:italic>Miscanthus sinensis</jats:italic> </jats:styled-content> genotypes contrasting in chilling tolerance, Ms12 (LCT) and Ms16 (HCT). Through stepwise chilling and recovery treatments, we profiled genotype‐specific changes in shoot physiology, hormone accumulation, gene expression, and importantly cell wall composition, a key yet understudied determinant of chilling resilience in perennial grasses. The high chilling‐tolerant genotype (HCT) maintained its shoot growth, photosynthetic performance, and membrane stability by activating a delayed but sustained program involving secondary wall reinforcement, ABA–JA hormonal crosstalk, and raffinose family oligosaccharide (RFO) accumulation in response to the extreme conditions. While, low chilling‐tolerant genotype (LCT) initiated a rapid transcriptional and hormonal response, which lacked persistence and failed to support structural recovery or metabolic buffering. In‐depth transcriptomic profiling revealed divergent dynamics between studied genotypes. The LCT genotype mounted an early transcriptional burst, while the HCT genotype showed prolonged induction of the cell wall biosynthesis, energy metabolism, and stress‐response genes. FTIR (Fourier‐transform infrared spectroscopy) and sugar quantification confirmed genotype‐specific remodeling of cell wall polymers. Moreover, hormone profiling showed that only the HCT genotype sustained ABA and JA signaling through the recovery process. RFOs accumulation, tightly linked to transcriptional activation of GolS (galactinol synthase) and RS (raffinose synthase) genes, was also more pronounced in the HCT genotype. Our findings demonstrate that chilling resilience in <jats:styled-content style="fixed-case"> <jats:italic>M. sinensis</jats:italic> </jats:styled-content> depends not on early response magnitude, but on the integration and temporal coordination of stress mitigation and recovery pathways. This work establishes a multiscale framework for identifying traits and regulatory modules underpinning chilling tolerance in perennial grasses, with direct relevance to climate‐resilient biomass plant breeding. </jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if4,1
dc.description.number12
dc.description.points140
dc.description.versionfinal_published
dc.description.volume17
dc.identifier.doi10.1111/gcbb.70087
dc.identifier.eissn1757-1707
dc.identifier.issn1757-1693
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/5852
dc.identifier.weblinkhttps://onlinelibrary.wiley.com/doi/10.1111/gcbb.70087
dc.languageen
dc.pbn.affiliationforestry
dc.relation.ispartofGCB Bioenergy
dc.relation.pagese70087
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOPEN_JOURNAL
dc.subject.enABA-JA crosstalk
dc.subject.encell wall remodeling
dc.subject.enchilling tolerance
dc.subject.enclimate resilience
dc.subject.enMiscanthus sinensis
dc.subject.enperennial C4 grass
dc.subject.enraffinose metabolism
dc.subject.entranscriptomic dynamics
dc.titleFrom Stress to Recovery: Divergent Chilling Responses in Contrasting Miscanthus sinensis Genotypes
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue12
oaire.citation.volume17
project.funder.nameSubwencja