Simple item page
 

Black Soldier Fly Meal as a Gastrointestinal Tract Microbiota Remodelling Factor: A New Natural and Sustainable Source of Prebiotic Substances for Fish?

cris.virtual.author-orcid0000-0001-8734-724X
cris.virtual.author-orcid0000-0002-3344-1094
cris.virtual.author-orcid0000-0001-6006-117X
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid0000-0002-6606-7975
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid1da983b0-605e-4368-b9d6-b560779a3cdc
cris.virtualsource.author-orcid8a0c5f30-f25c-446b-8092-83cb2446757a
cris.virtualsource.author-orcidaa495cd9-8e77-4c18-890a-9f4da0d598bc
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcidf5683666-5139-4173-800e-278b2903d57b
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.abstract.enThe microbiota of the gastrointestinal tract (GIT) plays a crucial role in the development, lifespan, physiology, barrier functions and immunity against pathogens in fish. One of the significant causes of fish mortality in production systems, which also generates huge financial losses, is pathogenic dieseases. Consequently, effectively managing disruptions in the microbiota could play a crucial role in mitigating economic losses across the sector. It was investigated the effect of an alternative raw material increasing inclusion—full-fat Hermetia illucens larvae meal on the composition of the gastrointestinal microbiome of Atlantic salmon. Thus, five experimental feeds were prepared: a control diet (CON) without the addition of black soldier fly full-fat meal (BSFM) and with 30% fish meal (FM); BSFM5, with 5% addition of full-fat BSFM and 27.1% FM; BSFM10, with 10% full-fat BSFM and 24.3% FM; BSFM15, with 15% addition of full-fat BSFM and 21.3% FM; and BSFM20, with 20% addition of full-fat BSFM and 18.6% FM. After 60 days of growth experiment on Atlantic salmon presmolts, the contents of two sections of the GIT, proximal and distal, were gently sampled and subjected to next-generation sequencing (NGS) to analyse the microbial populations. No significant differences were observed in the microbial compositions of the groups, irrespective of the intestinal section analysed. In the proximal and distal intestine, the CON group exhibited the highest number of distinct phyla. Increasing the inclusion of BSFM in the diet led to a reduction in the abundance of specific phyla. Similar results were noted at genus level. The BSFM5 and BSFM10 groups showed increase number in Enterococcus, while the Lactobacillus population were maintained at a high level. Due to abovementioned changes as well as the increased share of Bacilli populations, it may be concluded that up to 20% BSFM shares may have positive microbiota-modulating effects. Thus, BSFM may be considered not only as a nutrient source but a functional feed material in fish diets also, due to its prebiotic effects observed in the study.
dc.affiliationWydział Medycyny Weterynaryjnej i Nauk o Zwierzętach
dc.affiliation.instituteKatedra Zoologii
dc.affiliation.instituteKatedra Żywienia Zwierząt
dc.contributor.authorRawski, Mateusz
dc.contributor.authorMazurkiewicz, Jan
dc.contributor.authorMikołajczak, Zuzanna
dc.contributor.authorKierończyk, Bartosz
dc.contributor.authorSkrzypczak, Paula
dc.contributor.authorSzymkowiak, Piotr
dc.contributor.authorJózefiak, Damian
dc.date.access2025-08-19
dc.date.accessioned2025-08-20T07:08:05Z
dc.date.available2025-08-20T07:08:05Z
dc.date.copyright2025-02-01
dc.date.issued2025
dc.description.abstract<jats:p>The microbiota of the gastrointestinal tract (GIT) plays a crucial role in the development, lifespan, physiology, barrier functions and immunity against pathogens in fish. One of the significant causes of fish mortality in production systems, which also generates huge financial losses, is pathogenic dieseases. Consequently, effectively managing disruptions in the microbiota could play a crucial role in mitigating economic losses across the sector. It was investigated the effect of an alternative raw material increasing inclusion—full‐fat <jats:italic>Hermetia illucens</jats:italic> larvae meal on the composition of the gastrointestinal microbiome of Atlantic salmon. Thus, five experimental feeds were prepared: a control diet (CON) without the addition of black soldier fly full‐fat meal (BSFM) and with 30% fish meal (FM); BSFM5, with 5% addition of full‐fat BSFM and 27.1% FM; BSFM10, with 10% full‐fat BSFM and 24.3% FM; BSFM15, with 15% addition of full‐fat BSFM and 21.3% FM; and BSFM20, with 20% addition of full‐fat BSFM and 18.6% FM. After 60 days of growth experiment on Atlantic salmon presmolts, the contents of two sections of the GIT, proximal and distal, were gently sampled and subjected to next‐generation sequencing (NGS) to analyse the microbial populations. No significant differences were observed in the microbial compositions of the groups, irrespective of the intestinal section analysed. In the proximal and distal intestine, the CON group exhibited the highest number of distinct phyla. Increasing the inclusion of BSFM in the diet led to a reduction in the abundance of specific phyla. Similar results were noted at genus level. The BSFM5 and BSFM10 groups showed increase number in <jats:italic>Enterococcus</jats:italic>, while the <jats:italic>Lactobacillus</jats:italic> population were maintained at a high level. Due to abovementioned changes as well as the increased share of <jats:italic>Bacilli</jats:italic> populations, it may be concluded that up to 20% BSFM shares may have positive microbiota‐modulating effects. Thus, BSFM may be considered not only as a nutrient source but a functional feed material in fish diets also, due to its prebiotic effects observed in the study.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.versionfinal_published
dc.identifier.doi10.1155/are/8852384
dc.identifier.eissn1365-2109
dc.identifier.issn1355-557X
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/4302
dc.identifier.weblinkhttps://onlinelibrary.wiley.com/doi/full/10.1155/are/8852384?msockid=2f0cb6fbe02a6dd425c3a35fe10b6c38
dc.languageen
dc.pbn.affiliationanimal science and fisheries
dc.relation.ispartofAquaculture Research
dc.relation.pagesID 8852384
dc.relation.projectThis research was supported by the National Centre for Research and Development, no. POIR.01.01.01-00-0828/15, titled InnSecta: Innovative Technology of Feedstuffs Production Based on Insect Biomass, and the funds of Poznań University of Life Sciences
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOPEN_JOURNAL
dc.subject.enatlantic salmon nutrition
dc.subject.enblack soldier fly
dc.subject.eninnovative feed materials
dc.subject.eninsect meals
dc.subject.ensustainability
dc.subtypePublicationInPreprintService
dc.titleBlack Soldier Fly Meal as a Gastrointestinal Tract Microbiota Remodelling Factor: A New Natural and Sustainable Source of Prebiotic Substances for Fish?
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue1
oaire.citation.volume2025