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Field study on the utility of fluid obtained from testicles as a sample for detecting antibodies to selected swine pathogens

cris.virtual.author-orcid0000-0002-1610-0589
cris.virtual.author-orcid0000-0001-9874-6895
cris.virtual.author-orcid0000-0003-2220-2730
cris.virtualsource.author-orcidcfb82b96-7ef9-4b03-b4fb-1a3075fd805e
cris.virtualsource.author-orcid6fc589c0-a9dd-4835-bb57-7f31154633df
cris.virtualsource.author-orcid71dccebf-e765-40b9-87bb-e98ab3b7299c
dc.abstract.enProcessing fluid is a promising alternative to blood for monitoring porcine diseases, although certain aspects of its routine use remain unclear. This study evaluated serum from females and males, along with corresponding testicular only processing fluid, for antibodies against Actionbacillus pleuropneumonie, hepatitis E virus, porcine epidemic diarrhea virus, influenza A virus, Erysipetothrix rhusiopathie and Mycoplasma hyopneumoniae, using commercial ELISAs (ID Screen APP, Hepatitis E, PEDV, Influenza A from ID Vet, France; Civtest suis SE/MR from Hipra, Spain; and Mycoplasma hyopneumoniae from Idexx, USA). Differences in the proportion of positive results across sample types were analysed to assess the utility of testis-derived processing fluid for litter-level health monitoring. ROC analysis was used to establish optimal cut-offs for processing fluid, followed by evaluation of diagnostic performance using both manufacturer-recommended and ROC-derived thresholds. A pooling simulation was also performed. Results indicate that processing fluid collected exclusively from testes can detect antibodies against selected pathogens effectively. Some ELISA kits validated for serum may be applicable to processing fluid, provided that appropriate cut-off values are determined for this sample type. However, pooling processing fluid samples may reduce sensitivity and increase the risk of false-negative results. These findings highlight the potential of testis-derived processing fluid for large-scale serological surveillance while underscoring the need for test-specific validation.
dc.affiliationWydział Medycyny Weterynaryjnej i Nauk o Zwierzętach
dc.affiliation.instituteKatedra Nauk Przedklinicznych i Chorób Zakaźnych
dc.affiliation.instituteKatedra Chorób Wewnętrznych i Diagnostyki
dc.contributor.authorAugustyniak, Agata
dc.contributor.authorCzyżewska-Dors, Ewelina
dc.contributor.authorPomorska-Mól, Małgorzata
dc.date.access2025-09-23
dc.date.accessioned2025-09-30T12:28:29Z
dc.date.available2025-09-30T12:28:29Z
dc.date.copyright2025-07-01
dc.date.issued2025
dc.description.abstract<jats:title>Abstract</jats:title> <jats:p>Processing fluid is a promising alternative to blood for monitoring porcine diseases, although certain aspects of its routine use remain unclear. This study evaluated serum from females and males, along with corresponding testicular only processing fluid, for antibodies against <jats:italic>Actionbacillus pleuropneumonie</jats:italic>, hepatitis E virus, porcine epidemic diarrhea virus, influenza A virus, <jats:italic>Erysipetothrix rhusiopathie and Mycoplasma hyopneumoniae</jats:italic>, using commercial ELISAs (ID Screen APP, Hepatitis E, PEDV, Influenza A from ID Vet, France; Civtest suis SE/MR from Hipra, Spain; and Mycoplasma hyopneumoniae from Idexx, USA). Differences in the proportion of positive results across sample types were analysed to assess the utility of testis-derived processing fluid for litter-level health monitoring. ROC analysis was used to establish optimal cut-offs for processing fluid, followed by evaluation of diagnostic performance using both manufacturer-recommended and ROC-derived thresholds. A pooling simulation was also performed. Results indicate that processing fluid collected exclusively from testes can detect antibodies against selected pathogens effectively. Some ELISA kits validated for serum may be applicable to processing fluid, provided that appropriate cut-off values are determined for this sample type. However, pooling processing fluid samples may reduce sensitivity and increase the risk of false-negative results. These findings highlight the potential of testis-derived processing fluid for large-scale serological surveillance while underscoring the need for test-specific validation.</jats:p>
dc.description.accesstimeat_publication
dc.description.bibliographyil., bibliogr.
dc.description.financepublication_nocost
dc.description.financecost0,00
dc.description.if3,9
dc.description.points140
dc.description.versionfinal_published
dc.description.volume15
dc.identifier.doi10.1038/s41598-025-05380-8
dc.identifier.issn2045-2322
dc.identifier.urihttps://sciencerep.up.poznan.pl/handle/item/5087
dc.identifier.weblinkhttps://www.nature.com/articles/s41598-025-05380-8
dc.languageen
dc.pbn.affiliationveterinary science
dc.relation.ispartofScientific Reports
dc.relation.pagesart. 22402
dc.relation.projectNieinwazyjne strategie w monitorowaniu stanu zdrowia zwierząt. Badania nad przydatnością alternatywnych matryc w diagnostyce, epidemiologii i ocenie statusu immunologicznego na modelu świni domowej (Sus scrofa domestica)
dc.rightsCC-BY
dc.sciencecloudnosend
dc.share.typeOPEN_JOURNAL
dc.subject.enprocessing fluid
dc.subject.enantibodies
dc.subject.endetection
dc.subject.enELISA
dc.titleField study on the utility of fluid obtained from testicles as a sample for detecting antibodies to selected swine pathogens
dc.typeJournalArticle
dspace.entity.typePublication
oaire.citation.issue1
oaire.citation.volume15